LOCUS       sequence-34283-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Hayer A, Stoeber M, Ritz D, Engel S, Meyer HH, Helenius A
  TITLE     Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in
            endolysosomes for degradation.
  JOURNAL   J Cell Biol. 2010 Nov 1. 191(3):615-29.
  PUBMED    21041450
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Nov 1. 191(3):615-29."
COMMENT     Sequence Label: CAV1-mEGFP/FRT/TO
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(711..735)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    745..763
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    766..784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tctcgccctt gctcaccatg gtggcgaccg gtggatcccg ggcccgcggt gtttctttct
       61 gcatgttgat gcggatattg ctgaatattt tgccaacagc ctcaaagaac gggtcacaga
      121 aggtgtggac gtagatggaa tagacacggc tgatgcactg aatctcaatc aggaaactct
      181 taatgcacgg cacaactgcc cagatgtgca ggaaagaaag aatggcaaag taaatgcccc
      241 atatgagtgc cattgggatg ccaaagaggg cagacagcaa gcggtaaaac cagtattttg
      301 tcacagtgaa ggtggtgaag ctggccttcc agatgccatc aaaactgtgt gttccttctg
      361 gttctgcaat cacatcttca aaatcaatct tgaccacgtc gtcgttgaga tgcttggggt
      421 cgcggttgac caggtcgatt tccttggtgt gcgcgtcgta cacctgcttc tcgctcatct
      481 cctccgccat ggccttgttg ttgggcttgt agatgttgcc ctgctcccgg atgggaacgg
      541 tgtagaggtg cccctcggag tctacgtatt tgcccccaga catgctggcc ggtggctggg
      601 tganactcgt gccgaattcg aagcttaagt ttaaacgcta gagtccggag gctggatcgg
      661 tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg
      721 ttcactaaac gagctcgtcg acgatctcta tcactgatag ggagatctct atcactgata
      781 gggagagctc tg
//
