LOCUS       sequence-34288-e         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW FLIPglu-30u-Delta-13V
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     183..202
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        183..201
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    264..288
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(345..366)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatttatata aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat
       61 ctaccaacga tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt agacaagccg
      121 acaaccttga ttggagactt gaccaaacct ctggcgaaga agtccaaagc tggatccgcg
      181 cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacctgc
      241 agcggccatc atgaattaac tacaccactt tgtacaagaa agctgggtct agatagcttt
      301 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      361 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag
      421 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      481 gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaa
//
