LOCUS       sequence-342881-        5319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible gene expression, pET origin, LuxR activator, PLuxB 
            promoter, mRFP, Carbenicillin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5319)
  TITLE     Reisch Lab Duet Plasmids
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pELxR4
FEATURES             Location/Qualifiers
     source          1..5319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..46)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      42..89
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      126..581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(213..232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     423..444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(696..1487,1488..1556))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRKQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1319..1338
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1557..1649)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1730..2318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2219..2238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2472..2489
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2504..2643
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2569..2588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2729..2751
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2745..2936)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3508..3524
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        4502..4551
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             4596..4607
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4614..5291
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 gctttccagt cgggaaacct gctgccaccg ctgagcaata actagcataa ccccttgggg
       61 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      121 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      541 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg
      601 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      661 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      721 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      781 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      841 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      901 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttt ccgggaagct
      961 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1021 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1081 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1141 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1201 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1261 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1321 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1381 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1441 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1501 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1561 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1621 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt
     1681 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1741 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1801 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1861 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1921 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1981 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2041 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2101 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2161 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2221 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2281 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2341 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2401 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2461 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     2521 tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc
     2581 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     2641 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2701 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2761 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
     2821 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
     2881 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
     2941 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     3001 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     3061 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     3121 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
     3181 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
     3241 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     3301 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     3361 agccgggtcc tcaacgacag gagcacgatc atgctagtca tgccccgcgc ccaccggaag
     3421 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
     3481 agctaactta cattaattgc gttgcgcgta aaacgacggc cagtgatcaa tttttaaagt
     3541 atgggcaatc aattgctcct gttaaaattg ctttagaaat actttggcag cggtttgttg
     3601 tattgagttt catttgcgca ttggttaaat ggaaagtgac agtacgctca ctgcaaccta
     3661 atatttttga aatatcccaa gagctttttc cttcgcatgc ccacgctaaa cattcttttt
     3721 ctcttttggt taaatcgttg tttgatttat tatttgctat atttattttt cgataattat
     3781 caactagaga aggaacaatt aatggtatgt tcatacacgc atgtaaaaat aaactatcta
     3841 tatagttgtc tttttctgaa tgtgcaaaac taagcattcc gaagccattg ttagccgtat
     3901 gaatagggaa actaaaccca gtgataagac ctgatgtttt cgcttcttta attacatttg
     3961 gagatttttt atttacagca ttgttttcaa atatattcca attaattggt gaatgattgg
     4021 agttagaata atctactata ggatcatatt ttatcaaatt agcgtcatca taatattgcc
     4081 tccatttttt agggtaatta tctaggattg aaatatcaga tttaaccata gaatgaggat
     4141 aaatgatcgc gagtaaataa tattcacaat gtaccatttt agtcatatca gataagcatt
     4201 gattaatatc attattgctt ctacaagctt taattttatt aattattctg tatgtgtcgt
     4261 cggcatttat gtttttcatt ctgcccctcc gaaaggctta agtcctcgcg tagtacctgg
     4321 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggcgcgcc gcccaattat
     4381 tgaaggcctc cctaacgggg ggcctttttt tgtttctggt ctcccgctta acgatcgttg
     4441 gctgacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttacag tcgaataaaa
     4501 gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa
     4561 ataattttgt ttaagaactg ggaagagcgc agattaaaga ggagaaatac tagatggctt
     4621 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
     4681 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
     4741 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
     4801 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
     4861 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
     4921 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
     4981 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
     5041 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa
     5101 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
     5161 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
     5221 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
     5281 ccggtgctta ataacgctga tagtgctagt gtagatcgc
//
