LOCUS       sequence-343136-        8486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon-optimized catalytically inactive HPDL with a 
            3xFlag-V5 tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Banh RS, Kim ES, Spillier Q, Biancur DE, Yamamoto K, Sohn ASW, Shi 
            G, Jones DR, Kimmelman AC, Pacold ME
  TITLE     The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 
            synthesis pathway.
  JOURNAL   Nature. 2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w.
  PUBMED    34471290
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w."
COMMENT     Sequence Label: pLenti6.2-coHPDL H163A H258A 3xFLAG-V5
FEATURES             Location/Qualifiers
     source          1..8486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     380..399
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        461..508
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             527..925
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1012..1245
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1317..1438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1354..1373)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1408..1427
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1478..1613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1634..1652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1662..1679)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1662..1678)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1671..1693)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1820..2275
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1907..1926)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2117..2138
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2301..2405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2406..2474,2475..3266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2624..2643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3437..4025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3926..3945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4179..4196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4313..4334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4349..4354,4355..4372,4373..4379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4387..4403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4392..4414
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4411..4427
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4411..4427
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4446..4466
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4448..4466
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4494..4720
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4721..4901
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4948..5073
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5566..5799
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5984..6028
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6373..6675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6676..6879
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6830..6850
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        6924..6942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7030..7054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7058..7067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8174..8198)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8200..8265
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8284..8325
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8360..8401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        join(8434..8486,1..447)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(join(8469..8486,1..6))
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
       61 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
      121 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
      181 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
      241 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
      301 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
      361 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
      421 tcctcttcct catctccggg cctttcgact ctagacacgt gttgacaatt aatcatcggc
      481 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt
      541 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
      601 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
      661 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
      721 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
      781 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
      841 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
      901 ctctggttat gtgtgggagg gctaagcaca attcgagctc ggtaccttta agaccaatga
      961 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     1021 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     1081 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1141 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     1201 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     1261 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     1321 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1381 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1441 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     1501 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     1561 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     1621 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     1681 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     1741 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     1801 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1861 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1921 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1981 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2041 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2101 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     2161 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2221 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     2281 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2341 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2401 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2461 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2521 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2581 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2641 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2701 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2761 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2821 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2881 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2941 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3001 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3061 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3121 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3181 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3241 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3301 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4081 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4141 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4201 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4261 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4321 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4381 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4441 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     4501 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     4561 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4621 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     4681 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     4741 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4801 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4861 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4921 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     4981 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     5041 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     5101 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     5161 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     5221 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     5281 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     5341 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     5401 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     5461 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     5521 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5581 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5641 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5701 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5761 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5821 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     5881 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     5941 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     6001 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     6061 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     6121 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     6181 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     6241 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat gtcgacgtta
     6301 acgctagcga ctgggtctgc gatgtcgacg ttaacgctag cgactgggtc tgcgataagc
     6361 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     6421 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     6481 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     6541 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     6601 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct agtattagtc
     6661 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     6721 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     6781 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     6841 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     6901 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
     6961 cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg
     7021 cagatatcaa caagtttgta caaaaaagca ggcttcacca tggcggcgcc agcgctccgt
     7081 ctgtgtcaca ttgcgttcca cgttccggcc ggtcaaccac tggcgcgtaa tctgcagcgt
     7141 ctgtttggct ttcagccact ggccagccgt gaagttgatg gttggcgcca gctggcgctc
     7201 cgtagtggcg atgcggtttt tctggtgaat gagggtgccg gcagtggcga accactgtat
     7261 ggtcttgatc cacgccatgc ggtgccaagt gcgaccaatc tgtgcttcga tgtggcggat
     7321 gccggtgcgg ccacccgcga actggcggcg ctgggttgta gtgttccggt tccgccggtt
     7381 cgtgttcgcg atgcgcaagg tgccgcgacg tatgcggttg ttagcagccc ggcgggtatt
     7441 ctgagtctca cgctgctgga acgtgcgggt tatcgtggtc catttctgcc gggctttcgt
     7501 ccggttagca gtgccccggg tccgggttgg gttagtcgtg ttgacgctct gacgctggcg
     7561 tgcaccccgg gtagtagccc aacgctgctc cgttggtttc acgactgtct gggcttctgc
     7621 catctgccgc tgagtccggg cgaagatccg gaactgggtc tggaaatgac ggccggcttt
     7681 ggtctcggtg gtctgcgtct gacggccctc caagcccaac cgggcagcat tgttccaacc
     7741 ctcgttctgg cggaaagtct gccgggtgcc acgacccgtc aagatcaagt ggaacagttt
     7801 ctggcgcgcc acaaaggtcc gggtctccaa gctgtgggcc tctacacccc gaatattgtt
     7861 gaagccaccg aaggtgttgc gaccgccggt ggccaatttc tggccccacc gggcgcctac
     7921 taccaacaac cgggcaaaga gcgccaaatt cgcgcggccg gtcacgaacc acatctgctg
     7981 gcccgccaag gtattctgct cgatggcgac aagggcaagt ttctgctcca agttttcacc
     8041 aagagtctgt tcaccgaaga caccttcttt ctggagctga tccagcgtca aggcgccacg
     8101 ggttttggcc aaggcaacat ccgtgcgctg tggcagagtg ttcaagaaca gagcgcccgt
     8161 agccaagaag cccacccagc tttcttgtac aaagtggttg attacaaaga ccatgacggt
     8221 gattataaag atcatgacat cgactacaag gatgacgatg acaagggctc gggctcgacc
     8281 tcgggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacatgata actcgagtct
     8341 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     8401 gcgtaccggt tagtaatgac gatggtacct accgggtagg ggaggcgctt ttcccaaggc
     8461 agtctggagc atgcgcttta gcagcc
//
