LOCUS       sequence-34299-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW AT1.03
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..205
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        186..204
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    267..291
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(329..841,842..844,845..847))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     522..541
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     781..802
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttatttata ataaaatcat aaatcataag aaattcgctt atttagaagt gtcaacaacg
       61 tatctaccaa cgatttgacc cttttccatc ttttcgtaaa tttctggcaa ggtagacaag
      121 ccgacaacct tgattggaga cttgaccaaa cctctggcga agaagtccaa agctggatcc
      181 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacc
      241 tgcagcggcc atcatgaatt aactacacca ctttgtacaa gaaagctggg tgcagtcgag
      301 gctgatcagc ggtttaaact taagcttact cgatgttgtg gcggatcttg aagttggcct
      361 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact
      421 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      481 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      541 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      601 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag cccagggtgg
      661 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca
      721 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      781 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
      841 tcaccatgcc accgctgcca cccttgtaca gctcgtccat gccgagagtg atcccggcgg
      901 cggt
//
