LOCUS       sequence-343102-        9340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows for constituitive mammalian expression of the SARS-CoV-2 3CL 
            protease via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-SARS-CoV-2-3CL
FEATURES             Location/Qualifiers
     source          1..9340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..47)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(201..789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(281..300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(960..1751,1752..1820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1583..1602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1821..1925)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1951..2406)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2088..2109)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2300..2319
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2533..2555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2547..2564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2548..2564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2574..2593
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2574..2592
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2613..2748
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2788..2909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2799..2818)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2853..2872
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2987..3220
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3292..3880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3398..3409
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3807..3827
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3931..3972)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3980..4004
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4935..4959)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4979..6157)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4988..5926
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5011..5031)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6295..6412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6455..7057)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(6541..6561)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7038..7057
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7089..7285)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(7097..7307)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7103..7238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7538..7582)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7767..8000
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8493..8618
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8665..8845
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8846..9072)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9100..9120)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9100..9118)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9139..9155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9139..9155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9152..9174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9163..9179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9187..9193,9194..9211,9212..9217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9232..9253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1921 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     1981 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     2041 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     2101 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     2161 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     2221 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     2281 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     2341 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     2401 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2461 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     2521 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     2581 actcactata gggcgaattg ggtacgtccc taggcctcca aaaaagcctc ctcactactt
     2641 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     2701 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     2761 gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa
     2821 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2881 taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca
     2941 agttataaat actgaataat aagatgacat gaactactat acgtactgct agagattttc
     3001 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
     3061 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
     3121 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa
     3181 agcagatctt gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa
     3241 aagtggctaa gatctacagc tgccttgtaa gtcattggtc ttaaagtcga cgcggggagg
     3301 cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca
     3361 gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc
     3421 agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg
     3481 atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc
     3541 agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag
     3601 tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa
     3661 acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca
     3721 gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat
     3781 acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac
     3841 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacttaa cgcgttgatt
     3901 gtcgacttaa ccggtactag ttcattacta cgtagaatcg agaccgagga gagggttagg
     3961 gataggctta ccgatatcaa ccactttgta caagaaagct gggttttatt ggaaagtaac
     4021 acctgagcat tgtctaacaa catcaaaagg tgtaaattca tcttctaata aagcactacc
     4081 caatatggta cgtccattca taccattttg cagtaattct tttaatgaag cacacatatc
     4141 taaaacggca attccagttt gagcagaaag aggtcctagt atgtcaacat ggtcttgtgt
     4201 tagaggttca taattgtact tcatagccac aaggttaaag tcattaagag ttgtggtaaa
     4261 tcgattgaga aaccacctgt ctccatttat aacagcagcg tacaaccaag ctaaaacatt
     4321 aactgtaata gttgtgtccg taccagctgc ttgtgctgtt tgcctgtcaa caaaaggtcc
     4381 ataaaagtta ccttctaagt ctgtgccagc atgaactcca gttggtaatt ccatatggtg
     4441 catgtaacaa aaagagacac agtcataatc tatgttaaaa ccaacactac cacatgaacc
     4501 attaaggaat gaacccttaa tagtgaaatt gggcctcata gcacattggt aaacaccaga
     4561 tggtgaacca ttgtaacaag ctaacactga aaaagtctgt cctggttgaa tgcgaacaaa
     4621 cttatactta ggtgtcttag gattggctgt atcaacctta agcttaagta cacaattttg
     4681 catagaatgt ccaataaccc tgagttgaac attaccagcc tgtaccaaga aattatgatt
     4741 agacttacga atgagtaaat cttcataatt agggttaagc atgtcttcag aggtgcagat
     4801 cacatgtctt ggacagtaaa ctacgtcatc aagccaaaga ccgttaagtg tagttgtacc
     4861 acaagttact tgtaccatac aaccctcaac tttaccagat gggaatgcca tttttctaaa
     4921 accactcatt ttgtagcctg cttttttgta caaacttgtt gatcaatcga tgctagcctc
     4981 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc
     5041 aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc
     5101 taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc
     5161 aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc
     5221 ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg
     5281 tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag
     5341 cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg
     5401 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
     5461 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
     5521 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
     5581 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
     5641 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
     5701 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
     5761 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
     5821 cgggatcaag aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat
     5881 aacccgtaaa gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg
     5941 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     6001 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     6061 accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg
     6121 gcgatgtgcg ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct
     6181 agaaggtcca ttagctgcaa agattcctct ctgtttaaaa ctttatccat ctttgcacct
     6241 gcaggcctcg agccggcgcc aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt
     6301 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     6361 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     6421 gaatactgcc atttgtctca agatctagaa ttcgttaact ggcaccgggc ttgcgggtca
     6481 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     6541 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     6601 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     6661 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     6721 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     6781 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     6841 cggcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     6901 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
     6961 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
     7021 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagctccggt ggtacctcta
     7081 gaaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     7141 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     7201 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     7261 atggttgctg actaattgag acgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     7321 gactttccac acctggttgc tgactaattg agatgcaatg catgggtccc ctcggggttg
     7381 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
     7441 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
     7501 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
     7561 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
     7621 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
     7681 tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga
     7741 gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag
     7801 ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt
     7861 gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc
     7921 tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga
     7981 acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct
     8041 ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat ttatataatt
     8101 cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc ggccgcttgc
     8161 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     8221 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     8281 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     8341 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     8401 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     8461 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     8521 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     8581 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     8641 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     8701 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     8761 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     8821 agatctggtc taaccagaga gacccgttta tgtatcgagc taggcactta aatacaatat
     8881 ctctgcaatg cggcaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc
     8941 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     9001 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagagtattg
     9061 cataagacta cattaagctt gcagctccag cttttgttcc ctttagtgag ggttaattgc
     9121 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9181 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9241 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9301 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
//
