LOCUS       sequence-343139-       14873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knock out HPDL in human cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Banh RS, Kim ES, Spillier Q, Biancur DE, Yamamoto K, Sohn ASW, Shi 
            G, Jones DR, Kimmelman AC, Pacold ME
  TITLE     The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 
            synthesis pathway.
  JOURNAL   Nature. 2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w.
  PUBMED    34471290
REFERENCE   2  (bases 1 to 14873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w."
COMMENT     Sequence Label: TLCV2-sgHPDL #3
FEATURES             Location/Qualifiers
     source          1..14873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..20
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    38..56
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    73..91
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    109..127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     281..305
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      334..343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             340..4443
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             340..4443
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4444..4491
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4492..4515
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4525..4578
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4579..4581,4582..5295)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4579..4581,4582..5292)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4882..4901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5229..5250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5306..5517
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5539..6135
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5539..5558)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6035..6055
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6145..6201
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6202..6819,6820..6825,6826..6861,6862..6864,6865..6900,
                     6901..6903,6904..6939,6940..6948)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6969..7557
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7022..7042)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7440..7451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7629..7862
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7888..7905)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7894..8118
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8164..8592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8251..8270)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8461..8482
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8601..8621)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8606..8935
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8786..8921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8848..8867
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8983..9030
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9049..9423
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9553..9674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9590..9609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9644..9663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9723..9739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9723..9739)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9736..9758)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9747..9763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9771..9777,9778..9795,9796..9801))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9816..9837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9954..9971)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10125..10713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10205..10224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10884..11675,11676..11744))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11507..11526
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11745..11849)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11924..11943)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12115..12494
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12496..12694
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12712..12892
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12939..13064
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13557..13790
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13975..14019
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14317..14434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        14485..14733
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     14485..14505
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     14656..14675
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        14755..14830
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(14867..14873,1..308)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
ORIGIN
        1 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
       61 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      121 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
      241 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
      301 agatcgcctg gagaataccg gttctagagc gctgccacca tggacaagaa gtacagcatc
      361 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
      421 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
      481 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
      541 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
      601 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
      661 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
      721 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
      781 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
      841 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
      901 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
      961 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
     1021 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
     1081 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     1141 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     1201 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     1261 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     1321 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     1381 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     1441 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     1501 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
     1561 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     1621 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     1681 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     1741 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     1801 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     1861 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     1921 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     1981 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     2041 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     2101 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     2161 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     2221 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     2281 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     2341 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     2401 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     2461 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     2521 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     2581 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     2641 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     2701 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
     2761 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     2821 ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg
     2881 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
     2941 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     3001 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     3061 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     3121 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     3181 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     3241 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     3301 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     3361 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     3421 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     3481 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     3541 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     3601 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     3661 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     3721 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
     3781 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc
     3841 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     3901 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     3961 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
     4021 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     4081 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     4141 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     4201 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     4261 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     4321 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
     4381 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc
     4441 gacaagcgac ctgccgccac aaagaaggct ggacaggcta agaagaagaa agattacaaa
     4501 gacgatgacg ataagggatc cggagagggc agaggaagtc tgctaacatg cggtgacgtc
     4561 gaggagaatc ctggcccagt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4621 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4681 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4741 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4801 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4861 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4921 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4981 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5041 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5101 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5161 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     5221 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5281 gagctgtaca agtaagctag catccgggca gagcgcacat cgcccacagt ccccgagaag
     5341 ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg
     5401 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5461 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagact
     5521 gcgatcgcaa tgtacagtat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5581 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5641 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5701 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5761 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5821 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5881 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc gcccgaccac
     5941 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6001 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6061 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6121 cgcaagcccg gtgccggttc cggcgcaaca aacttctctc tgctgaaaca agccggagat
     6181 gtcgaagaga atcctggacc gatgtctaga ctggacaaga gcaaagtcat aaacggagct
     6241 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     6301 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     6361 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     6421 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     6481 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     6541 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     6601 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     6661 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     6721 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     6781 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     6841 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     6901 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact cgagcgcgtt
     6961 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7021 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7081 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7141 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7201 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7261 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7321 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7381 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7441 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7501 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     7561 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7621 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7681 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7741 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7801 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7861 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7921 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7981 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     8041 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     8101 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     8161 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8221 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8281 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8341 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8401 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8461 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8521 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8581 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8641 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8701 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8761 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8821 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8881 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8941 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     9001 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     9061 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     9121 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     9181 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     9241 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     9301 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     9361 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     9421 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     9481 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9541 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9601 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9661 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9721 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9781 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9841 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9901 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9961 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10021 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11161 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    11521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11881 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    11941 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    12001 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    12061 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
    12121 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    12181 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    12241 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    12301 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    12361 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    12421 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    12481 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
    12541 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
    12601 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
    12661 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
    12721 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    12781 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    12841 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    12901 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    12961 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    13021 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    13081 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    13141 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    13201 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    13261 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    13321 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    13381 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
    13441 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    13501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    13561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    13621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    13681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    13741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    13801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    13861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    13921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    13981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    14041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    14101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    14161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    14221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
    14281 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
    14341 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    14401 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
    14461 tccagtttgg ttaattaagg taccgagggc ctatttccca tgattccttc atatttgcat
    14521 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
    14581 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
    14641 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
    14701 gctttatata tcttgtggaa aggacgaaac accggactca gccagaacaa gagtgtttta
    14761 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
    14821 gagtcggtgc ttttttgaat tcactttggc cgcgaatcga tatgtcgagt tta
//
