LOCUS       sequence-342929-       13089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A modified pYEDP60 plasmid containing a yeast codon-optimized 
            version of human ATP13A2 variant 2, followed by a thrombin cleavage 
            site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   van Veen S, Martin S, Van den Haute C, Benoy V, Lyons J, Vanhoutte 
            R, Kahler JP, Decuypere JP, Gelders G, Lambie E, Zielich J, Swinnen 
            JV, Annaert W, Agostinis P, Ghesquiere B, Verhelst S, Baekelandt V, 
            Eggermont J, Vangheluwe P
  TITLE     ATP13A2 deficiency disrupts lysosomal polyamine export.
  JOURNAL   Nature. 2020 Feb;578(7795):419-424. doi: 10.1038/s41586-020-1968-7. 
            Epub 2020 Jan 29.
  PUBMED    31996848
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-1968-7"; journalName: "Nature"; date: "2020-02";
            volume: "578"; issue: "7795"; pages: "419-424"
COMMENT     Sequence Label: pYeDP60-hATP13A2-WT
FEATURES             Location/Qualifiers
     source          1..13089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3826..3843
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      4245..4515
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(4539..4555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4539..4555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4552..4574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4563..4579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4587..4593,4594..4611,4612..4617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4632..4653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(6403..6778)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(6971..6988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7142..7730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7222..7241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7901..8692,8693..8761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8524..8543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8762..8866)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8934..8952
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8990..9012)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9112..9131
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9325..9347
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9339..9356
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9340..9356
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9921..11263
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10884..10931)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        11644..11859
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11860..12663
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     protein_bind    12920..13037
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 aaggtttttt taggctaatc gacctcgagc agatccgcca ggcgtgtata tagcgtggat
       61 ggccaggcaa ctttagtgct gacacataca ggcatatata tatgtgtgcg acgacacatg
      121 atcatatggc atgcatgtgc tctgtatgta tataaaactc ttgttttctt cttttctcta
      181 aatattcttt ccttatacat taggtccttt gtagcataaa ttactatact tctatagaca
      241 cgcaaacaca aatacacaca ctaaattacc gaattcaaaa aaatgtctgc agactctagc
      301 cctttagtcg gatctacacc aacgggctac ggcacattga caattggtac gtctattgat
      361 cctctatctt ccagtgtgtc tagtgttagg ctgagtgggt attgtggttc cccctggagg
      421 gtaatcggtt atcatgtggt tgtctggatg atggcgggta tacctctgct gttattccgt
      481 tggaaaccat tgtggggtgt gagacttaga ttgagacctt gtaacttggc ccacgctgaa
      541 actttggtaa tcgaaattag agacaaagaa gattccagtt ggcagctatt tactgttcaa
      601 gttcaaactg aagctattgg tgagggctct ttagaaccaa gtccccaatc tcaagccgaa
      661 gacggtagat ctcaagctgc tgttggtgct gtgccggaag gtgcttggaa ggatacagcg
      721 cagttacata aatcagaaga ggcaaagaga gttctaagat attacttatt ccaaggtcaa
      781 agatatattt ggattgagac tcaacaggcc ttttatcaag ttagcttgtt ggatcatgga
      841 agatcttgtg atgacgtaca tagatccaga catggtctat ctttacagga ccagatggtt
      901 cgtaaagcaa tctatggtcc caacgttata tctataccag taaagagtta tcctcaatta
      961 ctggtcgatg aggccttaaa tccctattat ggatttcagg cgttttctat agcgctttgg
     1021 ttagccgacc attattactg gtacgctcta tgcatctttt taatatcctc tatctctatt
     1081 tgtttatcat tatacaaaac tcgtaaacag tcacaaaccc taagagatat ggtcaaactt
     1141 tctatgaggg tatgcgtctg tagaccggga ggtgaagaag aatgggtaga ttcctctgaa
     1201 cttgtgccag gcgactgttt ggtcttgcct caagagggtg gattaatgcc atgcgatgca
     1261 gcgcttgtcg ccggcgaatg catggtgaat gaaagctccc tgacaggtga atccatacca
     1321 gttctgaaga ctgcattgcc tgaagggtta ggtccatact gcgccgagac ccataggaga
     1381 catacattat tctgtggaac ccttattttg caggctagag cgtatgttgg tccacatgtt
     1441 ctagctgttg ttactagaac aggattttgc acggcaaaag gtggccttgt gtctagtatt
     1501 ttgcatccac gtccaattaa tttcaaattc tacaagcact ctatgaagtt cgttgccgct
     1561 ctttcagtgt tggcattgct gggtacaata tactctatat tcattctgta cagaaacaga
     1621 gtacccttaa atgaaattgt tataagggca ttggaccttg tcactgtggt cgtacctcca
     1681 gctttgccag ccgccatgac agtttgtacg ttatatgccc aatccagact aaggagacaa
     1741 ggcatctttt gtattcaccc attaaggata aatcttgggg gtaaactaca gctagtatgc
     1801 tttgacaaaa caggtacttt aactgaagac ggcttggacg taatgggtgt cgttccgtta
     1861 aagggtcaag cgttccttcc tttggtccca gaaccaagga gacttcctgt tggtccactt
     1921 ctgagggcat tggctacttg ccacgctcta agtagattgc aagatactcc ggtgggtgat
     1981 ccaatggact tgaaaatggt cgagagtacg ggctgggtat tagaggaaga acccgctgct
     2041 gattccgcct tcggtactca agtattagca gttatgagac cgccattgtg ggaaccccaa
     2101 ctgcaagcta tggaagaacc acctgttccg gtaagcgtgt tgcataggtt tcctttctca
     2161 tccgctttgc agagaatgag tgtcgttgtc gcatggccgg gcgcaaccca accagaagca
     2221 tacgtaaagg ggtctcctga gttggtagcc ggtctttgta accccgaaac ggttccaacg
     2281 gactttgcac agatgttgca aagctacaca gcggcaggtt atcgtgttgt cgcattggca
     2341 tctaaaccat tgcctacagt accctctctg gaagcggccc aacaattaac aagagatact
     2401 gtggaaggtg atttgtcctt gttgggttta ctagtcatgc gtaatttgtt gaaacctcaa
     2461 accaccccgg tcattcaggc tctaagaagg accagaatta gggcagtgat ggtcactggc
     2521 gataatctac aaacggctgt gacagttgca cgtggttgtg gtatggtcgc accacaagaa
     2581 cacttaatca ttgtgcatgc gactcatcct gaacgtggtc agcccgcatc cttggagttc
     2641 ctacctatgg aatctccaac tgccgtaaat ggtgttaaag atccagatca agcggcaagc
     2701 tacactgttg agcccgatcc tcgttctaga catttggcct tgtctggccc aaccttcggt
     2761 attattgtga aacattttcc aaaattgctg ccaaaggttt tggtccaggg gaccgttttt
     2821 gctcgtatgg cgcctgagca gaaaactgaa cttgtctgcg agttgcaaaa attgcaatat
     2881 tgcgtcggta tgtgtgggga tggggctaat gactgtggag cgctgaaagc tgcagatgta
     2941 ggtatctcct tatcacaagc tgaagctagt gtagttagtc catttactag ttcaatggca
     3001 agtatagaat gtgtgccaat ggtcataaga gaaggtaggt gtagtctaga tacttctttt
     3061 tctgttttca agtatatggc tttgtacagc ttgactcaat ttattagtgt cttaattctg
     3121 tatactatta atactaactt gggcgatttg caatttctag ctatagactt agtgattacc
     3181 actacagttg ccgttttaat gtctagaacc ggccctgctt tagttctagg aagggtgaga
     3241 cccccaggtg cattactgtc agtcccagtt ttgagctcac tattgctaca gatggtcctt
     3301 gtcacaggtg tgcaactagg cggatatttt ctgacgttag ctcaaccctg gtttgtccca
     3361 ttgaacagaa ctgttgctgc cccagacaat ctgcctaact atgaaaatac ggtagttttc
     3421 agcttatcta gctttcagta tttaatccta gcagccgctg tgtccaaagg agcacctttc
     3481 agaaggccct tatacaccaa tgttcccttt ttagtggctc tagcgttgtt gtccagcgta
     3541 ctggtaggtc tagttttagt acccggctta ttacagggcc ccttggcttt aaggaatatt
     3601 actgacactg gttttaagtt gttacttttg ggattagtaa cattaaactt tgttggtgca
     3661 ttcatgttgg aatctgttct tgatcaatgc ctgccagctt gcttgcgtag acttagacca
     3721 aagagagcga gtaaaaagag attcaagcaa ttagaaagag aattagcaga acaaccttgg
     3781 ccccctctac cagctggtcc attgagagcg ggtggtggtg gtagtctagt tccacgtggt
     3841 agtacggcgg ctgctcccgg gcctgctcct gctcctgctc ctgcctctgc acccgccgct
     3901 gccgccccgg ccggtgccgg caccccggtg accgccccgc tggcgggcac tatctggaag
     3961 gtgctggcca gcgaaggcca gacggtggcc gcaggcgagg tgctgctgat tctggaagcc
     4021 atgaagatgg aaaccgaaat ccgcgccgcg caggccggga ccgtgcgcgg tatcgcggtg
     4081 aaagccggcg acgcggtggc ggtcggcgac accctgatga cctaagagct cggtacccgg
     4141 atcagcgggg gatctcccat gtctctactg gtggtggtgc ttctttggaa ttattggaag
     4201 gtaaggaatt gccaggtgtt gctttcttat ccgaaaagaa ataaattgaa ttgaattgaa
     4261 atcgatagat caattttttt cttttctctt tccccatcct ttacgctaaa ataatagttt
     4321 attttatttt ttgaatattt tttatttata tacgtatata tagactatta tttatctttt
     4381 aatgattatt aagattttta ttaaaaaaaa attcgctcct cttttaatgc ctttatgcag
     4441 tttttttttc ccattcgata tttctatgtt cgggttcagc gtattttaag tttaataact
     4501 cgaaaattct gcgttcgtta aagcttggcg taatcatggt catagctgtt tcctgtgtga
     4561 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     4621 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     4681 cagtcgggaa acctgtcgtg ccagagcttc gtaaccgaca gtttctaact tttgtgcttt
     4741 gacaagaact tcttcttctt gctttaataa aaactgttcc attttcgttg tataacttga
     4801 atcataagcg ccaagcagtc tgacagccaa cagcgcagcg ttcgtactat tattaatagc
     4861 gacggtagct actggaacac ctctaggcat ttgcacaatt gaatgtaaag aatctactcc
     4921 atctagacaa gaacctttta cgggcacacc gatgacagga agtggtgtca ttgcagccac
     4981 catacctggc aagtgagcag ccccaccagc tccagcgata attgttttaa ttccacgctt
     5041 gcttgcggaa atagcatatg ctgacatcct atgtggagtt ctatgagcag agactattgt
     5101 cacttcaaat ggaacgccaa aatcttttaa aaccgcacat gcggcagaca ttaccggcaa
     5161 gtcagagtct gatcccatga tgattccaac caatggtttg accattgctt ccaagtccaa
     5221 cttttgagcg acagagattt tgattggaat atcagttcta cctgtaatgt agttcagcct
     5281 ttgttcacat tccgccatac tggaggcaat aatatttatg tgacctactt ttctgttagg
     5341 tctagactct tttccatata agtacactga ggaacctgga gtcgccaatg ctctttcgca
     5401 agtttctagc tctttatctt ttgtatgttt gtctccaaga acatttagca taatggcgtt
     5461 cgttgtaatg gtggagaaag atgtgaaatt ctttggcatt ggcaaatcca atattgatct
     5521 caaatgagct tcaaattgag aagtgacgca agcatcaatg gtataatgtc cagagttgtg
     5581 aggccttggg gcaatttcgt taataagcaa ttcccctgtt tctaaataga acatttccac
     5641 accaaatata ccacaaccgg gaaaagattt gattgcattt tctgccaaca acttcgcctt
     5701 aagttgaacg gagtccggaa ctctagcagg cgcataacat aagtcacaaa tattgtcctt
     5761 gtggatagtc tctacaattg ggtaagaaaa cactaaaccg ttaacagatc tcacaatcat
     5821 gactgctaat tctttagtaa atggtgccca tttttcggcg tacaaaggac gatccttcag
     5881 tacttccaaa gcttccggaa tcatttcctt attctttaca acgaagttac ctcttccatc
     5941 gtatgccaaa gtcctcgact tcaagacgaa tggaaaaccc aaatctcttc caacattcaa
     6001 tagggacgtc tcactggctt gttccacagg aacactttgg gtaactgcta taccattttt
     6061 gattaaatgc tctttttgaa tatatttgtc ttgtatcaat ctgattgttt ctggagaagg
     6121 gtaaattttt aatttgggat gttttacttg aagattcttt agtgtaggaa catcaacatg
     6181 ctcaatctca atcgttagca catcacattt ttcagctagt ttttcgatat caagaggatt
     6241 ggaaaaggag ccattaacgt ggtcattgga gttgcttatt tgtttggcag gagaattttc
     6301 agcatctagt attaccgtct taatgttgag cctgtttgct gcctcaacaa tcatacgtcc
     6361 caattgtccc cctcctaata taccaactgt tctagaatcc atacttgatt gttttgtccg
     6421 attttcttgt ttttcttgat tgttatagta ggatgtactt agaagagaga tccaacgatt
     6481 ttacgcacca atttatacat gaaatgctcc ataatattgt ccatttagtt cttaataaaa
     6541 ggtcagcaag agtcaatcac ttagtattac ccggttcgta gccatgcaac aagagtcatt
     6601 tgtcagcata gctgtaataa tcaatcatga cgtaagaaat gtatcataat taaaagttgt
     6661 taaagatgtc agtgttatgt tggtgttaca aaattctcgg cttctcacta atatttaata
     6721 tctcttaaat tttatctgtc tttgattctt ttaagaaaag ttatgtatta ttcaagaaaa
     6781 agtcaattcc gcatcaaaag gtaaaattta tataaactgc tttaaaattt catgaaacta
     6841 ggcaactttt cgaaatgatc tttttcgagc atgaagtttc ttttataata acctggtcaa
     6901 aagctctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8881 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8941 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     9001 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     9061 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     9121 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     9181 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     9241 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     9301 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     9361 taattccacg gactatagac tatactagta tactccgtct actgtacgat acacttccgc
     9421 tcaggtcctt gtcctttaac gaggccttac cactcttttg ttactctatt gatccagctc
     9481 agcaaaggca gtgtgatcta agattctatc ttcgcgatgt agtaaaacta gctagaccga
     9541 gaaagagact agaaatgcaa aaggcacttc tacaatggct gccatcatta ttatccgatg
     9601 tgacgctgca gcttctcaat gatattcgaa tacgctttga ggagatacag cctaatatcc
     9661 gacaaactgt tttacagatt tacgatcgta cttgttaccc atcattgaat tttgaacatc
     9721 cgaacctggg agttttccct gaaacagata gtatatttga acctgtataa taatatatag
     9781 tctagcgctt tacggaagac aatgtatgta tttcggttcc tggagaaact attgcatcta
     9841 ttgcataggt aatcttgcac gtcgcatccc cggttcattt tctgcgtttc catcttgcac
     9901 ttcaatagca tatctttgtt aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
     9961 acgcgagaac gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
    10021 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
    10081 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
    10141 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
    10201 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
    10261 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
    10321 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
    10381 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
    10441 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
    10501 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
    10561 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
    10621 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
    10681 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
    10741 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
    10801 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg
    10861 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
    10921 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
    10981 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
    11041 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
    11101 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
    11161 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
    11221 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatatgca tagtaccgag
    11281 aaactagtgc gaagtagtga tcaggtattg ctgttatctg atgagtatac gttgtcctgg
    11341 ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac tccgttaggc
    11401 ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt gggccatttt
    11461 ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc tttattgtac gatctgacta
    11521 agttatcttt taataattgg tattcctgtt tattgcttga agaattgccg gtcctattta
    11581 ctcgttttag gactggttca gaattaattc tcatgtttga cagcttatca tcgcgataag
    11641 cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc ttgaaatttt
    11701 tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg
    11761 gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa
    11821 ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag
    11881 gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa
    11941 aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta
    12001 gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat
    12061 ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta
    12121 ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg
    12181 ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca
    12241 ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt
    12301 ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt
    12361 actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac
    12421 atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat
    12481 gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga
    12541 tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag
    12601 ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac
    12661 taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt
    12721 taattatatc agttattacc cgatcaaaaa tcatcgcttc gctgattaat taccccagaa
    12781 ataaggctaa aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt
    12841 gaaggtttgt ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta
    12901 ttgtagaatc tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg
    12961 cgaccggtga agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct
    13021 cggcggcttc taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt
    13081 tccaaagag
//
