LOCUS       sequence-342965-        6202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pOPIN-GG expression vector compatible with C-terminal tagging, 
            carbenicillin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6202)
  TITLE     Banfield lab plasmids
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPGC-C
FEATURES             Location/Qualifiers
     source          1..6202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     195..1022
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             195..319
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(357..962)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1154..1457
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1983..2005
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2067..2086
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2128..2147
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2128..2146
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2147..2171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2187..2296
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    2426..2447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2462..2467,2468..2485,2486..2492)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2500..2516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2534..2545
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2552..3229
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3274..3345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3361..3388
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3511..3530)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3576..3631
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3630..3649)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      3719..3766
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     3779..4484
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3867..4239
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3896..4484)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(4493..4526)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4694..5281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4773..4792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5294..6085,6086..6154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5917..5936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catgtccgcg cgtttcctgc atcttttaat caaatcccaa gatgtgtata aacgcgccgg
       61 tatgtacagg aagaggttta tactaaactg ttacattgca aacgtggttt cgtgtgccaa
      121 gtgtgaaaac cgatgtttaa tcaaggctct gacgcatttc tacaaccacg actccaagtg
      181 tgtgggtgaa gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg
      241 ccaaaaaatg aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggactcaa
      301 tcctatttgt aattattgaa taataaaaca attataaatg tcaaatttgt tttttattaa
      361 cgatacaaac caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag
      421 ttataatcgc tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac
      481 aatataattt tattttcaca taaactagac gccttgtcgt cctcttcttc gtattccttc
      541 tctttttcat ttttctcttc ataaaaatta acatagttat tatcgtatcc atatatgtat
      601 ctatcgtata gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt
      661 atagtaccgc tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct
      721 tcggagtgtg ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt
      781 ttgtacaata tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt
      841 agcagcaccg gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt
      901 tcacctccct tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc
      961 attgtaatga gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg
     1021 ctgatggccg gccgtaatga gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa
     1081 tatatagttg ctctagttat taatagtaat caattacggg gtcattagtt catagcccat
     1141 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     1201 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     1261 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     1321 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     1381 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1441 tcatcgctat taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     1501 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     1561 atgggggcgg gggggggggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
     1621 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     1681 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     1741 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     1801 agcgggcggg acggcccttc tccttcgggc tgtaattagc gcttggttta atgacggctt
     1861 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     1921 gggagcggct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     1981 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     2041 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     2101 tggcaaagaa ttggatcgga ccgaaattaa tacgactcac tataggggaa ttgtgagcgg
     2161 ataacaattc cccggagtta atccgggacc tttaattcaa cccaacacaa tatattatag
     2221 ttaaataaga attattatca aatcatttgt atattaatta aaatactata ctgtaaatta
     2281 cattttattt acaatcaaag gagatataca atgtgagacc gatatcaata cgcaaaccgc
     2341 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2401 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2461 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2521 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaggacg ttatcaaaga
     2581 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
     2641 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
     2701 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
     2761 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
     2821 tttcaaatgg gaacgtgtta tgaacttcga ggacggtggt gttgttaccg ttacccagga
     2881 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
     2941 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
     3001 gtacccggag gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
     3061 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
     3121 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgagg actacaccat
     3181 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata
     3241 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     3301 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     3361 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc agctttctag ggatccacca
     3421 gtttgtgatt aacctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     3481 gccctggctc acaaatacca ctgagatcga tctttttccc tctgccaaaa attatgggga
     3541 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     3601 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     3661 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact
     3721 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg
     3781 aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac
     3841 ctctgttcac tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag
     3901 cgctagattc tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat
     3961 taaatttata atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt
     4021 tatatctgaa tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt
     4081 caaacaaggg ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg
     4141 ccacaaaact tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt
     4201 tgttttgtaa taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat
     4261 cactgtcgtt agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt
     4321 taacatcggg cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt
     4381 tagaagttgc ttccgaagat gattttgcca tagccacacg acgcctatta attgtgtcgg
     4441 ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc
     4501 gtatagcata cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc
     4561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4681 cgttgctggc gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc
     4741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5101 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5221 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5281 agaagatcct ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5341 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5401 taccatctgg ccccagtgct gcaatgatac cgcgagatcc acgctcaccg gctccagatt
     5461 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5521 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5581 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5641 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5701 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5761 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5821 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5881 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5941 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6001 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6061 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6121 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6181 gcatttatca gggttattgt ct
//
