LOCUS       sequence-342800-       10562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a human codon-optimized version of the 
            optogenetic GPCR parapinopsina (PP1) from zebrafish fused to 
            mCitrine. Also contains an N-terminal prolactin signal sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Bell GRR, Rincon E, Akdogan E, Collins SR
  TITLE     Optogenetic control of receptors reveals distinct roles for actin- 
            and Cdc42-dependent negative signals in chemotactic signal 
            processing.
  JOURNAL   Nat Commun. 2021 Nov 16;12(1):6148. doi: 10.1038/s41467-021-26371-z.
  PUBMED    34785668
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-11-16"; volume: "12(1):6148. doi"; pages: " 
            10.1038/s41467-021-26371-z"
COMMENT     Sequence Label: pLV_PP1-mCitrine
FEATURES             Location/Qualifiers
     source          1..10562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2220..2431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2432..3370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3327..3347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4532..4534,4535..4537,4538..4540,4541..5254)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(4580..4601)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4841..4860)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5188..5209
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5355..5904
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5499..5516)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5726..5745
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5907..6506
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPHTWCMTRKPGA"
     primer_bind     complement(5907..5926)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6403..6423
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6530..7118
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6583..6603)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7001..7012)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7325..7958
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8086..8102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8086..8102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8099..8121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8110..8126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8134..8140,8141..8158,8159..8164))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8179..8200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8317..8334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8488..9076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8568..8587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9247..10038,10039..10107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9870..9889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10108..10212)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10260..10394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10297..10316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10351..10370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10562,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccacgag aatggactct aagggcagca gccagaaggg
     3421 ctcccggctg ctgctcctcc tcgtggtgag caacctgctg ctctgccagg gggtggtgtc
     3481 tatgcacgag gagatggagt ccgagacctc tacagctgcc tctgggtcca ttgctgaggt
     3541 gatgccaaga atggggtaca caatcctcgc cgtgattatc ggggtgttct ccgtgtgcgg
     3601 agtgatcctc aacgtgacag tgatcacagt gaccctcaag tacaagcagc tgaggcagcc
     3661 cctcaacttc gccctggtga acctcgctgt ggctgacctc ggctgcgctg tgttcggagg
     3721 cctgcccacc gtggtgacca acgctatggg ctacttcagc ctgggacggg tggggtgcgt
     3781 gctggagggc ttcgctgtgg ccttcttcgg cattgctgcc ctctgctctg tggccgtgat
     3841 tgccctcgag cgctgcatgg tggtgtgccg cccagtgggc tccatttctt tccagacaag
     3901 gcacgctgtg ttcggagtgg ccgtgtcctg gctctggtcc ttcatctgga acacccctcc
     3961 cctcttcgga tgggggaggt tcgagctcga gggagtgaga acatcttgcg ctcctgactg
     4021 gtactctagg gacctggcca acgtgtcttt catcgtgtgc tacttcctgc tgtgcttcgc
     4081 cctcccattc agcgtgattg tgtactctta caccagactc ctgtggacac tgcggcaggt
     4141 gtctagactg caggtgtgcg agggggggtc cgctgctagg gctgaggctc aggtgtcctg
     4201 catggtggtg gtgatgattc tggccttcct cctgacatgg ctgccatacg ctagcttcgc
     4261 tctctgcgtg atcctcattc ccgagctgta cattgaccct gtgattgcta ccgtgcccat
     4321 gtacctgacc aagtcttcca cagtgttcaa ccctatcatc tacatcttca tgaacaggca
     4381 gttcagagac agagctctcc ccttcctcct gtgcggacgc aacccctggg ccgctgaggc
     4441 cgaggaggag gaggaggaga caaccgtgtc tagcgtgagc cggtccacct ccgtgtctcc
     4501 tgctgagaca tcccaggtgg cccctgctga aatgcatgtg agcaagggcg aggagctgtt
     4561 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4621 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4681 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
     4741 gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4801 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4861 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4921 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4981 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     5041 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5101 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     5161 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5221 gatcactctc ggcatggacg agctgtacaa gtgagcggcc gctctagagt cgacgggccg
     5281 cggtaacaat tgttaactaa cttaagctag caacggtttc cctctagcgg gatcaattcc
     5341 gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     5401 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     5461 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     5521 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     5581 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     5641 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     5701 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     5761 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     5821 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5881 tttcctttga aaaacacgat aataccatga ccgagtacaa gcccacggtg cgcctcgcca
     5941 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6001 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6061 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6121 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6181 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6241 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6301 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6361 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6421 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cacacctggt
     6481 gcatgacccg caagcccggt gcctgacccg ggcggggcgc gtctggaaca atcaacctct
     6541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6901 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7081 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     7141 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     7201 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     7261 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     7321 ggactggaag ggctaattcg ctcccaacga agacaagata tccttgatct gtggatctac
     7381 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     7441 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     7501 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     7561 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     7621 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     7681 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     7741 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     7801 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7861 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7921 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7981 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     8041 ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8101 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8161 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8221 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8281 gagaggcggt ttgtgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8341 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8401 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8461 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8521 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8581 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8641 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8701 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8761 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8821 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8881 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8941 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9001 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9061 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9121 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9181 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9241 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9301 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9361 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9421 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9481 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9541 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9601 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9661 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9721 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9781 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9841 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9901 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9961 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10021 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10081 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10141 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10201 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
    10261 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10321 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10381 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10441 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10501 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10561 tt
//
