LOCUS       sequence-343125-        2794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expression under the control of E. coli dnaK promoter engineered
            with IR3 HAIR motif from M. tuberculosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Saltepe B, Bozkurt EU, Haciosmanoglu N, Seker UOS
  TITLE     Genetic Circuits To Detect Nanomaterial Triggered Toxicity through 
            Engineered Heat Shock Response Mechanism.
  JOURNAL   ACS Synth Biol. 2019 Oct 18;8(10):2404-2417. doi: 
            10.1021/acssynbio.9b00291. Epub 2019 Oct 2.
  PUBMED    31536326
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00291"; journalName: "ACS Synth Biol"; date: 
            "2019-10-18- 18"; volume: "8"; issue: "10"; pages: "2404-2417"
COMMENT     Sequence Label: PdnaK-IR3 GFP pZa
FEATURES             Location/Qualifiers
     source          1..2794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      17..103
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(138..155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(272..817)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(352..371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(931..1025)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1046..1705)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1620..1639
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1706..1808)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     RBS             2053..2064
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2077..2790
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2107..2135)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2730..2750
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcttacgcg tggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
       61 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta
      121 gggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
      181 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
      241 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
      301 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
      361 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
      421 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
      481 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
      541 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
      601 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
      661 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
      721 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
      781 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
      841 aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
      901 catacgatat aagttgttac tagtgcttgg attctcacca ataaaaaacg cccggcggca
      961 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1021 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1081 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1141 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1201 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1261 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1321 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1381 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1441 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     1501 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1561 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1621 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1681 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1741 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1801 gccgatcaac gtctcatttt cgccagatat cgacgtcctc gagaaaagca caaaaaattt
     1861 ttgcatctcc cccttgatga cgtggtttac gaccccattt agtagtcaac cgcagtgagt
     1921 gagtctgcaa aaaaatgaaa ttgggcagtt gaaaccagac gtttcgcccc tattacagac
     1981 tcacaaccac atgatgaccg aatatatagt ggagacgttt agatggcaag cttgagcggg
     2041 gtgcactcat caaaagagga gaaaacgcgt ggtaccatgc gtaaaggaga agaacttttc
     2101 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     2161 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     2221 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     2281 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     2341 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     2401 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     2461 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     2521 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     2581 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     2641 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     2701 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     2761 attacacatg gcatggatga actatacaaa taaa
//
