LOCUS       sequence-342926-       12711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion
            (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Lumaquin D, Johns E, Montal E, Weiss JM, Ola D, Abuhashem A, White 
            RM
  TITLE     An in vivo reporter for tracking lipid droplet dynamics in 
            transparent zebrafish.
  JOURNAL   Elife. 2021 Jun 11;10. pii: 64744. doi: 10.7554/eLife.64744.
  PUBMED    34114952
REFERENCE   2  (bases 1 to 12711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-06-11"; volume: "10. pii: 64744. doi"; pages: " 
            10.7554/eLife.64744"
COMMENT     Sequence Label: pDestTol2CG2--3.5ubb:plin2-tdtomato
FEATURES             Location/Qualifiers
     source          1..12711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(618..629,630..792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4756..4780
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6133..7563
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(7580..7604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7606..7740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7803..7823)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7831..7847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7863..7997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(8299..9012,9013..9015,9016..9018))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8302..9012,9013..9015,9016..9018))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8344..8365)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8693..8712
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8952..8973
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(10241..10428,10429..10440)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10474..10490)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10474..10490)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10527..10547)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10527..10545)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10566..10582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10579..10601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10590..10606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10614..10620,10621..10638,10639..10644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10659..10680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10797..10814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10968..11556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11048..11067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11727..12518,12519..12587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12350..12369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12588..12692)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt ggctccgaat tcgcccttaa aactcgagac cagcaaagtt
     1261 ctagaatttg tcgaaacatt tatgttatat atttcctgaa aaaaattctg agtaagttct
     1321 taagtgttat tgccagcaac ataaacaaca gacggcaaaa tgaataaatg ataacaaagc
     1381 agtaggctta aataaaccta atttttatag gctgttctct acaaccctca aacagtgatt
     1441 agttttgtac ttataaactt gccctttcat tcatatttca agaaaattgg ttcagaagat
     1501 ctggatattc tagcagttgt tcaagctcat ggagggatca gtgacctgat tccacaatga
     1561 ctaggcctaa tccagaaatt agatgactgt caacataaaa aggcacagca ctcactagct
     1621 gccctatata ttttattata ttttacatat attattttat ttatttagct ctgagtgctg
     1681 tactttctgg ttaaagaaaa ctgcttacaa cagctaacct gtactacctc aggctcaggg
     1741 aatttggaac aggtttgtct ggtttgtttc tttaaccatg catgcttgtt ttcaactatg
     1801 gcaacacagt cacatgggac attacagaaa tgatttgtcg atgacatgcg acttttcttt
     1861 aataaagcgc aaagatccca aaaagcaaac ttttaacaaa aatcatataa ttatattttc
     1921 aatccagctt tgtagcaact ttgtgctgct gttcactcag caacagatag tcagtataag
     1981 gtcagtgtgt ctcaaagcag tgccatctgt ttcacacatt gcgttctata tataagtgtg
     2041 ctggttgaca cgacactgta taaggcctag gctaaaacac aaacaatgta gaatgacact
     2101 gtgttttttt tgtaaacaaa tgttgttttt ggttaaacat ctttgtgaaa acatcctcct
     2161 gtcatgtatt tgctatattc aaatgttaaa cccgtgcaga atagaacata tacaaaaaaa
     2221 aacaacacaa cacattttta aacattatta aatatcaagt attgctggca gttctgtttc
     2281 tgttttacag taccctttgc cacagttctc cgcttttcct ggtccagatt ccacaagtct
     2341 gattcaccaa tagcaaagcg aataaacaac caaagcagcc aatcactgct tgtagactgt
     2401 cctgcgagac cggcccattc cagcacattc tggaaacttc ctttatatga taattataaa
     2461 tacatttaaa ttattgatac aaaacatgta attcctagaa cataaccata gcaatcatta
     2521 gttttcaggg taattatgta tttttaggat ttgactgcgg aaagatctgg tcatgtgacg
     2581 tctcatgaac gtcacggccc tgggtttcta taaatacagt aggactctcg accatcggca
     2641 gatttttcga agaagaagat cagtttcagg agccgtactg ttccgtttca acgcaaatat
     2701 taacggtaag agcgaatttc ctagtttgtt ttcatgccat tctttaaaac catagcgtat
     2761 tactttaatt atagtaaact ttcgctttct ttattacaag agacgttttg tgttgattct
     2821 ccgcggacat tttcggtcag acaatcagaa aatgaccgcg gaggaccagt aacttgcatt
     2881 acacgtaagt taaatcttcg tgtattaaaa tggttaggtt gttaacgtca aataggttac
     2941 cgtgtttgcg tgtgatcagg ttggttttgt tagatttttg tcagtatttt taatttattt
     3001 gttttagttt atttattttt tttgctgaat catagtttgt gaacaaagaa cccggatgtt
     3061 acatacagta cagccgccat gttacagaga gttataactt aatcatttta aaaataattt
     3121 tgccttactt ttagtttgtc atgttgagaa atgaggaaat gttaaaatga ggaaatatcc
     3181 aattaattta atatatcaaa ataatccatg attacaatgc actgaactgg agaaaattaa
     3241 gatgttttct agtgtcatga aacaaatgta agagatgtac attgtagatg ttttatgtca
     3301 agaattggct agttgatgca gcatactggc gatactcagt tgtaataaca gtaacgttac
     3361 atgttaatag actactgagt atgctgttct gtctatgtat gctctgtaag ctacgagaag
     3421 gactttttta aacagtaaag ggtgcaatat ttttacaaat tgaattaaat aaaggctgtc
     3481 tattaagtaa tatgcttgat atttttctta cttgatcgga aataagaaaa aatataaacg
     3541 ttgttgctct aaaaatccta gttcagttta gccaaccaca aatacctttt tgttcctcca
     3601 acagtttttt tttcttctct ataatatttg gcagtctata gtactccaaa tgtttcccca
     3661 cagtctaact aattggtaca gccaaaatca tgacacttat tgcaataata attttggttc
     3721 attggcattg ttgatagcct gtgccactaa tatggtcgat tgatcatgct tcaggaagaa
     3781 aactatattg tttgatgtaa gattattaaa tcttcacctg cttccattac aaactattcc
     3841 catcttattg aattctggta tgtcttaaag gattagttca cttcccaaat caaaatttac
     3901 ttagcctttt ttcatccatg atcccttttt ttcatcatta atgaagaaat tgtttttgaa
     3961 aaagtttcaa gatttttttc tctatattgt ggagcttgtt agtttaaaat tccaaaatgc
     4021 aatatgtggc ttcaaatggt tctaaatgat cccagtcaag gaataacagt cttatctaat
     4081 gaaaccatta gacctttaaa aataaaaaaa ataaaagtat ttatttttaa atgactgagt
     4141 gattaagttg aatttcagcg tttccttact gtgtagaagt ccttccttac tggccccacc
     4201 ctttggttct ctgccaatct gctacctaat gtaatgttgt ggaacattat tattctttat
     4261 ttcttaattt tttatttttt attttaaaac aatgtaaact gcacagatgt gcagtttgtt
     4321 taaaatggcc aatgctttgg aaatgcatga cataattaga tttcatgatg cacaaagcca
     4381 aatctcagag cttgtgcaaa atgagctatc atttcactag gtaagaccct aaattttcat
     4441 ataggatcat ttggacaatt ttgctgcagg taaaatgcat tctatagtcc actgtcagcc
     4501 attgttttgg atagtattta tttttctcta caagtatagt caatagtttt ctattatttt
     4561 aaaggtttgt aacatttaag ggtgaccaaa tgcaaagtaa aatttcattt tcgggtgaac
     4621 tatctcgttt aacatgggag aagtgcaaaa catacattat tggctagaac attgtagtat
     4681 tttttaaatg gaaatgtgtg attgctaatc ttactttgaa tttgtttaca gggatccttt
     4741 aagggcgaat tcgacccaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
     4801 atgagctttc ttctgtactt gaaactgcac aacagatttt cggctaacat gggttctatg
     4861 gaggatgtaa ataaccagaa tgtggttgcc cgaattgttc accttcccct ggttgtttcc
     4921 acctatgaca tgatgtcgaa cgtgtacgtc aacaccaaga acaaccaccc atacctgaag
     4981 tctgtgtgcg aggttgctga gaatggtgtc aaatccataa cctcagctgc ctttcacagc
     5041 gctttaccaa tcattggcaa actggagcct cagatctcta tggcaaatga cctggcgtgc
     5101 aaaggacttg ataagataga aaagactctg ccgatcctac acaagccatc tgaacagatt
     5161 atggccaatg ctaaagatgc agtgacagga gccaaggagg ctgtcagcgg gactgtgagc
     5221 ggtgccaagg agtctgtctc catcacccta aatgatgtga tggaccgaac acgcggggca
     5281 gtgcagggca gcgtggagaa gaccaagact gtggtcagcg gtggagtcca gacagtgatg
     5341 gacagcagga tgctcaaact ggtgtccagc agagtggaca atgcactgag cacatctgag
     5401 acgctgctgg agcactactt gcctgaaaat gaggaggaaa aagaaggtga agagaggaat
     5461 actgaaggat tggaaaatga cagcgatttg gccaattatt atgtccgact gggctctctg
     5521 tccattaaag tcagagacag gacttatcag agagccgtgg agaaagttcg tcatgccgtg
     5581 cagcgcggac aggagtccat ttcacagctt aaccacacca tggatataat cgaatttacc
     5641 aggaagaata ttgatgggtc taaccagaag ctgcaggaga aactgggttc actaatgggc
     5701 tggaagagcg tttctcaaag ccaagaaaca gagagcgaaa cagacagtga agctgagcaa
     5761 attgagtcgc gcacactgac catcgcccac agcctcagcc agcagcttca gagcacatgt
     5821 gtggtgctgg tgtccagtct caggggtctc cctcagcacg tccagctcca ggtgggctct
     5881 gtcagccgct ccgcattgga gatctacagc agcttcagta aagcggccgt gctgggggat
     5941 ctgtccagca ccgtcctgtc ctccagccgc tcacagctca gcagaatcac agagtccatg
     6001 gacaacgtga tggactacct gctcaacaac actcctctca actggctggt cggaccgttt
     6061 tacccacgtg tggagcgcgc cgttgaaaac gaggccaaat gtagcaaaca gaggaccctt
     6121 caagtcactg aaatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     6181 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     6241 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     6301 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     6361 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     6421 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     6481 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     6541 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     6601 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     6661 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     6721 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     6781 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
     6841 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
     6901 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     6961 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     7021 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     7081 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     7141 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     7201 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     7261 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     7321 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccgctac
     7381 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     7441 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     7501 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     7561 taaaagggtg ggcgcgccga cccagctttc ttgtacaaag tgggggatcc agacatgata
     7621 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     7681 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     7741 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     7801 tccaacttta ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg
     7861 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     7921 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     7981 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     8041 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     8101 atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag
     8161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8221 actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga
     8281 ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     8341 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     8401 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     8461 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     8521 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     8581 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     8641 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     8701 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     8761 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     8821 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     8881 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     8941 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     9001 ctcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac
     9061 tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt
     9121 ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc
     9181 tgataactgc acaagtgctc agattccagc agggtggaaa atgagataaa gtgtgcagat
     9241 ggggaggggg acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa
     9301 atagcccctt tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta
     9361 cctgtaaatg atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac
     9421 aaattcttaa ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt
     9481 gaattttctg gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga
     9541 catggaatga agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa
     9601 aataagagtg aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct
     9661 tgtacaaaca gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt
     9721 gcttggatgt atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc
     9781 taaagttaca actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat
     9841 gttaagtctg ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca
     9901 caactgattt aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc
     9961 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc
    10021 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa
    10081 tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata
    10141 atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg
    10201 caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa
    10261 gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa
    10321 tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc
    10381 ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg
    10441 gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca
    10501 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    10561 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10621 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10681 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10741 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10801 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10861 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10921 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10981 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11041 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11101 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11161 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11221 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11281 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11341 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11401 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11461 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11521 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11581 gggtctgacg ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca
    11641 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11701 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11761 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11821 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11881 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11941 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12001 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12061 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12121 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12181 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12241 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12301 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12361 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12421 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12481 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12541 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12601 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12661 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt g
//
