LOCUS       sequence-343144-       14997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rabies vector for expressing Cre recombinase and N gene with a stop 
            codon before PEST domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Jin L, Matsuyama M, Sullivan HA, Zhu M, Lavin TK, Hou Y, Lea NE, 
            Pruner MT, Dam Ferdinez ML, Wickersham IR
  TITLE     ""Self-inactivating"" rabies viruses are susceptible to loss of 
            their intended attenuating modification.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2023481120. doi: 
            10.1073/pnas.2023481120. Epub 2023 Feb 6.
  PUBMED    37053554
REFERENCE   2  (bases 1 to 14997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-02-14"; volume: "120(7):e2023481120. 
            doi"; pages: " 10.1073/pnas.2023481120. Epub 2023 Feb 6"
COMMENT     Sequence Label: pRV∆G-N*PEST-4Cre
FEATURES             Location/Qualifiers
     source          1..14997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(301..321)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      324..333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2361..2381)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(3876..3895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3877..3895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3940..4143)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3969..3989)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4144..4523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(4565..4582)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4565..4581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4574..4596)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4722..5178)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4860..4881)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5072..5091
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5205..5309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5310..5378,5379..6170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5528..5547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6341..6929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6830..6849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7083..7100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7217..7238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7253..7258,7259..7276,7277..7283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7291..7307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7296..7318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7315..7331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7315..7331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7350..7370
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7352..7370
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7407..7423
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7407..7423
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(7446..7493)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     7489..7507
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(12506..12517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcggcggtgc agcatgttca gctggcccag gtgctgctgg atggtcttca cggccaggcc
       61 gcgggcctgc aggtacagca ggtagtcgcg cacgtcctcg ggctcggcgg ggaaccactt
      121 gcggttgttc agcttgcacc aggcggccca gctgcggcac acgctcagca gcatcttcca
      181 ggtgtgctcg ctgaaggcct ggcggtcgcg gaacatgtcc atcaggttct tgcgcacctc
      241 gtcgctggtg gcgtccacgg gcagggcggg caggttctgg tgcacggtca gcaggttgct
      301 caccttcctc ttcttcttgg gcaccatggt ggcggctttc ccggggtctt ttgagggatg
      361 ttaatagttt ttttcacatc caagaggatc aaaatgtaaa ggtaatcaac ttgactttga
      421 tgaaatgcag cggtttaatg gcaccataac atgttttttg ttatattgct cccaagggtt
      481 ggagttgagc ccatatgttc tctcctccag aggtaaacaa gtgataaatt tgcgggatat
      541 aatctgatta ttctagaagc agagaggaat ctttgtcctc ttcggacctt tgtgtctgaa
      601 gagacatgtc agaccatagt tgacatgctc tcgggttcat gttgatacac cagactctgc
      661 cctggatatg acactgtttt gcaatcactc ttatttgcaa tccgacgaac tcagtatcat
      721 catcccaagt gatctcctga gagtattcca actcctcccc ttcaagaggg cccctggaat
      781 cagcccactg gaagataaag gttctcctca atttgtatac ccagttcagg ccctcaggga
      841 ctggagatcc tgacaaagcc agtccaataa ccactttgac taacccgatc atcctatgat
      901 tcccagaata tatctcgtcg aatgatttca gaatgtgccg caggatcctg aacgagtaac
      961 cattcgggct acacacttta acccttccgt tgatacaaaa gttcctcatg ttcttcttgc
     1021 ctgtaagttc tttcagcggg acgtattcag ggggtggaag ccacaagtca tcgtcatcca
     1081 gaggggctga cgcgggagag gatttttgag tgtcctcgtc cctgcggttt ttcactatct
     1141 tacgtaggag gttcatttta tcagtggtgt tgcctgtttt tttcatgttg actttgggac
     1201 atctcggatt ttattgtcta gagggactga ggggagaggt tcggttagca agatgtatag
     1261 cgattcaagt catcttgcat gattttactc agcttgtcgg attcgactaa caactggaat
     1321 ttcttagagt tggccaaagc gacccatccc agtacacatc ttagggggag tttggacccg
     1381 tcatgggcta aacgggtcac acctggtaca ttttttgcct ctttaactat atcatcaagg
     1441 ttcattttca attgctcaaa attatacaag agtatccctg aggatcgaga gggaaactta
     1501 tattttttgg agaaactttc tgcaatctgg tgagcgatct cagcctccac tgatagatca
     1561 tcctcttcat tggtagccga ccattcaagg gctggagggc cagaagcaat ttgagccgcc
     1621 atcctggctt tcgatgattg gctttctctc tgagaaggag tgggtgttgt ctccttcttg
     1681 agctctcggc cagtagtctg ggttgattta tcctctgaag actttcctgg agggttggga
     1741 aagttgaccg cgacatagga tataatctct tctacggtct gtgaccatat cttgagaaat
     1801 ctctctcctg acctcatttg tctgactatt tggactccaa cattttccag gtatgactgg
     1861 aacaggaagc taggatcctc tccttcatcc atctgaaagt cctctcgata cttgccttct
     1921 cccaccttgg ctatctcacc atggttgggc gattttccat catccaggtg aagtcgcccc
     1981 atatcctcag ggagattgtc cacctctatg ggttcccctt ggagatgagc ctgattgtct
     2041 tcgatatttc tattgatcag atcaacagtt tcttcagcca tctcaagatc ggccagaccg
     2101 gctctaatag cactaggatt gacaaagatc ttgctcatgt ttgggatggt tcgaaaggag
     2161 gggtgttagt ttttttcatg atggatatac acaatccgta gatttccggc attttgttat
     2221 tcaacttctt acacattgat cctagcagaa gcacaggctg cagggtgacg gtccatcccg
     2281 ctctcctggg cacaagacat gggcagcgtg ccatcatcct gctcctccac ctccggcggg
     2341 aagccatggc taccggagcc actttggaag tagaggttct ctcaggaacc actgtcacta
     2401 ctatatgtct tgtttagaaa ctcggcgaat gagtttggac gggcttgatg attggaactg
     2461 actgagacat atctccgtat gtgagatctc tttagtcgac ctccattcat catgattcga
     2521 gtataaacag cctccggact tctggtttca cctgaaaagt agtcctcgtc gtcagagttg
     2581 acagttccat catctgccag tgctacgtca gtctttgtca gttcagccgc ctcgtattct
     2641 tgaagttctt tctcatctct gaagaatctt ctttcaaatg tccctttccc gaagaattcc
     2701 tctcccagat agccccctag aacagacatt tcatgaggag cacatgcagc aataaccgtt
     2761 gcatttaggg atctgacttg acccatatag catcctacaa agtgaatgag attgaacacg
     2821 tgaccaacag catttgatga ataaggagat ttcccactca agcctagtga acggaagtgg
     2881 atgaaataag agtgaggaac agctgtctcc tgccctggct caaacattct tcttatctct
     2941 tcctcaaagt tcttgtggaa gaaatatagt attgcctctc tagcggtgag attgatttgt
     3001 tttatgaacc cagtaaatga taccagtcct gaacagtctt cataagcagt gacaactgtg
     3061 cccactctga ttgctgaata tagatgctca atccgggaga aaaacatgtc ataggttccg
     3121 gccaaaaatc tgaagtttgg tatagtactc caattagcac acattttgtg agttgtcatt
     3181 agagtatggt gttccacgat tttaacaaaa ggggctgtct caaaaatctg ctctatcctg
     3241 tctgcaatgt ttgtcttata gttaccagtg ttttgcccgg atattttgct caacctatac
     3301 agactcaaga gaagaccgac taaggacgca tgctcaggga cagtggggtc tcttgtcagt
     3361 tccatgcctc ctgtcagagc ccaattccct tctacatcag tacgttttat ctccaccaga
     3421 gaacctgggg tgatcttatc tccttttcgt gcaatcacaa ttccatagct ggtccagtct
     3481 tccggacatg tcccctcaaa aaactgcatt gccgctgcca aataggaaca tacatcgtca
     3541 ggattaagtt tggcggcgct catgcctgac aaaactgact tgtatgcttt atttaaatcg
     3601 ggagcctttc ctagggttat acagggcttt ttcaaatctt tgatggcagg gtacttgtac
     3661 tcatattgat ccacgataat ctcaggcttc aaagagacca cctgattatt gactttgaat
     3721 acaatcttgt cggcatccat tgtaggggtg ttacattttt gctttgcaat tgacaatgtc
     3781 tgttttttct ttgatctggt tgttaagcgt gacccggtac gccgggtttc gtcctcacgg
     3841 actcatcaga cgcttaatct agccagcttg ggtctcccta tagtgagtcg tattaatttc
     3901 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
     3961 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     4021 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     4081 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     4141 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     4201 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     4261 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     4321 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     4381 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     4441 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     4501 ataactagtc aataatcaat gtcaacgcgc tgcaggaatt cgatatcaag cttatcgcgc
     4561 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4621 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4681 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat
     4741 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca tttttttaac caataggccg
     4801 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     4861 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     4921 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     4981 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     5041 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     5101 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     5161 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5221 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5281 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5341 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5401 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5461 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5521 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5581 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5641 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5701 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5761 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5821 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5881 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5941 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6001 ataaatctgg agcctccaag ggtgggtctc gcggtatcat tgcagcactg gggccagatg
     6061 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6121 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6181 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6241 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6481 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6961 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7021 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7081 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7141 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7201 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7261 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7321 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7381 tggagctcca ccgcggtggc ggccgctcta gaactagtgg atccggatat agttcctcct
     7441 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc
     7501 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatccccg
     7561 ccctccctta gccatccgag tggacgtgcg tcctccttcg gatgcccagg tcggaccgcg
     7621 aggaggtgga gatgccatgc cgacccacgc ttaacaaata aacaacaaaa atgagaaaaa
     7681 caatcaaaca accaaaggtt cagatttagg atcttgtttt tttcaagata catcacacaa
     7741 gagtcttagc atgggcaggc ttccaggagt atccggttca caggcaactg tagtctagta
     7801 gggatgatct agatctgata tcctctaggg tgatccacct gttgtaccta tgaaggtgtc
     7861 tttccacttc tctggataga tccttgatgc tgccaacgag tctggtagtc ttcactatct
     7921 tgtaaatcaa cctgatccag tgagatgaca gactcaggct agaattacag gctacccttt
     7981 tgaacactga ggtgtcgttg gaccaactcc aagatagata aacgttccct cgaatgactt
     8041 gctttctgaa gtaataagtt ataggtctgt tatacaggtc gtgaagtctt gcgaagctag
     8101 ggacgtcacc taaagcagta gatagataca tcatagtact gcaacatatg ttgaagtgcc
     8161 tcaggatttt gggatcagac ggtggataga acgaggggtc agttaggggt ttggaaacgt
     8221 tgaagactct gttggagaaa actatcatga tggctataat gatagccact ttagacagag
     8281 ttcccctctg taactcaaga tcatcaacca tcttgtggac tagaaaagat tctacatcac
     8341 tgtcaatcag agttatgatc atctcattgt aaggatttga tatgatttct tcaggaaatc
     8401 ctctcacaag atcctgatag ttcaaggaac gagctctctg catctcactc ttggggctgc
     8461 tacaattgaa taacacaagg ctcatttctc gaagggtgga agaggtcaag tactcagcat
     8521 ctctgaaaaa cttccctcgt ttggagaatc ggaggtagag ctcagatgaa aaagacgaag
     8581 ttacttgggt gataaaccct gtgaccgagg ggaacgctct tgacaggtgt tgaatagcct
     8641 tgtagtttgg atttactagc atagtcccat aagttttgaa gaccaaatag agtggtccat
     8701 ctatagacaa tgcaaaatcg gacattaaca gggtgatccg gttgatagat gcaatgtcag
     8761 taacttctgc atcgcaaata atgaggtcat aggacatgtt gacctgcttt tggactgact
     8821 ggaagtattt ccaggttgcc aagtttctca agtcggacgg tttttcccag attgagtcaa
     8881 gatctatcac tctggagacg atatcatttc ctcccctcat gattgctgaa ggaggcagtg
     8941 gatgtgttcc ggaagccatc aggtcattca cctctaaaag actgttgaac acaagcttgg
     9001 catctggaaa catgttgagg actgcccttg atatcccccc tgacccgtcc ccaactacaa
     9061 ggcagagaga tgggaaaaca ttgagatcat ctagaatagg cttaagctta taatgagcac
     9121 cggttgccca ctgaaccact ctcaagcccg agatcaaagg gttctggaac ctcttagaga
     9181 gggcccttat gtcaagctcc gagacagggg ccgggtttgc tgaggtagag actgcaacct
     9241 gttgagcaga gcagacccat tctgaacatc ctaccttacg ggacaccttc ttgttggggc
     9301 tgtaatctcc agtcatggtt ctagctgcat ggcgcacctc ttgatccacc catcttgtcc
     9361 ttcgtaaaga gtcttttagc agtcgttgaa tgttgtcgtc tgactctaag gtatcttctc
     9421 cgtgcccgcc cagcacctgc ctcatcaaag aactcaattg tcgcaggtta tctctcatac
     9481 tcttagatag gtttctctca accctctgga gtagaagatg agactggtaa gtaatgaggg
     9541 ataggtacgt cattttggca cttctaaagt ctgaaaagat ccatagccag tcattctctg
     9601 gagacgccgt gattatctct cgctcatagc gtagcacatg ttggagataa cacaagattg
     9661 atctgttgcc ttctttcata gtggttggat aagcggcggg gattttctga gggatagaaa
     9721 atatctctcc tctaagagac ggttctctga gcattatgta caaggaggga tggttatcta
     9781 gcctcaggag aatatatgag attacccctg agaccaattc aagaggtcta ttaatattga
     9841 tatttgtcat tcttgtcaag aagcaaatcg aggatcctat caatactccc cttgcgagcc
     9901 ctctcaaata gtctctaggg gaaaccttgc cgtatatgtt gacagggaag atggttccat
     9961 cattgtatcc tgagtcgtga attgccacta agattgagta taagagcccc tgagctgatc
    10021 cgatatggtg agacttttct ctaccgctta gagattcaaa atctcctggt ctcagacgga
    10081 tatcgggaag cctctggaag tgaggcacag ccccagaaac cattctggat attcttttcg
    10141 acacatccgg aaactcgaag atctgagagg tctccagggt cacgtcgtca atgggtctca
    10201 cacacctgtt gcatcggagg tgccaatgaa acgtagagtc tcttagcctt gtgtctctct
    10261 gtaccagctc tgatgtccat gtctgtgcat aaagcatcaa tggctggaac atgaaatcgt
    10321 agttcttccc gtcttgggtc aaatcagaca tggtgtctgt actaacagaa atatgagaga
    10381 ggaggttcgg gcagacagaa gaataccctc cttcgctgta tctggcagac ttgaacctat
    10441 gcaaggctga ccccgtcctt ttgaagacag gggcctcctc tagagggaaa tcagggcctg
    10501 tcagagacat aatgttccta attagagctt gagccaagtt ggaatctcta gtaatgaacc
    10561 agtttataga ttcttttaac gatagagctc tcttcaccac atgaacatta gtgacttttt
    10621 cccatgcatg gaatagctgg gtcgacatag aggtggacga gcccaagtat ccctttaggg
    10681 ggcctcgtga aaaaaatgac tgatcaaagg acgggagtac tgatacagaa actctaggat
    10741 tgcctcctcc tgttgctcca caagtgcaag aaatagagga cttgggaagt aatcccaaca
    10801 tctcagaagg gtgaggaact gtcgtgccta ccacttttct tccccaagag atctccctaa
    10861 gtagatctgc cctctctgaa gagcaaggcc acaccccccc aaccctctga ggtgtctggg
    10921 tcatccgact aatcccgtgg atctctgagt tgtagaagga ttcttctaaa gtttttgaga
    10981 gactctttct aaactgcctt cttatcgttc gggagttttg tatcaatcca atgattgact
    11041 cggggattcc caaaaaagac gaactgaata gctcactgag aaatcgagga aacagaggct
    11101 caacagatat taagaagagt ataaaattat ctctatgggt cttggacaac aggattgcct
    11161 ctcgaaactc tgaattttcc accttgtcca cctcgtcata taaagccttt ctgattgcat
    11221 ccttgagtag aatggtagga ctggcccctc ctctgatatt taaggtggtc ggatcttcta
    11281 gaaggcgagt gaagctctcg agtgttctct ctccaagatc tgggtttcca gcctcttgac
    11341 acaacgcgtg aatccaggac tcttgggagc ttaaccagat ctctctccag aaggataacc
    11401 cttcagagac agggtctgag aactgtcgta tatggaatct tccgagggac attccagata
    11461 tccctcccaa agaaggatct agatagatta tccttgacat ggctaggaga aagctctccc
    11521 cttcagcgct cagaatcttg taaactcttc cctttaagat tgggctaaat agcaggtagt
    11581 gaaagactgc ctgtactgac atgagcagaa aatccctcat cggtttgatc aaagattgag
    11641 agtgttgtgc cactgttagc gcattggtgg acactgtcga cattatattg gcgaggttga
    11701 ctatttggtc attagagacg caagagactc tggcccatct tttggactca ggcaccaata
    11761 tgttacctct aaacaaaggg gtttttccat agatgaggaa gtcataacta cacatggtct
    11821 cttctttctt gatgatcagc cctagcttag atgccccttc ctcgacggct ctgtatatcg
    11881 aaagtgcatt ccttgatatt ctctccaatt catagaggag cccctcttga gatagccctg
    11941 gcgacaacat gtatgtcgga cataaaacct ggttgtctcc ttgagctagt attttggttc
    12001 ttgtgttcct gatttgagat tctctatcta tcatcaataa gctgactaga ctccagccct
    12061 tctgccgtaa gccttctagc ccgccatcct ggccattcca acaggttggg ccgttggacg
    12121 catctaagca gtatatttga tcctcccgta acccgatgag gtctgatctg tctgaataat
    12181 agatccaggc cttttgaaaa aactcgtgtg ttctagaaaa cactctcttc aatccaaaca
    12241 cttgatctag gacagaaaat acatcctctg ttgactctaa tctttgatgg ttgttccact
    12301 tttcatagtc caggtgaaat gcatatgtga cccttgaata gtccaaaagc ccttgcccgg
    12361 tgaccctgtc gatcagcttt ttaaacacct tgttcaggtt gtctgtcata gtcagcgcgt
    12421 caaaaagtgg caagatgtag ttggccaaga gtttttcagt gatgacaaaa tacaatctta
    12481 gattccatga cattagagca aagaatcgac cttcaatctt caattcccgt tcctttggct
    12541 tgaggccaat tatcaagtct tcatctggca atcctccgag gtctatagac ctcagaaact
    12601 ctcggggatt gacaggcggc ttagacaggg ccgtgataat aactttttcg ctaggaacag
    12661 gcccccctcg gttttctgac agccaagaag ctagtctcgt tctggtgaaa gaatgtgatt
    12721 tgtcatccaa tatttctgac ggatccattg attcaggaat ctcaaagatc tgcgtgatcg
    12781 ggagcttgtg ccatgtatcc cccaccaagt ctacaatatg tttgggtggc catgtttggg
    12841 ttttgatata aggagtcaag gggtggtctc gggctaggaa tcttgaatcc agataccact
    12901 tggagtactt atcaaaaccc catctaagga tcctcctggc taggtcgctt gctaagcact
    12961 cctggtagga cttatctatc atttttttaa ggtgaacctg atcatatagt tttgacagac
    13021 cctttcgata atctatatat gggtgccccc aatgcctgta acagccaaac acaaaaacca
    13081 agtcatgtat gttgtcgaat tgatccagag ccctaaagaa ccttcttgca cagggaccga
    13141 acgtctcttc aagttgactt accttgtctt ttataaatac aggaaagtct cccaaggaat
    13201 gaatgagagg cctaaacttt tctgctctct ggactaaact attcacgaca tatggctcca
    13261 atattttgat gacttcatag ccggagtttc cacacataga caagacttga tccccagcaa
    13321 tgtacagctg gcatagttga gatatcaagt catctgagta tcggggctct gggggagaga
    13381 gcaagaccat taaggagttg aagcgagaca agaaaagatc ttttagcata agtgtgtagt
    13441 ttctgtcaaa aagacaattg gaactttggg agtaaacaaa gtcctttgtc acgatcagca
    13501 gtccccatat ttggtctttg aactttacca agtccttccc gatgtccact gaggttaaat
    13561 ctttccataa tgctaggatg gtcttttctt catcccagtc tagggcgttc atgtataagg
    13621 tgattacatg gaagaacaag taagaggaat acgtgttggc aagatacctt ccaaatgcat
    13681 tatcataatc aaccctctca aggcaactta acactccctc tggggggatt ctcagccctc
    13741 tatttcctag cgtgagattc aacagcttct ctatggggga cgacttggaa tagaaatggg
    13801 ccaagtctgt tatacatctc cggctcctgt tggattcaga gtgggcacca tataaccaga
    13861 gagaaatcat tgactgtgca gccattccgc ccaccttgag agaacccaaa tctaccttct
    13921 tgaaataatc tttcaaaact ctgaaagacc tggagcaatt gtctgttaga gtcatccgat
    13981 aaggtctatt ccctgttttt aaccattcta acattagtct agcaggatct tctatcaaag
    14041 gagagttgag attgtagtca gagttcctca agatgttggg gacaatgggg gttcctctgg
    14101 gttcagcctc taactcgatt gggtcaatag ggtcatcata gacctctcca ggatcgagca
    14161 tcttgaagta agtctcaggt tgagaagtgt tgccagttgt tcttctgatc taatgttttt
    14221 ttctcgactg aaaagcttag atgacccagc actttataag ctagctcatc agtcgccgtc
    14281 ctccagcagg cgcaccatgg cgccggtctc gctgtccagg ttgcggatgt agttcatcac
    14341 gatgttcacg ttggtccagc cgccggcctg catgatctcg gggatgctca cgccggcgcg
    14401 ggccatgtcg cgggcggcgc ccacgcgggc gctgtggccg ctccaggcca ggtagcgctg
    14461 gccgctgtcg tccttggcgc cgtagatcag gcggtgggtg gcctcgaaga tgccctccag
    14521 ggcccgggtg ctcagctggc tggtggcgct gggggcggcc acgccgttct tgcgcacgcg
    14581 gcagaacagg tagttgttgg ggtcgtcggc cacgccgctc acgctgatcc agcgctccac
    14641 cagcttggtc acgcccaggc tcagggcctt ctccacgccg gcggtgctca ccagggtctt
    14701 ggtgcggccg atgtggatca gcatgcggcc gccgtcggtg cggctgatgt ccttcacgcg
    14761 gatgcgggcg atctcggcga tgcgcagcag ggtgttgtag gcgatgccca ggaaggccag
    14821 gttgcggatg tcctggcagc ggtcgctgtt ctccatcagg ctgcgcacct ggtcgaagtc
    14881 ggtgcgctcg aaggccaggg cctgcttggc gcgctcgccg gcgtccacgt tctccttgcg
    14941 gatgcggcgc atcaccaggc tcacggcgtt gctgtcgctg gggcggggca ggccgct
//
