LOCUS       sequence-34312-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Tan C, Marguet P, You L
  TITLE     Emergent bistability by a growth-modulating positive feedback 
            circuit.
  JOURNAL   Nat Chem Biol. 2009 Nov . 5(11):842-8.
  PUBMED    19801994
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2009 Nov . 5(11):842-8."
COMMENT     Sequence Label: pCFP
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..729
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(46..74)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     669..689
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(820..838)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      834..881
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggagaaagct accgcatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
       61 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      121 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      181 ccatggccaa cacttgtcac tactttgact tggggtgttc aatgcttttc aagataccca
      241 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      301 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      361 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      421 attcttggac acaaattgga atacaactat atttcacaca atgtatacat cactgcagac
      481 aaacaaaaga atggaatcaa agctcatttc aaaattagac acaacattga agatggaagc
      541 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      601 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      661 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      721 ctatacaaat aagagctcca ttagcatcgc tagcctagca tatgctcgag gatccggctg
      781 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      841 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctg
//
