LOCUS       sequence-343045-       10589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding GNAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: GNAS_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1001
                     /label=chicken beta-actin promoter
     intron          1002..2010
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1934..1956
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2018..2037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2126..2150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3393..3417)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3437..3460
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3495..4044
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3639..3656)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3866..3885
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4047..4049,4050..4052,4053..4766)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4047..4049,4050..4052,4053..4763)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4092..4113)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4353..4372)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4700..4721
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4842..5430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4895..4915)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5313..5324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5433..5449)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5434..5450)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5639..5872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5944..6065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5981..6000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6035..6054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6105..6240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6260..6279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6261..6279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6302..6660)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6342..6363)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6554..6573
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6687..6791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6792..6860,6861..7652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7010..7029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7823..8411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8312..8331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8586..8889
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8891..9089
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9044..9064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9107..9287
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9334..9459
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9958..10191
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10376..10420
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1021 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1081 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1141 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1201 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1261 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1321 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1441 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1501 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1561 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1621 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1681 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1741 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     1801 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1861 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     1921 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1981 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     2041 tctcatcatt ttggcaaaga attcccctag ccgcggaatc tagactgccg gatcgaattg
     2101 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagcaggct ccgcggccgc
     2161 ccccttcacc atgggctgcc tcgggaacag taagaccgag gaccagcgca acgaggagaa
     2221 ggcgcagcgt gaggccaaca aaaagatcga gaagcagctg cagaaggaca agcaggtcta
     2281 ccgggccacg caccgcctgc tgctgctggg tgctggagaa tctggtaaaa gcaccattgt
     2341 gaagcagatg aggatcctgc atgttaatgg gtttaatgga gagggcggcg aagaggaccc
     2401 gcaggctgca aggagcaaca gcgatggcag tgagaaggca accaaagtgc aggacatcaa
     2461 aaacaacctg aaagaggcga ttgaaaccat tgtggccgcc atgagcaacc tggtgccccc
     2521 cgtggagctg gccaaccccg agaaccagtt cagagtggac tacatcctga gtgtgatgaa
     2581 cgtgcctgac tttgacttcc ctcccgaatt ctatgagcat gccaaggctc tgtgggagga
     2641 tgaaggagtg cgtgcctgct acgaacgctc caacgagtac cagctgattg actgtgccca
     2701 gtacttcctg gacaagatcg acgtgatcaa gcaggctgac tatgtgccga gcgatcagga
     2761 cctgcttcgc tgccgtgtcc tgacttctgg aatctttgag accaagttcc aggtggacaa
     2821 agtcaacttc cacatgtttg acgtgggtgg ccagcgcgat gaacgccgca agtggatcca
     2881 gtgcttcaac gatgtgactg ccatcatctt cgtggtggcc agcagcagct acaacatggt
     2941 catccgggag gacaaccaga ccaaccgcct gcaggaggct ctgaacctct tcaagagcat
     3001 ctggaacaac agatggctgc gcaccatctc tgtgatcctg ttcctcaaca agcaagatct
     3061 gctcgctgag aaagtccttg ctgggaaatc gaagattgag gactactttc cagaatttgc
     3121 tcgctacact actcctgagg atgctactcc cgagcccgga gaggacccac gcgtgacccg
     3181 ggccaagtac ttcattcgag atgagtttct gaggatcagc actgccagtg gagatgggcg
     3241 tcactactgc taccctcatt tcacctgcgc tgtggacact gagaacatcc gccgtgtgtt
     3301 caacgactgc cgtgacatca ttcagcgcat gcaccttcgt cagtacgagc tgctctacta
     3361 gccaactttc ttgtacaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggtggg
     3421 aggaggtgga tctggcgact acaaggacga cgatgacaag tgactcgagc gggatcaatt
     3481 ccgccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     3541 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     3601 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     3661 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     3721 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3781 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3841 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3901 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     3961 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4021 tttcctttga aaaacacgat aataccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4081 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4141 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4201 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4261 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4321 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4381 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4441 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4501 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4561 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4621 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4681 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4741 tcggcatgga cgagctgtac aagtaagcgg ccgcgactct agagtcgacc tgcaggcatg
     4801 caagcttcag gtagccggct aacgttatca agcttatcga taatcaacct ctggattaca
     4861 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4921 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4981 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5041 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5101 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5161 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5221 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5281 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5341 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5401 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta
     5461 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     5521 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     5581 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5641 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     5701 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5761 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5821 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     5881 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     5941 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6061 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6121 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6181 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6241 tagggacgta cccaattcgc cctatagtga gtcgtattag catgtgagca aaaggccagc
     6301 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6361 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6421 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6481 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6541 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6601 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6661 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6721 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6781 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6841 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6901 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6961 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7021 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7081 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7141 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7201 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7261 taactcgcct tgatcgttgg gaaccgaagc tgaatgaagc cataccaaac gacgagcgtg
     7321 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7381 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7441 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7501 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7561 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7621 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7681 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7741 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7801 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7861 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7921 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7981 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8041 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8101 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8161 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8221 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8281 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8341 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8401 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8461 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     8521 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     8581 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     8641 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     8701 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     8761 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     8821 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     8881 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     8941 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     9001 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     9061 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     9121 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9181 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9241 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     9301 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9361 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     9421 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     9481 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     9541 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9601 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9661 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9721 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9781 accaccgcac agcaagcggc cggccgcgct gatcttcaga cctggaggag gagatatgag
     9841 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     9901 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     9961 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10021 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    10081 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    10141 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    10201 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    10261 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    10321 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    10381 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    10441 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    10501 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    10561 gtttcagacc cacctcccaa ccccgaggg
//
