LOCUS       sequence-343064-       10090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding DsRed2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: DsRed2_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1001
                     /label=chicken beta-actin promoter
     intron          1002..2010
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1934..1956
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2018..2037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2126..2150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2871
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2374..2394)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2786..2809
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(2894..2918)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2938..2961
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2996..3545
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3140..3157)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3367..3386
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3548..3550,3551..3553,3554..4267)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3548..3550,3551..3553,3554..4264)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3593..3614)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3854..3873)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4201..4222
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4343..4931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4396..4416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4814..4825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4934..4950)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4935..4951)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5140..5373
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5445..5566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5482..5501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5536..5555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5606..5741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5761..5780)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5762..5780)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5803..6161)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5843..5864)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6055..6074
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6188..6292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6293..6361,6362..7153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6511..6530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7324..7912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7813..7832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8087..8390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8392..8590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8545..8565
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8608..8788
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8835..8960
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9459..9692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9877..9921
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1021 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1081 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1141 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1201 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1261 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1321 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1441 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1501 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1561 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1621 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1681 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1741 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     1801 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1861 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     1921 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1981 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     2041 tctcatcatt ttggcaaaga attcccctag ccgcggaatc tagactgccg gatcgaattg
     2101 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagcaggct ttaaaggaac
     2161 caattcagtc gactggatcc accggtcgcc accatggcct cctccgagaa cgtcatcacc
     2221 gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc
     2281 gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc
     2341 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     2401 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag
     2521 gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc
     2581 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc
     2641 ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac
     2701 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg
     2761 cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc
     2821 atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta gcggccgcac
     2881 tcgagatatc tagacccagc tttcttgtac aaagtggtgg gaggaggtgg atctggcgac
     2941 tacaaggacg acgatgacaa gtgactcgag cgggatcaat tccgcccccc ccctaacgtt
     3001 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     3061 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     3121 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     3181 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     3241 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     3301 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     3361 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     3421 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     3481 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     3541 taataccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3601 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3661 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3721 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3781 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3841 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3901 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3961 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4021 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4081 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4141 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4201 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4261 caagtaagcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttca ggtagccggc
     4321 taacgttatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     4381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     4741 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4861 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4921 cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca
     4981 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     5041 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     5101 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5161 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5221 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5281 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5341 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5401 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5581 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5641 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5701 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5761 ccctatagtg agtcgtatta gcatgtgagc aaaaggccag cataaatcaa aagaatagac
     5821 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     5881 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     5941 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6001 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6061 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6121 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     6181 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6241 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6301 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6361 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6421 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6481 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6541 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6601 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6661 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6721 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6781 ggaaccgaag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6841 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6901 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6961 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7021 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7081 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7141 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7201 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7441 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7561 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7621 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7681 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7741 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7801 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7861 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7921 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7981 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
     8041 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     8101 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8161 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8221 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     8281 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     8341 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     8401 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     8461 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     8521 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     8581 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8641 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8701 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8761 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8821 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8881 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8941 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     9001 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     9061 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     9121 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     9181 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     9241 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     9301 ccggccgcgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9361 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9421 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9481 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9541 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9601 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9661 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9721 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9781 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9841 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9901 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9961 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10021 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10081 accccgaggg
//
