LOCUS       sequence-343071-        1302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of APEX2 in the periplasm from a codon-optimized gene 
            (single-copy integrating plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ahamed M, Jaisinghani N, Li M, Winkeler I, Silva S, Previti ML, 
            Seeliger JC
  TITLE     Optimized APEX2 peroxidase-mediated proximity labeling in fast- and 
            slow-growing mycobacteria.
  JOURNAL   Methods Enzymol. 2022;664:267-289. doi: 10.1016/bs.mie.2021.11.021. 
            Epub 2021 Dec 30.
  PUBMED    35331378
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2022"; volume: "664:267-289. doi"; pages: " 
            10.1016/bs.mie.2021.11.021. Epub 2021 Dec 30"
COMMENT     Sequence Label: pRiboI-Sec-APEX2m
FEATURES             Location/Qualifiers
     source          1..1302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      419..428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc
       61 cgcggtacca gatctttaaa tctagaggtg accacaacgc gcccgctttg atcggggacg
      121 tctgcggccg accatttacg ggtcttgttg tcgttggcgg tcatgggccg aacatactca
      181 cccggatcgg agggccgagg acaaggtcga acgaggggca tgacccggtg cggggcttct
      241 tgcactcggc ataggcgagt gctaagaata acgttggcac tcgcgaccgg gatacgactc
      301 actataggta ccggtgatac cagcatcgtc ttgatgccct tggcagcacc ctgctaagga
      361 ggcaacaaga tgcatatgaa gctcaccaca atgatcaaga cggcagtagc ggtcgtggcc
      421 atggcggcca tcgcgacctt tgcggcaccg gtcgcgttgg ctggatccgg caagagctac
      481 ccgaccgtca gcgccgacta ccaggacgcc gtggagaagg ccaagaagaa gctgcgcggc
      541 ttcatcgccg agaagcggtg cgccccgctg atgctgcgcc tggcgttcca cagcgcgggc
      601 accttcgaca agggcaccaa gaccggcggc ccgttcggca ccatcaagca cccggcggag
      661 ctggcgcaca gcgccaacaa cggcctggac atcgcggtcc ggctgctgga gccgctgaag
      721 gcggagttcc cgatcctgag ctacgccgac ttctaccagc tggcgggcgt cgtggccgtc
      781 gaggtgaccg gcggcccgaa ggtgccgttc cacccgggcc gggaggacaa gccggagccg
      841 ccgccggagg gccgcctgcc ggacccgacc aagggctcgg accacctgcg ggacgtcttc
      901 ggcaaggcga tgggcctgac cgaccaggac atcgtggccc tgagcggcgg ccacaccatc
      961 ggcgcggccc acaaggagcg cagcggcttc gagggcccgt ggaccagcaa cccgctgatc
     1021 ttcgacaact cgtacttcac cgagctgctg agcggcgaga aggaaggcct gctgcagctg
     1081 ccgagcgaca aggcgctgct gagcgacccg gtcttccggc ccctggtgga caagtacgcg
     1141 gccgacgagg acgccttctt cgccgactac gccgaggccc accagaagct gtccgagctg
     1201 ggcttcgccg acgccaagct ttgaatcgat gtcgacgtag ttaactagcg tacgatcgac
     1261 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tg
//
