LOCUS       sequence-343138-        8486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Banh RS, Kim ES, Spillier Q, Biancur DE, Yamamoto K, Sohn ASW, Shi 
            G, Jones DR, Kimmelman AC, Pacold ME
  TITLE     The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 
            synthesis pathway.
  JOURNAL   Nature. 2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w.
  PUBMED    34471290
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w."
COMMENT     Sequence Label: pLenti6.2-coHPDL WT 3xFlag-V5
FEATURES             Location/Qualifiers
     source          1..8486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1653..1955
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1956..2159
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2110..2130
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2204..2222
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2310..2334
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2338..2347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3454..3478)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3480..3545
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3564..3605
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3640..3681
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        3714..4213
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3749..3772)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4146..4165
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4227..4274
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4293..4691
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4778..5011
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5083..5204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5120..5139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5174..5193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5244..5379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5400..5418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5428..5445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5428..5444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5437..5459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5586..6041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5673..5692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5883..5904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6067..6171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6172..6240,6241..7032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6390..6409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7203..7791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7692..7711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7945..7962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8079..8100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8115..8120,8121..8138,8139..8145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8153..8169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8158..8180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8177..8193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8177..8193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8212..8232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8214..8232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8260..8486
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgat gtcgacgtta acgctagcga ctgggtctgc gatgtcgacg ttaacgctag
     1621 cgactgggtc tgcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     1681 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1741 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1801 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1861 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1921 cagtacatct agtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     1981 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     2041 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     2101 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     2161 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
     2221 gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg gatccactag
     2281 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggcttcacca
     2341 tggcggcgcc agcgctccgt ctgtgtcaca ttgcgttcca cgttccggcc ggtcaaccac
     2401 tggcgcgtaa tctgcagcgt ctgtttggct ttcagccact ggccagccgt gaagttgatg
     2461 gttggcgcca gctggcgctc cgtagtggcg atgcggtttt tctggtgaat gagggtgccg
     2521 gcagtggcga accactgtat ggtcttgatc cacgccatgc ggtgccaagt gcgaccaatc
     2581 tgtgcttcga tgtggcggat gccggtgcgg ccacccgcga actggcggcg ctgggttgta
     2641 gtgttccggt tccgccggtt cgtgttcgcg atgcgcaagg tgccgcgacg tatgcggttg
     2701 ttagcagccc ggcgggtatt ctgagtctca cgctgctgga acgtgcgggt tatcgtggtc
     2761 catttctgcc gggctttcgt ccggttagca gtgccccggg tccgggttgg gttagtcgtg
     2821 ttgaccatct gacgctggcg tgcaccccgg gtagtagccc aacgctgctc cgttggtttc
     2881 acgactgtct gggcttctgc catctgccgc tgagtccggg cgaagatccg gaactgggtc
     2941 tggaaatgac ggccggcttt ggtctcggtg gtctgcgtct gacggccctc caagcccaac
     3001 cgggcagcat tgttccaacc ctcgttctgg cggaaagtct gccgggtgcc acgacccgtc
     3061 aagatcaagt ggaacagttt ctggcgcgcc acaaaggtcc gggtctccaa catgtgggcc
     3121 tctacacccc gaatattgtt gaagccaccg aaggtgttgc gaccgccggt ggccaatttc
     3181 tggccccacc gggcgcctac taccaacaac cgggcaaaga gcgccaaatt cgcgcggccg
     3241 gtcacgaacc acatctgctg gcccgccaag gtattctgct cgatggcgac aagggcaagt
     3301 ttctgctcca agttttcacc aagagtctgt tcaccgaaga caccttcttt ctggagctga
     3361 tccagcgtca aggcgccacg ggttttggcc aaggcaacat ccgtgcgctg tggcagagtg
     3421 ttcaagaaca gagcgcccgt agccaagaag cccacccagc tttcttgtac aaagtggttg
     3481 attacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     3541 acaagggctc gggctcgacc tcgggtaagc ctatccctaa ccctctcctc ggtctcgatt
     3601 ctacatgata actcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     3661 ctcctcggtc tcgattctac gcgtaccggt tagtaatgac gatggtacct accgggtagg
     3721 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     3781 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     3841 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     3901 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     3961 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     4021 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     4081 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     4141 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     4201 tccgggcctt tcgactctag acacgtgttg acaattaatc atcggcatag tatatcggca
     4261 tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat
     4321 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     4381 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     4441 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     4501 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     4561 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     4621 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     4681 gggagggcta agcacaattc gagctcggta cctttaagac caatgactta caaggcagct
     4741 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4801 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4861 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4921 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4981 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     5041 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5221 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5341 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     5401 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     5461 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5521 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5581 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5641 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5701 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5761 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5821 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5881 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5941 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6001 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7321 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7801 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7861 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7921 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7981 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8041 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8101 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8161 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     8221 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     8281 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     8341 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     8401 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     8461 ttaagtgcct agctcgatac ataaac
//
