LOCUS       sequence-34284-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Hayer A, Stoeber M, Ritz D, Engel S, Meyer HH, Helenius A
  TITLE     Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in
            endolysosomes for degradation.
  JOURNAL   J Cell Biol. 2010 Nov 1. 191(3):615-29.
  PUBMED    21041450
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Nov 1. 191(3):615-29."
COMMENT     Sequence Label: CAV1-mEGFP-mCherry
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(698..722)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    732..750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    753..771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(773..872)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(802..822)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctctcgccc ttgctcactg gatcccgggc ccgcggtgtt tctttctgca tgttgatgcg
       61 gatattgctg aatattttgc caacagcctc aaagaacggg tcacagaagg tgtggacgta
      121 gatggaatag acacggctga tgcactgaat ctcaatcagg aaactcttaa tgcacggcac
      181 aactgcccag atgtgcagga aagaaagaat ggcaaagtaa atgccccata tgagtgccat
      241 tgggatgcca aagagggcag acagcaagcg gtaaaaccag tattttgtca cagtgaaggt
      301 ggtgaagctg gccttccaga tgccatcaaa actgtgtgtt ccttctggtt ctgcaatcac
      361 atcttcaaaa tcaatcttga ccacgtcgtc gttgagatgc ttggggtcgc ggttgaccag
      421 gtcgatttcc ttggtgtgcg cgtcgtacac ctgcttctcg ctcatctcct ccgccatggc
      481 cttgttgttg ggcttgtaga tgttgccctg ctcccggatg ggaacggtgt agaggtgccc
      541 ctcggagtct acgtatttgc ccccagacat gctggccggt ggctgggtga agctcgtgcc
      601 gaattcgaag cttaagttta aacgctagag tccggaggct ggatcggtcc cggtgtcttc
      661 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
      721 ctcgtcgacg atctctatca ctgataggga gatctctatc actgataggg agagctctgc
      781 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
      841 tacgacattt tggaaagtcc cgttgatttt ggtgccaaac
//
