LOCUS       sequence-34301-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW AT1.03YEMK
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     187..206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    268..292
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(330..842,843..845,846..848))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     523..542
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     782..803
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(906..927)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acttatttat aataaaatca taaatcataa gaaattcgct tatttagaag tgtcaacaac
       61 gtatctacca acgatttgac ccttttccat cttttcgtaa atttctggca aggtagacaa
      121 gccgacaacc ttgattggag acttgaccaa acctctggcg aagaagtcca aagctggatc
      181 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
      241 ctgcagcggc catcatgaat taactacacc actttgtaca agaaagctgg gtgcagtcga
      301 ggctgatcag cggtttaaac ttaagcttac tcgatgttgt ggcggatctt gaagttggcc
      361 ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgtt gtagttgtac
      421 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      481 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      541 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      601 tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gcccagggtg
      661 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatcag cttcagggtc
      721 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
      781 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
      841 ctcaccatgc caccgctgcc acccttgtac agctcgtcca tgccgagagt gatcccggcg
      901 gcggtcacga actccagcag gaccatgtga tcgcgctttc tcgttggggt ctttgctcag
      961 cttggactgg taactca
//
