LOCUS       sequence-343074-        2767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expression under the control of E. coli dnaK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Saltepe B, Bozkurt EU, Haciosmanoglu N, Seker UOS
  TITLE     Genetic Circuits To Detect Nanomaterial Triggered Toxicity through 
            Engineered Heat Shock Response Mechanism.
  JOURNAL   ACS Synth Biol. 2019 Oct 18;8(10):2404-2417. doi: 
            10.1021/acssynbio.9b00291. Epub 2019 Oct 2.
  PUBMED    31536326
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00291"; journalName: "ACS Synth Biol"; date: 
            "2019-10-18- 18"; volume: "8"; issue: "10"; pages: "2404-2417"
COMMENT     Sequence Label: PdnaK GFP pZa
FEATURES             Location/Qualifiers
     source          1..2767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      11..97
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(925..1019)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1040..1699)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1614..1633
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1700..1802)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     RBS             2020..2031
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2044..2757
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2074..2102)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2697..2717
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgcgtggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
       61 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
      841 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
      901 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
      961 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1021 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1081 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1141 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1201 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1261 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1321 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1381 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1441 tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca
     1501 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1561 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1621 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1681 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1741 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     1801 caacgtctca ttttcgccag atatcgacgt cctcgagaaa agcacaaaaa atttttgcat
     1861 ctcccccttg atgacgtggt ttacgacccc atttagtagt caaccgcagt gagtgagtct
     1921 gcaaaaaaat gaaattgggc agttgaaacc agacgtttcg cccctattac agactcacaa
     1981 ccacatgatg accgaatata tagtggagac gtttagatga aagaggagaa aacgcgtggt
     2041 accatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     2101 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     2161 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     2221 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     2281 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     2341 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     2401 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     2461 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     2521 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     2581 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     2641 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     2701 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     2761 aagctta
//
