LOCUS       sequence-34313-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Tan C, Marguet P, You L
  TITLE     Emergent bistability by a growth-modulating positive feedback 
            circuit.
  JOURNAL   Nat Chem Biol. 2009 Nov . 5(11):842-8.
  PUBMED    19801994
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2009 Nov . 5(11):842-8."
COMMENT     Sequence Label: pCFPT7
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..733
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(50..78)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     673..693
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     RBS             762..773
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aagaggagaa agctaccgca tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt gacttggggt gttcaatgct tttcaagata
      241 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctatatttca cacaatgtat acatcactgc
      481 agacaaacaa aagaatggaa tcaaagctca tttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataagagc tccattagca tcgctagcat taaagaggag aaagctaccg
      781 catgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg ctgctatccc
      841 gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacag
//
