LOCUS       sequence-343078-        2744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expression under the control of E. coli ibpA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Saltepe B, Bozkurt EU, Haciosmanoglu N, Seker UOS
  TITLE     Genetic Circuits To Detect Nanomaterial Triggered Toxicity through 
            Engineered Heat Shock Response Mechanism.
  JOURNAL   ACS Synth Biol. 2019 Oct 18;8(10):2404-2417. doi: 
            10.1021/acssynbio.9b00291. Epub 2019 Oct 2.
  PUBMED    31536326
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00291"; journalName: "ACS Synth Biol"; date: 
            "2019-10-18- 18"; volume: "8"; issue: "10"; pages: "2404-2417"
COMMENT     Sequence Label: PibpA GFP pZa
FEATURES             Location/Qualifiers
     source          1..2744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(112..657)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(192..211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(771..865)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(886..1545)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1460..1479
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1546..1648)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1867..2580
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1897..1925)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2520..2540
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2601..2687
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2722..2739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
       61 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
      121 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
      181 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
      241 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
      301 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
      361 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
      421 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
      481 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
      541 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
      601 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
      661 gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat
      721 gtagcacctg aagtcagccc catacgatat aagttgttac tagtgcttgg attctcacca
      781 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      841 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      901 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      961 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1021 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1081 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     1141 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1201 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1261 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1321 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1381 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1441 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1501 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1561 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1621 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccagatat cgacgtcctc
     1681 gaggagctca aaataacatc atcattacgt cgcactgtgg cggctatcgc actttaacgt
     1741 ttcgtgctgc cccctcagtc tatgcaatag accataaact gcaaaaaaaa gtccgctgat
     1801 aaggcttgaa aagttcattt ccagacccat ttttacatcg gtccataaac ggaattaggg
     1861 ggtaccatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
     1921 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     1981 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
     2041 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
     2101 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
     2161 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
     2221 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     2281 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     2341 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     2401 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     2461 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     2521 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     2581 taaaagctta cgcgtggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2641 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga
     2701 cctagggata tattccgctt cctcgctcac tgactcgcta cgct
//
