LOCUS       sequence-342847-        3986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible gene expression, pCDF origin, CymRAM repressor, PCymRC 
            promoter, mRFP, Carbenicillin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3986)
  TITLE     Reisch Lab Duet Plasmids
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDCyR4
FEATURES             Location/Qualifiers
     source          1..3986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(77..815)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1025..1041
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1844..1871
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1902..1929
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     misc_RNA        1933..1982
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             2027..2038
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2045..2722
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        2957..3028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3029..3097,3098..3889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3247..3266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgaccaaaat ccaggtggca cttttcgggg agctagctca ctcggtcgct acgctccggg
       61 cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa
      121 gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg
      181 ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc
      241 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
      301 accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
      361 atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact
      421 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
      481 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc
      541 acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
      601 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
      661 tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt
      721 aaccttcgat caaaccacct acccaggtgg ttttttcgtt tacagggcaa aagattacgc
      781 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt
      841 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
      901 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
      961 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     1021 gcgcgtaaaa cgacggccag tgatcaacgt ttgaattttg cataacgttc acgtgcaatt
     1081 tccagggtgc tattacgcac acgttcaaaa cgttctttat ctttctgcca caggctacga
     1141 actaccagac cacgaacgct gttaaaaatc agccacagaa tatcttcggc atcatcacgg
     1201 ctcagaccac ggctcaccag cacacccagc cacatatctt caacaacaaa acgattacgt
     1261 tcaacggtac gctgaatacc ttcacgcagt gccggatcac gatctgctgc aacaatcaga
     1321 tccaggccga tgctaaaatc atcatccaga aaaaattctg ctgcatcatc cagcatctgc
     1381 tgaataacat catcttccgg tttcagtttt gccagacgtg cacggctacg ttcggtaatc
     1441 tgctcataca gccattcaaa ggttgccagc agcagttcca gtttggtcgg aaaatgatgg
     1501 ctctgtgcac cacggctaac accggctgca cccggaacat ctgcaatacg aaaacctgca
     1561 taaccttttt cacgcagaac acccagtgct gctgcaatca gtttaccctg ggtttccatt
     1621 gcacgttctg cctgggtacg acgtttcggg ctcataagaa tctccgtttg gtatttacta
     1681 tgaatattcc attgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggc
     1741 gcgccgcccc aattattgaa ggcctcccta acggggggcc tttttttgtt tctggtctcc
     1801 cgcttaacga tcgttggctg ggatacatat gtcaatgtac cggaacaaac agacaatctg
     1861 gtctgtttgt attatggaaa atttttctgt ataatagatt caacaaacag acaatctggt
     1921 ctgtttgtaa agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac
     1981 agcctctaca aataattttg tttaagaact gggaagagcg cagattaaag aggagaaata
     2041 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
     2101 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
     2161 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
     2221 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
     2281 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
     2341 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
     2401 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
     2461 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     2521 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     2581 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     2641 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     2701 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc gctttccagt
     2761 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2821 tgcgtattgg gcgcatgcat aaaaactgtt gtaattcatt aagcattctg ccgacatgga
     2881 agccatcaca aactgcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     2941 gcgtataata tttgcccatt caaatatgta tccgctcatg agacaataac cctgataaat
     3001 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3061 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3901 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3961 ggtgaagatc ctttttgata atctca
//
