LOCUS       sequence-343046-       12671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding GLI1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12671)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 12671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: GLI1_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..12671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1001
                     /label=chicken beta-actin promoter
     intron          1002..2010
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1934..1956
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2018..2037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2126..2150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5475..5499)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5519..5542
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5577..6126
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5721..5738)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5948..5967
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6129..6131,6132..6134,6135..6848)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6129..6131,6132..6134,6135..6845)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6174..6195)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6435..6454)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6782..6803
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6924..7512
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6977..6997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7395..7406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7515..7531)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7516..7532)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7721..7954
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8026..8147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8063..8082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8117..8136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8187..8322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8342..8361)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8343..8361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(8384..8742)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8424..8445)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8636..8655
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8769..8873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8874..8942,8943..9734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9092..9111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9905..10493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10394..10413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        10668..10971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10973..11171
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11126..11146
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11189..11369
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11416..11541
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12040..12273
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12458..12502
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1021 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1081 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1141 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1201 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1261 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1321 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1441 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1501 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1561 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1621 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1681 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1741 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     1801 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1861 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     1921 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1981 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     2041 tctcatcatt ttggcaaaga attcccctag ccgcggaatc tagactgccg gatcgaattg
     2101 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagttggca ccatgttcaa
     2161 ctcgatgacc ccaccaccaa tcagtagcta tggcgagccc tgctgtctcc ggcccctccc
     2221 cagtcagggg gcccccagtg tggggacaga aggactgtct ggcccgccct tctgccacca
     2281 agctaacctc atgtccggcc cccacagtta tgggccagcc agagagacca acagctgcac
     2341 cgagggccca ctcttttctt ctccccggag tgcagtcaag ttgaccaaga agcgggcact
     2401 gtccatctca cctctgtcgg atgccagcct ggacctgcag acggttatcc gcacctcacc
     2461 cagctccctc gtagctttca tcaactcgcg atgcacatct ccaggaggct cctacggtca
     2521 tctctccatt ggcaccatga gcccatctct gggattccca gcccagatga atcaccaaaa
     2581 agggccctcg ccttcctttg gggtccagcc ttgtggtccc catgactctg cccggggtgg
     2641 gatgatccca catcctcagt cccggggacc cttcccaact tgccagctga agtctgagct
     2701 ggacatgctg gttggcaagt gccgggagga acccttggaa ggtgatatgt ccagccccaa
     2761 ctccacaggc atacaggatc ccctgttggg gatgctggat gggcgggagg acctcgagag
     2821 agaggagaag cgtgagcctg aatctgtgta tgaaactgac tgccgttggg atggctgcag
     2881 ccaggaattt gactcccaag agcagctggt gcaccacatc aacagcgagc acatccacgg
     2941 ggagcggaag gagttcgtgt gccactgggg gggctgctcc agggagctga ggcccttcaa
     3001 agcccagtac atgctggtgg ttcacatgcg cagacacact ggcgagaagc cacacaagtg
     3061 cacgtttgaa gggtgccgga agtcatactc acgcctcgaa aacctgaaga cgcacctgcg
     3121 gtcacacacg ggtgagaagc catacatgtg tgagcacgag ggctgcagta aagccttcag
     3181 caatgccagt gaccgagcca agcaccagaa tcggacccat tccaatgaga agccgtatgt
     3241 atgtaagctc cctggctgca ccaaacgcta tacagatcct agctcgctgc gaaaacatgt
     3301 caagacagtg catggtcctg acgcccatgt gaccaaacgg caccgtgggg atggccccct
     3361 gcctcgggca ccatccattt ctacagtgga gcccaagagg gagcgggaag gaggtcccat
     3421 cagggaggaa agcagactga ctgtgccaga gggtgccatg aagccacagc caagccctgg
     3481 ggcccagtca tcctgcagca gtgaccactc cccggcaggg agtgcagcca atacagacag
     3541 tggtgtggaa atgactggca atgcaggggg cagcactgaa gacctctcca gcttggacga
     3601 gggaccttgc attgctggca ctggtctgtc cactcttcgc cgccttgaga acctcaggct
     3661 ggaccagcta catcaactcc ggccaatagg gacccggggt ctcaaactgc ccagcttgtc
     3721 ccacaccggt accactgtgt cccgccgcgt gggcccccca gtctctcttg aacgccgcag
     3781 cagcagctcc agcagcatca gctctgccta tactgtcagc cgccgctcct ccctggcctc
     3841 tcctttcccc cctggctccc caccagagaa tggagcatcc tccctgcctg gccttatgcc
     3901 tgcccagcac tacctgcttc gggcaagata tgcttcagcc agagggggtg gtacttcgcc
     3961 cactgcagca tccagcctgg atcggatagg tggtcttccc atgcctcctt ggagaagccg
     4021 agccgagtat ccaggataca accccaatgc aggggtcacc cggagggcca gtgacccagc
     4081 ccaggctgct gaccgtcctg ctccagctag agtccagagg ttcaagagcc tgggctgtgt
     4141 ccatacccca cccactgtgg cagggggagg acagaacttt gatccttacc tcccaacctc
     4201 tgtctactca ccacagcccc ccagcatcac tgagaatgct gccatggatg ctagagggct
     4261 acaggaagag ccagaagttg ggacctccat ggtgggcagt ggtctgaacc cctatatgga
     4321 cttcccacct actgatactc tgggatatgg gggacctgaa ggggcagcag ctgagcctta
     4381 tggagcgagg ggtccaggct ctctgcctct tgggcctggt ccacccacca actatggccc
     4441 caacccctgt ccccagcagg cctcatatcc tgaccccacc caagaaacat ggggtgagtt
     4501 cccttcccac tctgggctgt acccaggccc caaggctcta ggtggaacct acagccagtg
     4561 tcctcgactt gaacattatg gacaagtgca agtcaagcca gaacaggggt gcccagtggg
     4621 gtctgactcc acaggactgg caccctgcct caatgcccac cccagtgagg ggcccccaca
     4681 tccacagcct ctcttttccc attaccccca gccctctcct ccccaatatc tccagtcagg
     4741 cccctatacc cagccacccc ctgattatct tccttcagaa cccaggcctt gcctggactt
     4801 tgattccccc acccattcca cagggcagct caaggctcag cttgtgtgta attatgttca
     4861 atctcaacag gagctactgt gggagggtgg gggcagggaa gatgcccccg cccaggaacc
     4921 ttcctaccag agtcccaagt ttctgggggg ttcccaggtt agcccaagcc gtgctaaagc
     4981 tccagtgaac acatatggac ctggctttgg acccaacttg cccaatcaca agtcaggttc
     5041 ctatcccacc ccttcaccat gccatgaaaa ttttgtagtg ggggcaaata gggcttcaca
     5101 tagggcagca gcaccacctc gacttctgcc cccattgccc acttgctatg ggcctctcaa
     5161 agtgggaggc acaaacccca gctgtggtca tcctgaggtg ggcaggctag gagggggtcc
     5221 tgccttgtac cctcctcccg aaggacaggt atgtaacccc ctggactctc ttgatcttga
     5281 caacactcag ctggactttg tggctattct ggatgagccc caggggctga gtcctcctcc
     5341 ttcccatgat cagcggggca gctctggaca taccccacct ccctctgggc cccccaacat
     5401 ggctgtgggc aacatgagtg tcttactgag atccctacct ggggaaacag aattcctcaa
     5461 ctctagtgcc taattgccaa ctttcttgta caaagtggtg ggaggaggtg gatctggcga
     5521 ctacaaggac gacgatgaca agtgactcga gcgggatcaa ttccgccccc cccctaacgt
     5581 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5641 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5701 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5761 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5821 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5881 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5941 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     6001 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     6061 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     6121 ataataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     6181 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     6241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     6301 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     6361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     6421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     6481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     6541 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     6601 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     6661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     6721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     6781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     6841 acaagtaagc ggccgcgact ctagagtcga cctgcaggca tgcaagcttc aggtagccgg
     6901 ctaacgttat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     6961 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7021 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7081 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7141 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7201 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7261 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7321 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7381 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7441 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7501 ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc
     7561 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7621 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7741 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7801 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7861 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7921 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7981 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8041 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8101 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8161 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8221 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8281 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     8341 gccctatagt gagtcgtatt agcatgtgag caaaaggcca gcataaatca aaagaataga
     8401 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8521 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8701 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     8761 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8821 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8881 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8941 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9001 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9061 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9121 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9181 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9241 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9301 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9361 gggaaccgaa gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9421 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9481 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9541 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9601 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9661 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9721 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9781 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9841 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9901 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9961 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10021 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
    10081 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10141 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10201 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10261 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10321 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10381 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10441 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10501 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10561 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag
    10621 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    10681 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    10741 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    10801 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    10861 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    10921 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    10981 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    11041 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    11101 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    11161 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    11221 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    11281 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    11341 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    11401 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    11461 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    11521 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    11581 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    11641 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    11701 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    11761 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    11821 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    11881 gccggccgcg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    11941 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    12001 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    12061 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    12121 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    12181 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    12241 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    12301 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    12361 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    12421 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    12481 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    12541 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    12601 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    12661 aaccccgagg g
//
