LOCUS       sequence-343063-       11030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding CHAF1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: CHAF1B_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1001
                     /label=chicken beta-actin promoter
     intron          1002..2010
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1934..1956
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2018..2037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2126..2150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3834..3858)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3878..3901
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3936..4485
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4080..4097)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4307..4326
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4488..4490,4491..4493,4494..5207)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4488..4490,4491..4493,4494..5204)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4533..4554)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4794..4813)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5141..5162
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5283..5871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5336..5356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5754..5765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5874..5890)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5875..5891)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6080..6313
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6385..6506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6422..6441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6476..6495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6546..6681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6701..6720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6702..6720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6743..7101)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6783..6804)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6995..7014
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7128..7232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7233..7301,7302..8093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7451..7470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8264..8852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8753..8772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9027..9330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9332..9530
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9485..9505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9548..9728
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9775..9900
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10399..10632
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10817..10861
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1021 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1081 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1141 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1201 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1261 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1321 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1441 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1501 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1561 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1621 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1681 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1741 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     1801 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1861 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     1921 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1981 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     2041 tctcatcatt ttggcaaaga attcccctag ccgcggaatc tagactgccg gatcgaattg
     2101 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagcaggct taatgaaagt
     2161 catcacttgt gaaatagcct ggcacaacaa ggagcccgtg tacagcctgg acttccagca
     2221 tgggacggct gggaggatcc acagactggc gtctgccggc gtggacacca atgtcaggat
     2281 ctggaaggta gaaaagggac cagatggaaa agccatcgtg gaatttttgt ccaatcttgc
     2341 tcgtcatacc aaagccgtca atgttgtgcg tttttctcca actggggaaa ttttagcatc
     2401 gggaggagat gatgctgtca tcctattgtg gaaggtgaat gataacaagg agccggagca
     2461 gatcgctttt caggatgagg acgaggccca gctgaacaag gagaactgga cggttgtgaa
     2521 gactctgcgg ggccacttag aagatgtgta tgatatttgc tgggcaactg atgggaattt
     2581 aatggcttct gcctctgtgg ataacacagc catcatatgg gatgtcagca aaggacaaaa
     2641 gatatcaatt tttaatgaac ataaaagtta tgtccaagga gtaacctggg accctttggg
     2701 tcaatatgtt gctactctga gctgtgacag ggtgctgcga gtatacagta tacagaagaa
     2761 gcgtgtggct ttcaatgttt cgaagatgct gtctggaata ggggctgaag gagaggcaag
     2821 aagctaccgg atgtttcacg acgacagcat gaagtctttc ttccgtagac tgagtttcac
     2881 tcccgacgga tctttgcttc tcacgccagc tggatgtgtg gaatctggtg aaaatgtaat
     2941 gaataccact tatgttttct ccaggaagaa tcttaaaagg cccatcgctc atcttccatg
     3001 tcctggaaaa gccactcttg ctgttcgctg ctgtccggtc tactttgaac tgaggccagt
     3061 ggtggaaacg ggtgtggagc tgatgagtct gccctaccgc ctggtgtttg ctgtggcctc
     3121 ggaggattcc gtgcttctgt atgacaccca gcagtccttc ccttttggtt acgtgtctaa
     3181 tatacattac cacaccctca gtgacatttc atggtccagc gatggtgcct tcctggccat
     3241 ttcttccacg gacggttact gctcatttgt gacatttgag aaagatgaac ttggaattcc
     3301 tttgaaagag aagccagttt tgaacatgag aactcctgat acagcaaaga aaaccaagag
     3361 tcagacacat cgagggtctt cgccaggacc cagaccggta gagggaaccc ctgccagcag
     3421 aacccaagac cccagcagcc ccggcacgac tccccctcag gccagacagg ccccagcccc
     3481 aacagtcatc agggaccctc cctccatcac tcctgctgtc aaaagcccct tgccggggcc
     3541 ttcggaggag aagaccctgc agcccagtag tcaaaacaca aaagcccacc catcccggag
     3601 ggtcactctg aacacactgc aagcctggag caagacaaca ccccggagaa taaacttaac
     3661 acccttaaag acggacactc caccaagttc tgtaccaacc agtgtgattt ccaccccttc
     3721 tacagaagaa attcagtcag agacgcctgg agacgctcag ggcagtcccc cagagctaaa
     3781 gcggcccaga ctcgatgaaa acaaaggagg cacggaaagt ctggaccctt agtacccagc
     3841 tttcttgtac aaagtggtgg gaggaggtgg atctggcgac tacaaggacg acgatgacaa
     3901 gtgactcgag cgggatcaat tccgcccccc ccctaacgtt actggccgaa gccgcttgga
     3961 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     4021 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     4081 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     4141 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     4201 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     4261 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     4321 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     4381 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     4441 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg gtgagcaagg
     4501 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4561 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4621 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4681 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4741 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4801 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4861 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4921 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4981 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     5041 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5101 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5161 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg gccgcgactc
     5221 tagagtcgac ctgcaggcat gcaagcttca ggtagccggc taacgttatc aagcttatcg
     5281 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5341 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5401 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5461 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5521 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5581 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5641 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5701 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5761 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5821 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     5881 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     5941 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     6001 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6061 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     6121 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6181 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6241 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6301 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6361 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6481 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6541 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6601 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6661 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6721 gcatgtgagc aaaaggccag cataaatcaa aagaatagac cgagataggg ttgagtgttg
     6781 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6841 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     6901 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6961 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     7021 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     7081 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7201 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7261 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7321 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7381 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7441 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7501 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7561 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7621 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7681 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccgaag ctgaatgaag
     7741 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7801 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7861 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7921 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7981 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8041 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8401 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8881 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8941 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     9001 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     9061 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     9121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     9181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     9241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     9301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     9361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     9421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     9481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     9541 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9601 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9661 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9721 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     9781 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     9841 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     9901 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     9961 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    10021 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    10081 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    10141 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    10201 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgcgc tgatcttcag
    10261 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10321 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10381 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10441 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10501 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10561 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10621 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10681 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10741 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10801 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10861 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10921 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10981 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
//
