LOCUS       sequence-34282-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Yang J, Zhang Z, Zhang XA, Luo Q
  TITLE     A ligation-independent cloning method using nicking DNA 
            endonuclease.
  JOURNAL   Biotechniques. 2010 Nov . 49(5):817-21.
  PUBMED    21091446
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2010 Nov . 49(5):817-21."
COMMENT     Sequence Label: pLIC
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..100
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(104..112,113..127)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(256..273)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(256..272)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(265..287)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(481..500)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(585..603)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      599..646
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(830..849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaatattttg tttactttag aaggagatat acatatgcgg ggttctcatc atcatcatca
       61 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      121 cgataaggat ctgcctcagc aagggctgag gatgaccatg attaccaatt cgagctcggt
      181 acccggggat cctccatcgt tttcgtgggg tccatcgaaa tcgtggtcgt cgacctgcag
      241 gcatgcaaac ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      301 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgacga
      361 ggcccgcacc gatcgccctc cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      421 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgtatatggt gcactctcag
      481 tacaatctgc tctgatgccg catagcctca gcttccgctg aggaattcga agcttgatcc
      541 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      601 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      661 tatatccgga tctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      721 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      781 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      841 ttcttccctc ctttctcgcc acgtttcgcc ggctttcccc g
//
