LOCUS       sequence-342801-        3648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35G1_STOP
FEATURES             Location/Qualifiers
     source          1..3648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1205..1304)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1321..1340)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1322..1340)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1345..1361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1345..1361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1463..1482)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1474..2283
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1541..1560)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2430..3018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2919..2938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3172..3189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3348..3375)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3467..3553)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3602..3624
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3616..3633
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3617..3633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcg gccccaggac
      121 tccaccggag tggcagagct gcaggagcca ggactgcctc tgaccgacga tgccccccct
      181 ggcgccacag aggagcctgc agcagcagag gcagcaggag caccagatcg gggcagatgc
      241 tggctgtgcc tgagctcccc atgctgttcc agaaccgagc ccgaggccaa gaagaaggcc
      301 ccttgcccag gcctgggcct gttttacaca ctgctgtctg ccttcctgtt ttctgtgggc
      361 agcctgttcg tgaagaaggt gcaggacgtg cacgccgtgg agatcagcgc ctttcggtgc
      421 gtgttccaga tgctggtggt catcccctgt ctgatctata gaaagaccgg ctttatcggc
      481 cctaagggcc agaggatctt cctgatcctg aggggcgtgc tgggatctac cgccatgatg
      541 ctgatctact atgcctacca gacaatgagc ctggccgatg ccaccgtgat cacattttct
      601 agcccagtgt ttaccagcat cttcgcctgg atctgtctga aggagaagta ttccccctgg
      661 gacgccctgt ttacagtgtt caccatcaca ggcgtgatcc tgatcgtgag gccacccttc
      721 ctgtttggct ccgatacatc tggcatggag gagagctact ccggccacct gaagggcacc
      781 tttgccgcaa tcggatctgc cgtgttcgca gcaagcaccc tggtcatcct gcgcaagatg
      841 ggcaagtccg tggactactt cctgtctatc tggtactatg tggtgctggg cctggtggag
      901 agcgtgatca tcctgtccgt gctgggagag tggtccctgc cttattgcgg cctggatagg
      961 ctgtttctga tcttcatcgg cctgtttggc ctgggcggcc agatcttcat caccaaggcc
     1021 ctgcagatcg agaaggccgg ccctgtggcc atcatgaaga caatggacgt ggtgttcgcc
     1081 tttatcttcc agatcatctt ctttaacaat gtgccaacct ggtggacagt gggaggcgcc
     1141 ctgtgcgtgg tggcaagcaa cgtgggcgcc gccatccgga agtggtatca gtcctctaag
     1201 taagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1261 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1321 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1381 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1441 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1501 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1561 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1621 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1681 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1741 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1801 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1861 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1921 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     1981 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2041 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2101 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2161 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2221 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2281 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2341 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2401 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3001 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3061 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3121 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3181 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3241 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3301 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3361 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3421 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3481 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3541 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3601 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
