LOCUS       sequence-342927-       11929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion
            (lipid droplet reporter) with em7:bsd for blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Lumaquin D, Johns E, Montal E, Weiss JM, Ola D, Abuhashem A, White 
            RM
  TITLE     An in vivo reporter for tracking lipid droplet dynamics in 
            transparent zebrafish.
  JOURNAL   Elife. 2021 Jun 11;10. pii: 64744. doi: 10.7554/eLife.64744.
  PUBMED    34114952
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-06-11"; volume: "10. pii: 64744. doi"; pages: " 
            10.7554/eLife.64744"
COMMENT     Sequence Label: pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato
FEATURES             Location/Qualifiers
     source          1..11929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(324..511,512..523)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(557..573)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(557..573)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(610..630)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(610..628)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(649..665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(662..684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    673..689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(697..703,704..721,722..727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    742..763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(880..897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1051..1639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1131..1150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1810..2601,2602..2670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2433..2452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2671..2775)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2938..2959)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(3308..3319,3320..3482)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     3855..3871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3891..3911
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7446..7470
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8823..10253
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(10270..10294)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10296..10430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10493..10513)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10521..10537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10553..10687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10809..11138
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10989..11124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11051..11070
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11186..11233
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11252..11650
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11808..11929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 agatctgcga agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct
       61 gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat
      121 aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt
      181 cacttccaaa ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc
      241 ataggcagaa taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg
      301 taatagcaag ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa
      361 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt
      421 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa
      481 gtacagtaat caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc
      541 ttcagcaagc ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag
      601 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
      661 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      721 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      781 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1681 gaacgaaaac tcacgttaag gcattttggt catgagatta tcaaaaagga tcttcaccta
     1741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     1981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     2821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3001 gtctatcagg gcgatggccc acgctggcta agaactcatc agcctccccg gtccatctac
     3061 ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc attccttcct
     3121 ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga gtacgcctac
     3181 ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga
     3241 atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag
     3301 ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt
     3361 agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa
     3421 gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag
     3481 aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca
     3541 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
     3601 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     3661 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     3721 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     3781 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     3841 gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat caactttgta
     3901 tagaaaagtt ggctccgaat tcgcccttaa aactcgagac cagcaaagtt ctagaatttg
     3961 tcgaaacatt tatgttatat atttcctgaa aaaaattctg agtaagttct taagtgttat
     4021 tgccagcaac ataaacaaca gacggcaaaa tgaataaatg ataacaaagc agtaggctta
     4081 aataaaccta atttttatag gctgttctct acaaccctca aacagtgatt agttttgtac
     4141 ttataaactt gccctttcat tcatatttca agaaaattgg ttcagaagat ctggatattc
     4201 tagcagttgt tcaagctcat ggagggatca gtgacctgat tccacaatga ctaggcctaa
     4261 tccagaaatt agatgactgt caacataaaa aggcacagca ctcactagct gccctatata
     4321 ttttattata ttttacatat attattttat ttatttagct ctgagtgctg tactttctgg
     4381 ttaaagaaaa ctgcttacaa cagctaacct gtactacctc aggctcaggg aatttggaac
     4441 aggtttgtct ggtttgtttc tttaaccatg catgcttgtt ttcaactatg gcaacacagt
     4501 cacatgggac attacagaaa tgatttgtcg atgacatgcg acttttcttt aataaagcgc
     4561 aaagatccca aaaagcaaac ttttaacaaa aatcatataa ttatattttc aatccagctt
     4621 tgtagcaact ttgtgctgct gttcactcag caacagatag tcagtataag gtcagtgtgt
     4681 ctcaaagcag tgccatctgt ttcacacatt gcgttctata tataagtgtg ctggttgaca
     4741 cgacactgta taaggcctag gctaaaacac aaacaatgta gaatgacact gtgttttttt
     4801 tgtaaacaaa tgttgttttt ggttaaacat ctttgtgaaa acatcctcct gtcatgtatt
     4861 tgctatattc aaatgttaaa cccgtgcaga atagaacata tacaaaaaaa aacaacacaa
     4921 cacattttta aacattatta aatatcaagt attgctggca gttctgtttc tgttttacag
     4981 taccctttgc cacagttctc cgcttttcct ggtccagatt ccacaagtct gattcaccaa
     5041 tagcaaagcg aataaacaac caaagcagcc aatcactgct tgtagactgt cctgcgagac
     5101 cggcccattc cagcacattc tggaaacttc ctttatatga taattataaa tacatttaaa
     5161 ttattgatac aaaacatgta attcctagaa cataaccata gcaatcatta gttttcaggg
     5221 taattatgta tttttaggat ttgactgcgg aaagatctgg tcatgtgacg tctcatgaac
     5281 gtcacggccc tgggtttcta taaatacagt aggactctcg accatcggca gatttttcga
     5341 agaagaagat cagtttcagg agccgtactg ttccgtttca acgcaaatat taacggtaag
     5401 agcgaatttc ctagtttgtt ttcatgccat tctttaaaac catagcgtat tactttaatt
     5461 atagtaaact ttcgctttct ttattacaag agacgttttg tgttgattct ccgcggacat
     5521 tttcggtcag acaatcagaa aatgaccgcg gaggaccagt aacttgcatt acacgtaagt
     5581 taaatcttcg tgtattaaaa tggttaggtt gttaacgtca aataggttac cgtgtttgcg
     5641 tgtgatcagg ttggttttgt tagatttttg tcagtatttt taatttattt gttttagttt
     5701 atttattttt tttgctgaat catagtttgt gaacaaagaa cccggatgtt acatacagta
     5761 cagccgccat gttacagaga gttataactt aatcatttta aaaataattt tgccttactt
     5821 ttagtttgtc atgttgagaa atgaggaaat gttaaaatga ggaaatatcc aattaattta
     5881 atatatcaaa ataatccatg attacaatgc actgaactgg agaaaattaa gatgttttct
     5941 agtgtcatga aacaaatgta agagatgtac attgtagatg ttttatgtca agaattggct
     6001 agttgatgca gcatactggc gatactcagt tgtaataaca gtaacgttac atgttaatag
     6061 actactgagt atgctgttct gtctatgtat gctctgtaag ctacgagaag gactttttta
     6121 aacagtaaag ggtgcaatat ttttacaaat tgaattaaat aaaggctgtc tattaagtaa
     6181 tatgcttgat atttttctta cttgatcgga aataagaaaa aatataaacg ttgttgctct
     6241 aaaaatccta gttcagttta gccaaccaca aatacctttt tgttcctcca acagtttttt
     6301 tttcttctct ataatatttg gcagtctata gtactccaaa tgtttcccca cagtctaact
     6361 aattggtaca gccaaaatca tgacacttat tgcaataata attttggttc attggcattg
     6421 ttgatagcct gtgccactaa tatggtcgat tgatcatgct tcaggaagaa aactatattg
     6481 tttgatgtaa gattattaaa tcttcacctg cttccattac aaactattcc catcttattg
     6541 aattctggta tgtcttaaag gattagttca cttcccaaat caaaatttac ttagcctttt
     6601 ttcatccatg atcccttttt ttcatcatta atgaagaaat tgtttttgaa aaagtttcaa
     6661 gatttttttc tctatattgt ggagcttgtt agtttaaaat tccaaaatgc aatatgtggc
     6721 ttcaaatggt tctaaatgat cccagtcaag gaataacagt cttatctaat gaaaccatta
     6781 gacctttaaa aataaaaaaa ataaaagtat ttatttttaa atgactgagt gattaagttg
     6841 aatttcagcg tttccttact gtgtagaagt ccttccttac tggccccacc ctttggttct
     6901 ctgccaatct gctacctaat gtaatgttgt ggaacattat tattctttat ttcttaattt
     6961 tttatttttt attttaaaac aatgtaaact gcacagatgt gcagtttgtt taaaatggcc
     7021 aatgctttgg aaatgcatga cataattaga tttcatgatg cacaaagcca aatctcagag
     7081 cttgtgcaaa atgagctatc atttcactag gtaagaccct aaattttcat ataggatcat
     7141 ttggacaatt ttgctgcagg taaaatgcat tctatagtcc actgtcagcc attgttttgg
     7201 atagtattta tttttctcta caagtatagt caatagtttt ctattatttt aaaggtttgt
     7261 aacatttaag ggtgaccaaa tgcaaagtaa aatttcattt tcgggtgaac tatctcgttt
     7321 aacatgggag aagtgcaaaa catacattat tggctagaac attgtagtat tttttaaatg
     7381 gaaatgtgtg attgctaatc ttactttgaa tttgtttaca gggatccttt aagggcgaat
     7441 tcgacccaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgagctttc
     7501 ttctgtactt gaaactgcac aacagatttt cggctaacat gggttctatg gaggatgtaa
     7561 ataaccagaa tgtggttgcc cgaattgttc accttcccct ggttgtttcc acctatgaca
     7621 tgatgtcgaa cgtgtacgtc aacaccaaga acaaccaccc atacctgaag tctgtgtgcg
     7681 aggttgctga gaatggtgtc aaatccataa cctcagctgc ctttcacagc gctttaccaa
     7741 tcattggcaa actggagcct cagatctcta tggcaaatga cctggcgtgc aaaggacttg
     7801 ataagataga aaagactctg ccgatcctac acaagccatc tgaacagatt atggccaatg
     7861 ctaaagatgc agtgacagga gccaaggagg ctgtcagcgg gactgtgagc ggtgccaagg
     7921 agtctgtctc catcacccta aatgatgtga tggaccgaac acgcggggca gtgcagggca
     7981 gcgtggagaa gaccaagact gtggtcagcg gtggagtcca gacagtgatg gacagcagga
     8041 tgctcaaact ggtgtccagc agagtggaca atgcactgag cacatctgag acgctgctgg
     8101 agcactactt gcctgaaaat gaggaggaaa aagaaggtga agagaggaat actgaaggat
     8161 tggaaaatga cagcgatttg gccaattatt atgtccgact gggctctctg tccattaaag
     8221 tcagagacag gacttatcag agagccgtgg agaaagttcg tcatgccgtg cagcgcggac
     8281 aggagtccat ttcacagctt aaccacacca tggatataat cgaatttacc aggaagaata
     8341 ttgatgggtc taaccagaag ctgcaggaga aactgggttc actaatgggc tggaagagcg
     8401 tttctcaaag ccaagaaaca gagagcgaaa cagacagtga agctgagcaa attgagtcgc
     8461 gcacactgac catcgcccac agcctcagcc agcagcttca gagcacatgt gtggtgctgg
     8521 tgtccagtct caggggtctc cctcagcacg tccagctcca ggtgggctct gtcagccgct
     8581 ccgcattgga gatctacagc agcttcagta aagcggccgt gctgggggat ctgtccagca
     8641 ccgtcctgtc ctccagccgc tcacagctca gcagaatcac agagtccatg gacaacgtga
     8701 tggactacct gctcaacaac actcctctca actggctggt cggaccgttt tacccacgtg
     8761 tggagcgcgc cgttgaaaac gaggccaaat gtagcaaaca gaggaccctt caagtcactg
     8821 aaatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     8881 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     8941 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     9001 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     9061 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     9121 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     9181 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     9241 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     9301 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     9361 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     9421 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     9481 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     9541 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     9601 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     9661 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     9721 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     9781 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     9841 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     9901 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     9961 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
    10021 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccgctac ctggtggagt
    10081 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
    10141 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
    10201 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taaaagggtg
    10261 ggcgcgccga cccagctttc ttgtacaaag tgggggatcc agacatgata agatacattg
    10321 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
    10381 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
    10441 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tccaacttta
    10501 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga
    10561 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
    10621 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
    10681 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
    10741 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
    10801 tcagatctgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    10861 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    10921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
    10981 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    11041 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
    11101 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
    11161 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
    11221 agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc
    11281 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
    11341 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
    11401 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
    11461 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
    11521 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
    11581 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
    11641 ggagggctaa gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
    11701 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    11761 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    11821 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    11881 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatctta
//
