LOCUS       sequence-342923-       12656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible plasmid containing C-terminal luciferase for 
            split-luciferase complementation assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12656)
  AUTHORS   Yu G, Xian L, Xue H, Yu W, Rufian JS, Sang Y, Morcillo RJL, Wang Y, 
            Macho AP
  TITLE     A bacterial effector protein prevents MAPK-mediated phosphorylation 
            of SGT1 to suppress plant immunity.
  JOURNAL   PLoS Pathog. 2020 Sep 25;16(9):e1008933. doi: 
            10.1371/journal.ppat.1008933. eCollection 2020 Sep.
  PUBMED    32976518
REFERENCE   2  (bases 1 to 12656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-25"; volume: "16(9):e1008933. doi"; pages: 
            " 10.1371/journal.ppat.1008933. eCollection 2020 Sep"
COMMENT     Sequence Label: pGWB-cLUC
FEATURES             Location/Qualifiers
     source          1..12656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    384..405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(420..425,426..443,444..450)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    458..474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     463..485
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     482..498
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     482..498
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1007..1352
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1880..2004
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2041..2046,2047..2064,2065..2071)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2125..2784
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2191..2210)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3126..3431
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3310..3329
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3472..3596)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3616..4257
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(4265..4282)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4265..4281)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4274..4296)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4484..4508
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5809..6438
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6867..7940
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8006..8200
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8436..8458)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8544..8684
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8599..8618
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8699..8716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8870..9458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8950..8969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9545..10339)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10764..10788
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10866..11040
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11081..12106)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(join(12173..12656,1..193))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
       61 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
      121 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
      181 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
      241 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct
      301 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      361 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      421 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      481 acaggaaaca gctatgacat gattacgaat tcggtcccca gattagcctt ttcaatttca
      541 gaaagaatgc taacccacag atggttagag aggcttacgc agcaggtctc atcaagacga
      601 tctacccgag caataatctc caggaaatca aataccttcc caagaaggtt aaagatgcag
      661 tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca
      721 gaagtactat tccagtatgg acgattcaag gcttgcttca caaaccaagg caagtaatag
      781 agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag tcaaagattc
      841 aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata cagagtctct
      901 tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac acacttgtct
      961 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
     1021 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
     1081 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1141 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1201 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     1261 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     1321 tataaggaag ttcatttcat ttggagagaa cacgggggac gagctcatgt ccggttatgt
     1381 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     1441 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     1501 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     1561 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     1621 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     1681 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     1741 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     1801 caagaagggc ggaaagatcg ccgtgggagg tggctcatct ggcggaggtc agatctcgta
     1861 cgcgtcccgg ggcggtacca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
     1921 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     1981 aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg caccccaggc
     2041 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     2101 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     2161 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     2221 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     2281 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     2341 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     2401 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     2461 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     2521 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     2581 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     2641 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     2701 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     2761 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
     2821 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     2881 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     2941 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     3001 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     3061 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     3121 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     3181 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     3241 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     3301 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     3361 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     3421 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     3481 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     3541 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgtga
     3601 tgagtcgacc tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca
     3661 atgcatcagt ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg
     3721 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
     3781 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
     3841 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
     3901 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
     3961 ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat
     4021 tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag
     4081 tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct
     4141 tgtcagattc taatcattgc tttataatta tagttatact catggatttg tagttgagta
     4201 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct
     4261 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4321 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4381 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
     4441 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
     4501 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     4561 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     4621 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     4681 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     4741 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     4801 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     4861 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     4921 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     4981 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     5041 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     5101 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     5161 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     5221 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     5281 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     5341 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     5401 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     5461 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     5521 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     5581 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     5641 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     5701 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     5761 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     5821 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     5881 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     5941 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     6001 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     6061 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     6121 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     6181 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     6241 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     6301 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     6361 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     6421 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     6481 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     6541 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     6601 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     6661 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     6721 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     6781 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     6841 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     6901 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     6961 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     7021 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     7081 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     7141 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     7201 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     7261 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     7321 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     7381 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     7441 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     7501 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     7561 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     7621 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     7681 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     7741 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     7801 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     7861 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     7921 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     7981 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     8041 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     8101 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     8161 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     8221 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     8281 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     8341 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     8401 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8461 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8521 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     8581 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     8641 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     8701 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8761 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8821 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8881 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8941 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9001 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9061 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9301 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     9541 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     9601 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     9661 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     9721 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     9781 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     9841 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     9901 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     9961 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    10021 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    10081 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    10141 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    10201 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    10261 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    10321 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    10381 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    10441 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    10501 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    10561 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    10621 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    10681 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    10741 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
    10801 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
    10861 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
    10921 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
    10981 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
    11041 ctcgagcttg tcgatcgaca gatcccggtc ggcatctact ctatttcttt gccctcggac
    11101 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
    11161 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
    11221 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
    11281 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
    11341 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
    11401 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
    11461 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
    11521 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagatca tcgagagcct
    11581 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
    11641 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
    11701 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg
    11761 cgaccggttg tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
    11821 gtgaacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt
    11881 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
    11941 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
    12001 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
    12061 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc
    12121 cggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg
    12181 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
    12241 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
    12301 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
    12361 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
    12421 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
    12481 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
    12541 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca
    12601 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgct
//
