LOCUS       sequence-342930-       13089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A modified pYEDP60 plasmid containing a yeast codon-optimized 
            version of human ATP13A2 variant 2 E343A mutant, followed by a 
            thrombin cleavage site and a C-term BAD tag. DOI: 
            10.21769/BioProtoc.3888.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   van Veen S, Martin S, Van den Haute C, Benoy V, Lyons J, Vanhoutte 
            R, Kahler JP, Decuypere JP, Gelders G, Lambie E, Zielich J, Swinnen 
            JV, Annaert W, Agostinis P, Ghesquiere B, Verhelst S, Baekelandt V, 
            Eggermont J, Vangheluwe P
  TITLE     ATP13A2 deficiency disrupts lysosomal polyamine export.
  JOURNAL   Nature. 2020 Feb;578(7795):419-424. doi: 10.1038/s41586-020-1968-7. 
            Epub 2020 Jan 29.
  PUBMED    31996848
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-1968-7"; journalName: "Nature"; date: "2020-02";
            volume: "578"; issue: "7795"; pages: "419-424"
COMMENT     Sequence Label: pYeDP60-hATP13A2-E343A
FEATURES             Location/Qualifiers
     source          1..13089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3964..3981
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      4383..4653
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(4677..4693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4677..4693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4690..4712)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4701..4717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4725..4731,4732..4749,4750..4755))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4770..4791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(6541..6916)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(7109..7126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7280..7868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7360..7379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8039..8830,8831..8899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8662..8681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8900..9004)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9072..9090
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9128..9150)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9250..9269
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9463..9485
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9477..9494
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9478..9494
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10059..11401
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11022..11069)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        11782..11997
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11998..12801
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     protein_bind    join(13058..13089,1..86)
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 tcaggaacgc gaccggtgaa gacgaggacg cacggaggag agtcttcctt cggagggctg
       61 tcacccgctc ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt
      121 actgaaagtt ccaaagagaa ggttttttta ggctaatcga cctcgagcag atccgccagg
      181 cgtgtatata gcgtggatgg ccaggcaact ttagtgctga cacatacagg catatatata
      241 tgtgtgcgac gacacatgat catatggcat gcatgtgctc tgtatgtata taaaactctt
      301 gttttcttct tttctctaaa tattctttcc ttatacatta ggtcctttgt agcataaatt
      361 actatacttc tatagacacg caaacacaaa tacacacact aaattaccga attcaaaaaa
      421 atgtctgcag actctagccc tttagtcgga tctacaccaa cgggctacgg cacattgaca
      481 attggtacgt ctattgatcc tctatcttcc agtgtgtcta gtgttaggct gagtgggtat
      541 tgtggttccc cctggagggt aatcggttat catgtggttg tctggatgat ggcgggtata
      601 cctctgctgt tattccgttg gaaaccattg tggggtgtga gacttagatt gagaccttgt
      661 aacttggccc acgctgaaac tttggtaatc gaaattagag acaaagaaga ttccagttgg
      721 cagctattta ctgttcaagt tcaaactgaa gctattggtg agggctcttt agaaccaagt
      781 ccccaatctc aagccgaaga cggtagatct caagctgctg ttggtgctgt gccggaaggt
      841 gcttggaagg atacagcgca gttacataaa tcagaagagg caaagagagt tctaagatat
      901 tacttattcc aaggtcaaag atatatttgg attgagactc aacaggcctt ttatcaagtt
      961 agcttgttgg atcatggaag atcttgtgat gacgtacata gatccagaca tggtctatct
     1021 ttacaggacc agatggttcg taaagcaatc tatggtccca acgttatatc tataccagta
     1081 aagagttatc ctcaattact ggtcgatgag gccttaaatc cctattatgg atttcaggcg
     1141 ttttctatag cgctttggtt agccgaccat tattactggt acgctctatg catcttttta
     1201 atatcctcta tctctatttg tttatcatta tacaaaactc gtaaacagtc acaaacccta
     1261 agagatatgg tcaaactttc tatgagggta tgcgtctgta gaccgggagg tgaagaagaa
     1321 tgggtagatt cctctgaact tgtgccaggc gactgtttgg tcttgcctca agagggtgga
     1381 ttaatgccat gcgatgcagc gcttgtcgcc ggcgaatgca tggtgaatga aagctccctg
     1441 acaggtgctt ccataccagt tctgaagact gcattgcctg aagggttagg tccatactgc
     1501 gccgagaccc ataggagaca tacattattc tgtggaaccc ttattttgca ggctagagcg
     1561 tatgttggtc cacatgttct agctgttgtt actagaacag gattttgcac ggcaaaaggt
     1621 ggccttgtgt ctagtatttt gcatccacgt ccaattaatt tcaaattcta caagcactct
     1681 atgaagttcg ttgccgctct ttcagtgttg gcattgctgg gtacaatata ctctatattc
     1741 attctgtaca gaaacagagt acccttaaat gaaattgtta taagggcatt ggaccttgtc
     1801 actgtggtcg tacctccagc tttgccagcc gccatgacag tttgtacgtt atatgcccaa
     1861 tccagactaa ggagacaagg catcttttgt attcacccat taaggataaa tcttgggggt
     1921 aaactacagc tagtatgctt tgacaaaaca ggtactttaa ctgaagacgg cttggacgta
     1981 atgggtgtcg ttccgttaaa gggtcaagcg ttccttcctt tggtcccaga accaaggaga
     2041 cttcctgttg gtccacttct gagggcattg gctacttgcc acgctctaag tagattgcaa
     2101 gatactccgg tgggtgatcc aatggacttg aaaatggtcg agagtacggg ctgggtatta
     2161 gaggaagaac ccgctgctga ttccgccttc ggtactcaag tattagcagt tatgagaccg
     2221 ccattgtggg aaccccaact gcaagctatg gaagaaccac ctgttccggt aagcgtgttg
     2281 cataggtttc ctttctcatc cgctttgcag agaatgagtg tcgttgtcgc atggccgggc
     2341 gcaacccaac cagaagcata cgtaaagggg tctcctgagt tggtagccgg tctttgtaac
     2401 cccgaaacgg ttccaacgga ctttgcacag atgttgcaaa gctacacagc ggcaggttat
     2461 cgtgttgtcg cattggcatc taaaccattg cctacagtac cctctctgga agcggcccaa
     2521 caattaacaa gagatactgt ggaaggtgat ttgtccttgt tgggtttact agtcatgcgt
     2581 aatttgttga aacctcaaac caccccggtc attcaggctc taagaaggac cagaattagg
     2641 gcagtgatgg tcactggcga taatctacaa acggctgtga cagttgcacg tggttgtggt
     2701 atggtcgcac cacaagaaca cttaatcatt gtgcatgcga ctcatcctga acgtggtcag
     2761 cccgcatcct tggagttcct acctatggaa tctccaactg ccgtaaatgg tgttaaagat
     2821 ccagatcaag cggcaagcta cactgttgag cccgatcctc gttctagaca tttggccttg
     2881 tctggcccaa ccttcggtat tattgtgaaa cattttccaa aattgctgcc aaaggttttg
     2941 gtccagggga ccgtttttgc tcgtatggcg cctgagcaga aaactgaact tgtctgcgag
     3001 ttgcaaaaat tgcaatattg cgtcggtatg tgtggggatg gggctaatga ctgtggagcg
     3061 ctgaaagctg cagatgtagg tatctcctta tcacaagctg aagctagtgt agttagtcca
     3121 tttactagtt caatggcaag tatagaatgt gtgccaatgg tcataagaga aggtaggtgt
     3181 agtctagata cttctttttc tgttttcaag tatatggctt tgtacagctt gactcaattt
     3241 attagtgtct taattctgta tactattaat actaacttgg gcgatttgca atttctagct
     3301 atagacttag tgattaccac tacagttgcc gttttaatgt ctagaaccgg ccctgcttta
     3361 gttctaggaa gggtgagacc cccaggtgca ttactgtcag tcccagtttt gagctcacta
     3421 ttgctacaga tggtccttgt cacaggtgtg caactaggcg gatattttct gacgttagct
     3481 caaccctggt ttgtcccatt gaacagaact gttgctgccc cagacaatct gcctaactat
     3541 gaaaatacgg tagttttcag cttatctagc tttcagtatt taatcctagc agccgctgtg
     3601 tccaaaggag cacctttcag aaggccctta tacaccaatg ttcccttttt agtggctcta
     3661 gcgttgttgt ccagcgtact ggtaggtcta gttttagtac ccggcttatt acagggcccc
     3721 ttggctttaa ggaatattac tgacactggt tttaagttgt tacttttggg attagtaaca
     3781 ttaaactttg ttggtgcatt catgttggaa tctgttcttg atcaatgcct gccagcttgc
     3841 ttgcgtagac ttagaccaaa gagagcgagt aaaaagagat tcaagcaatt agaaagagaa
     3901 ttagcagaac aaccttggcc ccctctacca gctggtccat tgagagcggg tggtggtggt
     3961 agtctagttc cacgtggtag tacggcggct gctcccgggc ctgctcctgc tcctgctcct
     4021 gcctctgcac ccgccgctgc cgccccggcc ggtgccggca ccccggtgac cgccccgctg
     4081 gcgggcacta tctggaaggt gctggccagc gaaggccaga cggtggccgc aggcgaggtg
     4141 ctgctgattc tggaagccat gaagatggaa accgaaatcc gcgccgcgca ggccgggacc
     4201 gtgcgcggta tcgcggtgaa agccggcgac gcggtggcgg tcggcgacac cctgatgacc
     4261 taagagctcg gtacccggat cagcggggga tctcccatgt ctctactggt ggtggtgctt
     4321 ctttggaatt attggaaggt aaggaattgc caggtgttgc tttcttatcc gaaaagaaat
     4381 aaattgaatt gaattgaaat cgatagatca atttttttct tttctctttc cccatccttt
     4441 acgctaaaat aatagtttat tttatttttt gaatattttt tatttatata cgtatatata
     4501 gactattatt tatcttttaa tgattattaa gatttttatt aaaaaaaaat tcgctcctct
     4561 tttaatgcct ttatgcagtt tttttttccc attcgatatt tctatgttcg ggttcagcgt
     4621 attttaagtt taataactcg aaaattctgc gttcgttaaa gcttggcgta atcatggtca
     4681 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4741 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4801 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agagcttcgt aaccgacagt
     4861 ttctaacttt tgtgctttga caagaacttc ttcttcttgc tttaataaaa actgttccat
     4921 tttcgttgta taacttgaat cataagcgcc aagcagtctg acagccaaca gcgcagcgtt
     4981 cgtactatta ttaatagcga cggtagctac tggaacacct ctaggcattt gcacaattga
     5041 atgtaaagaa tctactccat ctagacaaga accttttacg ggcacaccga tgacaggaag
     5101 tggtgtcatt gcagccacca tacctggcaa gtgagcagcc ccaccagctc cagcgataat
     5161 tgttttaatt ccacgcttgc ttgcggaaat agcatatgct gacatcctat gtggagttct
     5221 atgagcagag actattgtca cttcaaatgg aacgccaaaa tcttttaaaa ccgcacatgc
     5281 ggcagacatt accggcaagt cagagtctga tcccatgatg attccaacca atggtttgac
     5341 cattgcttcc aagtccaact tttgagcgac agagattttg attggaatat cagttctacc
     5401 tgtaatgtag ttcagccttt gttcacattc cgccatactg gaggcaataa tatttatgtg
     5461 acctactttt ctgttaggtc tagactcttt tccatataag tacactgagg aacctggagt
     5521 cgccaatgct ctttcgcaag tttctagctc tttatctttt gtatgtttgt ctccaagaac
     5581 atttagcata atggcgttcg ttgtaatggt ggagaaagat gtgaaattct ttggcattgg
     5641 caaatccaat attgatctca aatgagcttc aaattgagaa gtgacgcaag catcaatggt
     5701 ataatgtcca gagttgtgag gccttggggc aatttcgtta ataagcaatt cccctgtttc
     5761 taaatagaac atttccacac caaatatacc acaaccggga aaagatttga ttgcattttc
     5821 tgccaacaac ttcgccttaa gttgaacgga gtccggaact ctagcaggcg cataacataa
     5881 gtcacaaata ttgtccttgt ggatagtctc tacaattggg taagaaaaca ctaaaccgtt
     5941 aacagatctc acaatcatga ctgctaattc tttagtaaat ggtgcccatt tttcggcgta
     6001 caaaggacga tccttcagta cttccaaagc ttccggaatc atttccttat tctttacaac
     6061 gaagttacct cttccatcgt atgccaaagt cctcgacttc aagacgaatg gaaaacccaa
     6121 atctcttcca acattcaata gggacgtctc actggcttgt tccacaggaa cactttgggt
     6181 aactgctata ccatttttga ttaaatgctc tttttgaata tatttgtctt gtatcaatct
     6241 gattgtttct ggagaagggt aaatttttaa tttgggatgt tttacttgaa gattctttag
     6301 tgtaggaaca tcaacatgct caatctcaat cgttagcaca tcacattttt cagctagttt
     6361 ttcgatatca agaggattgg aaaaggagcc attaacgtgg tcattggagt tgcttatttg
     6421 tttggcagga gaattttcag catctagtat taccgtctta atgttgagcc tgtttgctgc
     6481 ctcaacaatc atacgtccca attgtccccc tcctaatata ccaactgttc tagaatccat
     6541 acttgattgt tttgtccgat tttcttgttt ttcttgattg ttatagtagg atgtacttag
     6601 aagagagatc caacgatttt acgcaccaat ttatacatga aatgctccat aatattgtcc
     6661 atttagttct taataaaagg tcagcaagag tcaatcactt agtattaccc ggttcgtagc
     6721 catgcaacaa gagtcatttg tcagcatagc tgtaataatc aatcatgacg taagaaatgt
     6781 atcataatta aaagttgtta aagatgtcag tgttatgttg gtgttacaaa attctcggct
     6841 tctcactaat atttaatatc tcttaaattt tatctgtctt tgattctttt aagaaaagtt
     6901 atgtattatt caagaaaaag tcaattccgc atcaaaaggt aaaatttata taaactgctt
     6961 taaaatttca tgaaactagg caacttttcg aaatgatctt tttcgagcat gaagtttctt
     7021 ttataataac ctggtcaaaa gctctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7081 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7921 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7981 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8041 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8101 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8161 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8221 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8281 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8341 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8401 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8461 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8521 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8581 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8641 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8701 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8761 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8821 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8881 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8941 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9001 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     9061 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     9121 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     9181 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     9241 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     9301 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     9361 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     9421 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     9481 aaacgacggc cagtgaatta attccacgga ctatagacta tactagtata ctccgtctac
     9541 tgtacgatac acttccgctc aggtccttgt cctttaacga ggccttacca ctcttttgtt
     9601 actctattga tccagctcag caaaggcagt gtgatctaag attctatctt cgcgatgtag
     9661 taaaactagc tagaccgaga aagagactag aaatgcaaaa ggcacttcta caatggctgc
     9721 catcattatt atccgatgtg acgctgcagc ttctcaatga tattcgaata cgctttgagg
     9781 agatacagcc taatatccga caaactgttt tacagattta cgatcgtact tgttacccat
     9841 cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
     9901 ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
     9961 gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
    10021 tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
    10081 ttgtagaaca aaaatgcaac gcgagaacgc taatttttca aacaaagaat ctgagctgca
    10141 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
    10201 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
    10261 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
    10321 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
    10381 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
    10441 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
    10501 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
    10561 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
    10621 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
    10681 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
    10741 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
    10801 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
    10861 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
    10921 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
    10981 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
    11041 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
    11101 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
    11161 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta
    11221 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc
    11281 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct
    11341 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat
    11401 catatgcata gtaccgagaa actagtgcga agtagtgatc aggtattgct gttatctgat
    11461 gagtatacgt tgtcctggcc acggcagaag cacgcttatc gctccaattt cccacaacat
    11521 tagtcaactc cgttaggccc ttcattgaaa gaaatgaggt catcaaatgt cttccaatgt
    11581 gagattttgg gccatttttt atagcaaaga ttgaataagg cgcatttttc ttcaaagctt
    11641 tattgtacga tctgactaag ttatctttta ataattggta ttcctgttta ttgcttgaag
    11701 aattgccggt cctatttact cgttttagga ctggttcaga attaattctc atgtttgaca
    11761 gcttatcatc gcgataagct tttcaattca tcattttttt tttattcttt tttttgattt
    11821 cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag
    11881 gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc
    11941 ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg
    12001 tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta
    12061 tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc
    12121 aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat
    12181 gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc
    12241 gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc
    12301 aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca
    12361 cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca
    12421 aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact
    12481 ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc
    12541 tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca
    12601 cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat
    12661 gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga
    12721 agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga
    12781 agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca
    12841 caaattagag cttcaattta attatatcag ttattacccg atcaaaaatc atcgcttcgc
    12901 tgattaatta ccccagaaat aaggctaaaa aactaatcgc attatcatcc tatggttgtt
    12961 aatttgattc gttcatttga aggtttgtgg ggccaggtta ctgccaattt ttcctcttca
    13021 taaccataaa agctagtatt gtagaatctt tattgttcgg agcagtgcgg cgcgaggcac
    13081 atctgcgtt
//
