LOCUS       sequence-343058-       10400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding CRYZL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: CRYZL1_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1001
                     /label=chicken beta-actin promoter
     intron          1002..2010
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1934..1956
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2018..2037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2126..2150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3204..3228)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3248..3271
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3306..3855
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3450..3467)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3677..3696
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3858..3860,3861..3863,3864..4577)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3858..3860,3861..3863,3864..4574)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3903..3924)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4164..4183)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4511..4532
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4653..5241
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4706..4726)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5124..5135)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5244..5260)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5245..5261)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5450..5683
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5755..5876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5792..5811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5846..5865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5916..6051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6071..6090)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6072..6090)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6113..6471)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6153..6174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6365..6384
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6498..6602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6603..6671,6672..7463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6821..6840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7634..8222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8123..8142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8397..8700
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8702..8900
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8855..8875
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8918..9098
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9145..9270
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9769..10002
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10187..10231
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1021 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1081 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1141 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1201 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1261 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1321 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1441 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1501 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1561 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1621 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1681 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1741 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     1801 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1861 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     1921 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1981 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     2041 tctcatcatt ttggcaaaga attcccctag ccgcggaatc tagactgccg gatcgaattg
     2101 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagcaggct taatgaaagg
     2161 cttatatttc caacagagtt ccacagatga agaaataaca tttgtatttc aagaaaagga
     2221 agatcttcct gttacagagg ataactttgt gaaacttcaa gttaaagctt gtgctctgag
     2281 ccagataaat acaaagcttc tggcagaaat gaagatgaaa aaggatttat ttcctgttgg
     2341 gagagaaatt gctggaattg tattagatgt tggaagcaag gtatcattct ttcaaccaga
     2401 tgatgaagta gttggaattt tgcccctgga ctctgaagac cctggacttt gtgaagttgt
     2461 tagagtacat gagcattact tggttcataa accagaaaag gtcacatgga cggaagcagc
     2521 aggaagcatt cgggatggag tgcgtgccta tacagctctg cattatcttt ctcatctctc
     2581 tcctggaaaa tcagtgctga taatggatgg agcaagtgca tttggtacaa tagctattca
     2641 gttagcacat catagaggag ccaaagtgat ttcaacagca tgcagccttg aagataagca
     2701 gtgccttgaa agattcagac ctcccatagc ccgagtgatt gatgtatcta atgggaaagt
     2761 tcatgttgct gaaagctgtt tggaagaaac aggtggcctg ggagtagata ttgtcctaga
     2821 tgctggagtg agattatata gtaaagatga tgaaccagct gtaaaactac aactactacc
     2881 acataaacat gatatcatca cacttcttgg tgttggaggc cactgggtaa caacagaaga
     2941 aaaccttcag ttggatcctc cagatagcca ctgccttttc ctcaagggag caacgttagc
     3001 tttcctgaat gatgaagttt ggaatttgtc aaatgtacaa cagggaaaat atctttgtat
     3061 cttaaaggat gtgatggaga agttatcaac tggtgttttc agacctcagt tggatgaacc
     3121 cattccactg tatgaggcaa aagtttccat ggaagctgtt cagaaaaatc aaggaagaaa
     3181 aaagcaagtt gttcaatttt agtacccagc tttcttgtac aaagtggtgg gaggaggtgg
     3241 atctggcgac tacaaggacg acgatgacaa gtgactcgag cgggatcaat tccgcccccc
     3301 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     3361 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     3421 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     3481 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     3541 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     3601 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     3661 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     3721 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     3781 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     3841 aaaaacacga taataccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3901 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3961 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4021 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4081 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4141 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4201 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4261 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4321 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4381 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4441 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     4501 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4561 acgagctgta caagtaagcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttca
     4621 ggtagccggc taacgttatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     4681 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4741 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4801 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4861 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4921 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4981 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5041 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5101 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5161 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5221 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     5281 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     5341 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     5401 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5461 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5521 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5581 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5641 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5701 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     5761 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5821 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5881 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5941 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6001 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6061 acccaattcg ccctatagtg agtcgtatta gcatgtgagc aaaaggccag cataaatcaa
     6121 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     6181 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     6241 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     6301 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     6361 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     6421 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     6481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6841 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7081 ttgatcgttg ggaaccgaag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7741 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8281 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
     8341 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     8401 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     8461 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     8521 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     8581 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8641 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8701 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     8761 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     8821 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     8881 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     8941 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9001 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9061 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9121 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9181 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9241 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9301 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9361 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9421 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9481 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9541 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9601 cagcaagcgg ccggccgcgc tgatcttcag acctggagga ggagatatga gggacaattg
     9661 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9721 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9781 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9841 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9901 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9961 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    10021 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    10081 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    10141 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    10201 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    10261 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    10321 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    10381 ccacctccca accccgaggg
//
