LOCUS       sequence-34294-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW FLIPglu-600u-Delta-13V
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     187..206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    268..292
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(307..558)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(349..370)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     698..717
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actatttata ataaaaatca taaatcataa gaaattcgct tatttagaag tgtcaacaac
       61 gtatctacca acgatttgac ccttttccat cttttcgtaa atttctggca aggtagacaa
      121 gccgacaacc ttgattggag acttgaccaa acctctggcg aagaagtcca aagctggatc
      181 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
      241 ctgcagcggc catcatgaat taactacacc actttgtaca agaaagctgg gtctagatag
      301 cttttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      361 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
      421 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      481 gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat
      541 gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg ctgttgtagt tgtactccag
      601 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      661 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      721 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      781 ctgcttcatg t
//
