LOCUS       sequence-34307-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Chen J, Kastan MB
  TITLE     5'-3'-UTR interactions regulate p53 mRNA translation and provide a 
            target for modulating p53 induction after DNA damage.
  JOURNAL   Genes Dev. 2010 Oct 1. 24(19):2146-56.
  PUBMED    20837656
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Oct 1. 24(19):2146-56."
COMMENT     Sequence Label: 145-pGL3ctrl-3' UTR
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(195..391)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      209..344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    446..537
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(478..497)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    551..599
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(866..887)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatggc agtgacccgg aaggcagtct
       61 ggctgccaat ccagggaagc gtgtcaccgt cgtggaaagc acgctcccag cccgaacgca
      121 aagtgtcccc ggagcccagc agctacctgc tccctggacg gtggctctag acttttgaga
      181 aggaattcaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
      241 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcga
      301 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
      361 gggactatgg ttgctgacta attgagatgc agatcgcaga tctcgagccc gggctagcac
      421 gcgtaagagc tcggtaccta tcgatagaga aatgttctgg cacctgcact tgcactgggg
      481 acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt
      541 actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
      601 cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt agcttgggct
      661 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      721 gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
      781 tcagctcatt ttttaaccaa tanncgaaat cggcaaaatc ccttataaat caaaagaata
      841 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      901 g
//
