LOCUS       sequence-342879-       13430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible plasmid containing N-terminal luciferase for 
            split-luciferase complementation assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Yu G, Xian L, Xue H, Yu W, Rufian JS, Sang Y, Morcillo RJL, Wang Y, 
            Macho AP
  TITLE     A bacterial effector protein prevents MAPK-mediated phosphorylation 
            of SGT1 to suppress plant immunity.
  JOURNAL   PLoS Pathog. 2020 Sep 25;16(9):e1008933. doi: 
            10.1371/journal.ppat.1008933. eCollection 2020 Sep.
  PUBMED    32976518
REFERENCE   2  (bases 1 to 13430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-25"; volume: "16(9):e1008933. doi"; pages: 
            " 10.1371/journal.ppat.1008933. eCollection 2020 Sep"
COMMENT     Sequence Label: pGWB-nLUC
FEATURES             Location/Qualifiers
     source          1..13430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    195..319
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(356..361,362..379,380..386)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             440..1099
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(506..525)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1441..1746
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1625..1644
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1787..1911)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2027..2045)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      3218..3859
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3867..3884)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3867..3883)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3876..3898)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4086..4110
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5411..6040
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6469..7542
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7608..7802
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8038..8060)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8146..8286
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8201..8220
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8301..8318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8472..9060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8552..8571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9147..9941)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10366..10390
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10468..10642
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10683..11708)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11775..12451)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    12642..12663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12678..12683,12684..12701,12702..12708)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12716..12732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12721..12743
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12740..12756
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12740..12756
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        join(13265..13430,1..180)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
       61 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
      121 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca
      181 cgggggacga gctcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
      241 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
      301 aacatatcca gtcactatgg cggcccctcg aggggccgca ttaggcaccc caggctttac
      361 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
      421 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      481 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      541 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      601 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      661 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      721 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      781 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      841 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
      901 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
      961 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1021 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     1081 gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc
     1141 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
     1201 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     1261 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     1321 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     1381 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
     1441 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     1501 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
     1561 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     1621 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     1681 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     1741 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
     1801 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     1861 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgtcgacca
     1921 gatctcgtac gcgtcccggg gcggtggctc atctggcgga ggtgaagacg ccaaaaacat
     1981 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
     2041 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
     2101 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
     2161 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
     2221 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
     2281 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
     2341 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
     2401 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
     2461 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
     2521 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
     2581 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
     2641 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
     2701 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
     2761 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
     2821 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
     2881 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
     2941 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
     3001 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
     3061 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
     3121 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
     3181 gaccaacgcc ttgattgaca aggatggatg actgcagagc tttcgttcgt atcatcggtt
     3241 tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact
     3301 gagtttgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
     3361 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
     3421 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
     3481 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
     3541 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt
     3601 gtagttgtac cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc
     3661 tgcaaatgtt actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct
     3721 ttatgtaatt ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata
     3781 ctcatggatt tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt
     3841 gattgacaac atgcatcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     3901 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3961 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4021 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
     4081 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     4141 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     4201 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     4261 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     4321 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     4381 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     4441 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     4501 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     4561 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     4621 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     4681 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     4741 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     4801 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     4861 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     4921 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     4981 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     5041 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     5101 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     5161 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     5221 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     5281 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     5341 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     5401 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     5461 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     5521 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     5581 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     5641 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     5701 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     5761 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     5821 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     5881 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     5941 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     6001 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     6061 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     6121 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     6181 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     6241 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     6301 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     6361 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
     6421 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     6481 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     6541 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     6601 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     6661 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     6721 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     6781 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     6841 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     6901 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     6961 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     7021 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     7081 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     7141 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     7201 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     7261 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     7321 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     7381 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     7441 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     7501 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     7561 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg
     7621 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     7681 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     7741 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     7801 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     7861 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     7921 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     7981 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     8041 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8101 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     8161 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     8221 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8281 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8341 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8401 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8881 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9061 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9121 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
     9181 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     9241 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     9301 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     9361 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     9421 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     9481 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     9541 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     9601 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     9661 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     9721 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     9781 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     9841 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     9901 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     9961 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
    10021 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
    10081 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
    10141 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
    10201 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
    10261 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
    10321 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
    10381 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
    10441 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag
    10501 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
    10561 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
    10621 ctcacacatt attatggaga aactcgagct tgtcgatcga cagatcccgg tcggcatcta
    10681 ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
    10741 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
    10801 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
    10861 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
    10921 cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
    10981 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
    11041 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
    11101 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
    11161 agcatcagat catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
    11221 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
    11281 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
    11341 gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc
    11401 aggtcttgca acgtgacacc ctgtgaacgg cgggagatgc aataggtcag gctctcgcta
    11461 aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
    11521 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
    11581 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
    11641 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
    11701 tttttcatat ctcattgccc cccggatctg cgaaagctcg agagagatag atttgtagag
    11761 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
    11821 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
    11881 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
    11941 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
    12001 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
    12061 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
    12121 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
    12181 gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    12241 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
    12301 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
    12361 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    12421 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
    12481 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
    12541 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    12601 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    12661 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    12721 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc ggtccccaga
    12781 ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag
    12841 caggtctcat caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca
    12901 agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag
    12961 atatatttct caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca
    13021 aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg
    13081 ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac
    13141 agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg
    13201 agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg
    13261 caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag
    13321 ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc
    13381 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
//
