LOCUS       sequence-342868-        7827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sMHT-113 fused non-dimerizing GRX2-C37A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Fulk EM, Huh D, Atkinson JT, Lie M, Masiello CA, Silberg JJ
  TITLE     A Split Methyl Halide Transferase AND Gate That Reports by 
            Synthesizing an Indicator Gas.
  JOURNAL   ACS Synth Biol. 2020 Nov 20;9(11):3104-3113. doi: 
            10.1021/acssynbio.0c00355. Epub 2020 Oct 26.
  PUBMED    33104325
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00355"; journalName: "ACS Synth Biol"; date: 
            "2020-11-20- 20"; volume: "9"; issue: "11"; pages: "3104-3113"
COMMENT     Sequence Label: pDH010
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             148..180
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        950..983
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             1001..1813
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKT
                     AFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDAS
                     DFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIA
                     DRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1001..1813
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKT
                     AFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDAS
                     DFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIA
                     DRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        1821..1839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1840..1864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2692..2709
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2762..2780)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2776..2823
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2860..3315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2947..2966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3157..3178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3407..4222)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3407..4222)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4214..4233
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4344..4932
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4833..4852
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5086..5103
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5118..5257
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5183..5202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5343..5365
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5359..5550)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6359..7441)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7403..7422
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7442..7519)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7725..7744
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gctagcatga ctggtggaca gcaaatgggt
      181 gcgggatcca tgagcaccgt ggcgaacatt gcgccggtgt ttaccggcga ttgcaaaacc
      241 attccgaccc cggaagaatg cgcgaccttt ctgtataaag tggtgaacag cggcggctgg
      301 gaaaaatgct gggtggaaga agtgattccg tgggatctgg gcgtgccgac cccgctggtg
      361 ctgcatctgg tgaaaaacaa cgcgctgccg aacggcaaag gcctggtgcc gggctgcggc
      421 ggcggctatg atgtggtggc gatggcgaac ccggaacgct ttatggtggg cctggatatt
      481 agcgaaaacg cgctgaaaaa agcgcgcgaa acctttagca ccatgccggc ggccgcgggt
      541 gcttctggtg gtggttcttc tggtggtcac atggagagca atacatcatc atctttggag
      601 aatttagcga cggcgcctgt gaaccagatc caagaaacaa tttctgataa ttgtgtggtg
      661 attttctcaa aaacatccgc atcttactgt acaatggcaa aaaagctttt ccatgacatg
      721 aatgttaact ataaagtggt ggaactggac ctgcttgaat atggaaacca gttccaagat
      781 gctctttaca aaatgactgg tgaaagaact gttccaagaa tatttgtcaa tggtactttt
      841 attggaggtg caactgacac tcataggctt cacaaagaag gaaaattgct cccactagtt
      901 catcaatgtt atttgaaaaa atctaagagg aaagaatttc agtgacaagt tacggctagc
      961 tcagtcctag gtatagtgct agcaggaggt gtactagttg agccatattc aacgggaaac
     1021 gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1081 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
     1141 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1201 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     1261 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca
     1321 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     1381 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     1441 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     1501 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     1561 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     1621 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     1681 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     1741 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     1801 tgagtttttc taactgcagt taatacgact cactataggg gaattgtgag cggataacaa
     1861 ttcccctcaa gaaataattt ttttaacttt aagaaggaga tattatgatg gagagcaaca
     1921 ccagtagcag tttggagaat ctcgcgacag caccggtgaa ccagattcag gaaacgatta
     1981 gcgataactg cgtagtcatt ttttcaaaaa cgtcggcgag ttattgcacc atggcaaaga
     2041 aactcttcca cgatatgaac gtcaactaca aagtcgtcga actggatctg ctggagtacg
     2101 gtaatcagtt tcaggatgcg ctgtacaaaa tgacaggcga gcgcacggtc ccgcgcattt
     2161 ttgttaatgg caccttcatc ggcggggcta ccgacaccca tcgccttcat aaagagggaa
     2221 agctgctgcc gttagtgcat caatgttatt tgaaaaaatc taaacgcaag gagtttcagg
     2281 gtgcttctgg tggtggttct tctggtggtc acatggcggc cgcaaacagc agctgcttta
     2341 gctttgtgaa agaagatgtg tttacctggc gcccggaaca gccgtttgat tttatttttg
     2401 attatgtgtt tttttgcgcg attgatccga aaatgcgccc ggcgtggggc aaagcgatgt
     2461 atgaactgct gaaaccggat ggcgaactga ttaccctgat gtatccgatt accaaccatg
     2521 aaggcggccc gccgtttagc gtgagcgaaa gcgaatatga aaaagtgctg gtgccgctgg
     2581 gctttaaaca gctgagcctg gaagattata gcgatctggc ggtggaaccg cgcaaaggca
     2641 aagaaaaact ggcgcgctgg aaaaaaatga acaactaaaa gcttactcga gcaccaccac
     2701 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     2761 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     2821 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
     2881 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2941 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3001 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3061 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3121 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3181 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3241 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3301 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3361 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
     3421 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     3481 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     3541 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     3601 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     3661 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     3721 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     3781 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     3841 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     3901 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     3961 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     4021 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     4081 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     4141 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     4201 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     4261 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4321 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4381 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4441 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4501 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4561 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4621 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4681 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4741 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4801 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4861 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4921 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4981 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5041 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5101 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     5161 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     5221 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     5281 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5341 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     5401 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     5461 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     5521 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     5581 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     5641 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     5701 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     5761 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     5821 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     5881 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     5941 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     6001 gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     6061 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     6121 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     6181 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     6241 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     6301 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     6361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6421 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     6481 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     6541 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     6601 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     6661 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     6721 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     6781 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     6841 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     6901 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     6961 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     7021 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     7081 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     7141 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     7201 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     7261 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     7321 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     7381 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     7441 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     7501 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
     7561 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     7621 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     7681 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     7741 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     7801 aggatcgaga tctcgatccc gcgaaat
//
