LOCUS       sequence-342882-        4023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This gene was cloned into the gateway middle entry vector. It can be
            used for generating antisense riboprobe or gene expression using a 
            gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Silic MR, Murata SH, Park SJ, Zhang G
  TITLE     Evolution of inwardly rectifying potassium channels and their gene 
            expression in zebrafish embryos.
  JOURNAL   Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425.
  PUBMED    34558132
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Sep 23. doi: 10.1002/dvdy.425."
COMMENT     Sequence Label: pDONR221-kcnj3a
FEATURES             Location/Qualifiers
     source          1..4023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2148..2247)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2264..2283)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2265..2283)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2288..2304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2288..2304)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2406..2425)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2417..3226
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2484..2503)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3373..3961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3862..3881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc accatgtctg cactccgtaa gaaatttgga aatgactatc aggtagtgac
      721 cactacctcc tccagccatc tgaaggacaa gaggaggaaa cgacaacgat ttgtggataa
      781 gaacggacga tgtaacgtcc aacacggaaa cttgggcgga gagaccagca gatatctctc
      841 cgacttgttc acaactttag tagaccttaa atggaggtgg aaccttttca tcttcatcct
      901 cacatacaca gtggcctggt tattcatggc gtttatgtgg tgggtcattg catatatcag
      961 aggcgacctg aaccgaaccc atgatgagaa gtacacgcca tgcgtggcca acgtgtataa
     1021 ctttccctct gcgttcctct tttttattga gacggaggcc actataggat atggccacag
     1081 atacatcact gataaatgtc cagaagggat cattctcttc ttatttcagt ccatcctagg
     1141 ctccatagtg gatgcctttc taataggctg catgttcata aagatgtctc aacccaagaa
     1201 gagggcggaa actctgatgt tcagtgaaaa tgcagccatt tccatgcgtg acggcaaact
     1261 cacgctgatg ttcagagtgg gaaacctgcg caacagccac atggtttcag cccaaatccg
     1321 gtgtaaatta ctcaagtccc gtcagacgcc tgagggcgag ttccttcctt tggatcagct
     1381 ggagctggat gttggcttca gcactggagc ggaccagctc tttcttgtgt cgccactcac
     1441 aatctgccat gtgattgaca ctaagagccc cttctatgaa ctttcacaac actccatgca
     1501 gacagaacag ttcgaaattg tcgtgatttt ggagggtatt gttgaaacaa ctggcatgac
     1561 ttgtcaagcg cggacatcat acacagagga cgaggtgctt tggggccaca gattcttccc
     1621 agtcatctct ctagaggagg gcttctttaa agtggattat tctcagttcc atgccacatt
     1681 cgaagttccc acacctccct acagtgcaaa ggaacaggac gaggcattac tcatgtcctc
     1741 accactcatg gccccatcgc tgtgcaacag cggagaaaag aacagctccc ttgactgcct
     1801 agaactactg gaagatccag agagcaccac caaattacca gccaaagttc agaagatgac
     1861 aggtagagat gggcttccca ggaagctcct gagaatgagc tccaccacat cagagatgac
     1921 atacagcctt ggcgaactgc ctataaagct ccagcgcatc agttcagtgc ctggggtctc
     1981 agaggaaatg caggttacca agacgactag gatttccagc gaggccatca gcaaatcagt
     2041 tgcggacttg ccaccgaaac tacagagact gtctggtgga ggaggccgca tggatggaca
     2101 cctgccgccc aaactccgaa agatgaactc tgatcgcttc acataagacc cagctttctt
     2161 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2221 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2281 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2341 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2401 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2461 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2521 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2581 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2641 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2701 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2761 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2821 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2881 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2941 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3001 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3061 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3121 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3181 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3241 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3301 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3361 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3421 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3481 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3541 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3601 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3661 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3721 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3781 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3841 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3901 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3961 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4021 gtt
//
