LOCUS       sequence-343047-       10859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding Sufu.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: Sufu_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1001
                     /label=chicken beta-actin promoter
     intron          1002..2010
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1934..1956
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2018..2037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2126..2150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3663..3687)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3707..3730
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3765..4314
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3909..3926)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4136..4155
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4317..4319,4320..4322,4323..5036)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4317..4319,4320..4322,4323..5033)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4362..4383)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4623..4642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4970..4991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5112..5700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5165..5185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5583..5594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5703..5719)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5704..5720)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5909..6142
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6214..6335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6251..6270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6305..6324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6375..6510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6530..6549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6531..6549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6572..6930)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6612..6633)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6824..6843
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6957..7061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7062..7130,7131..7922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7280..7299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8093..8681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8582..8601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8856..9159
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9161..9359
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9314..9334
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9377..9557
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9604..9729
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10228..10461
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10646..10690
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1021 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1081 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1141 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1201 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1261 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1321 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1441 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1501 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1561 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1621 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1681 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1741 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     1801 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1861 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     1921 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1981 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     2041 tctcatcatt ttggcaaaga attcccctag ccgcggaatc tagactgccg gatcgaattg
     2101 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagcaggct ccgcggccgc
     2161 ccccttcacc atggcggagc tgcggcctag cgtcgccccc ggtcccgccg cgcccccggc
     2221 ctctggccct agtgcccctc cggcctttgc ttcactcttt cccccgggac tgcacgccat
     2281 ctacggagag tgtcgccgcc tctaccctga ccagccgaac ccgctccagg ttaccgctat
     2341 cgtcaagtac tggttgggtg gtccggaccc cttggactat gttagcatgt acaggaacat
     2401 ggggagtcct tctgccaaca tccctgagca ctggcactac atcagctttg gcctgagtga
     2461 tctctatggt gacaacagag tccatgagtt tacaggaaca gacggaccaa gtggatttgg
     2521 ctttgagttg acgtttcgtc tgaagagaga aactggggag tctgccccac caacatggcc
     2581 agcagagctg atgcagggcc tagcccgata tgtcttccag tcagagaaca ccttctgtag
     2641 cggggaccat gtgtcttggc acagcccttt ggataacagt gagtcaagaa ttcagcacat
     2701 gctgctgacg gaggacccac agatgcagcc tgtgcggaca ccctttgggg tagtgacttt
     2761 cctccagatt gttggtgtct gcactgagga gttacattca gcccaacagt ggaacgggca
     2821 gggcatcctg gaactactac ggacagtgcc cattgctggc ggtccctggc tgataactga
     2881 catgcggcgg ggagaaacca tatttgagat cgatccgcac ctgcaagaga gagttgacaa
     2941 aggcattgag acagacggtt ctaacctgag cggcgtcagt gccaagtgtg cctgggatga
     3001 cctcagccgg cctccggagg atgaagagga tagccggagc atctgcctcg gcacacagcc
     3061 tcggaggctg tctggcaaag acacagagca gatccgggag accctgaggc ggggactgga
     3121 gattaacagc aaacctgtcc ttccaccaat caattctcag cgacagaacg gcctcaccca
     3181 cgacagggct ccgagccgca aggacagttt gggcagcgac agctccacgg ccatcatccc
     3241 ccacgagctg atccgcacac ggcagctcga gagcgtgcat ctaaaattta accaagagtc
     3301 gggagccctc atccctctct gcctaagggg cagactccta catggccggc acttcaccta
     3361 caagagtatc acaggcgaca tggccatcac gtttgtgtcc acgggagtgg aaggcgcctt
     3421 tgccactgag gaacacccgt atgcagctca cggaccctgg ttacaaattc tgttgacaga
     3481 agagtttgta gagaagatgt tggaggactt agaagatcta acctctccag aggaatttaa
     3541 acttcccaaa gagtacagct ggcctgagaa gaaactcaaa gtgtccattc tccccgacgt
     3601 ggtgttcgac agtccgctac actaggtcga cggtaccgcg ggcccgaagg gtgggcgcgc
     3661 cgacccagct ttcttgtaca aagtggtggg aggaggtgga tctggcgact acaaggacga
     3721 cgatgacaag tgactcgagc gggatcaatt ccgccccccc cctaacgtta ctggccgaag
     3781 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     3841 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     3901 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     3961 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4021 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4081 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4141 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4201 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4261 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg
     4321 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4381 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4441 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4501 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4561 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4621 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4681 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4741 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4801 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4861 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4921 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4981 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg
     5041 ccgcgactct agagtcgacc tgcaggcatg caagcttcag gtagccggct aacgttatca
     5101 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5161 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5221 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5281 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5341 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5401 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5461 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5521 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5581 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5641 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5701 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5761 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5821 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5881 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5941 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6001 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6061 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6121 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6181 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6241 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6301 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6481 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     6541 gtcgtattag catgtgagca aaaggccagc ataaatcaaa agaatagacc gagatagggt
     6601 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     6661 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     6721 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     6781 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     6841 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     6901 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     6961 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7021 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7081 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7141 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7201 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7261 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7321 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7381 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7441 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7501 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccgaagc
     7561 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7621 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7681 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7741 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7801 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7861 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7921 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7981 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8041 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8101 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8161 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8221 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8281 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8341 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8401 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8461 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8521 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8581 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8641 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8701 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8761 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
     8821 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     8881 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     8941 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9001 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9061 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9121 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     9181 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     9241 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     9301 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     9361 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9421 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9481 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9541 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     9601 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     9661 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     9721 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     9781 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     9841 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     9901 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9961 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    10021 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgcgct
    10081 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    10141 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    10201 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    10261 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    10321 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    10381 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
    10441 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
    10501 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
    10561 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    10621 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    10681 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    10741 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    10801 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgaggg
//
