LOCUS       sequence-34292-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW FLIPglu-2µ-Delta-13V
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     188..207
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        188..206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    269..293
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(308..559)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(350..371)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     699..718
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acttatttat aataaaaatc ataaatcata agaaattcgc ttatttagaa gtgtcaacaa
       61 cgtatctacc aacgatttga cccttttcca tcttttcgta aatttctggc aaggtagaca
      121 agccgacaac cttgattgga gacttgacca aacctctggc gaagaagtcc aaagctggat
      181 ccgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
      241 cctgcagcgg ccatcatgaa ttaactacac cactttgtac aagaaagctg ggtctagata
      301 gcttttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      361 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tggtagctca
      421 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      481 ggtcggcgag ctgcacgccg ccgtcctcga tgttgtggcg gatcttgaag ttggccttga
      541 tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg gctgttgtag ttgtactcca
      601 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      661 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
      721 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
      781 gctgcttcat gtggtcgggg tagcgggcga agcactgcag gc
//
