LOCUS       sequence-343145-       14997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rabies vector for expressing Cre recombinase and N gene with PEST 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Jin L, Matsuyama M, Sullivan HA, Zhu M, Lavin TK, Hou Y, Lea NE, 
            Pruner MT, Dam Ferdinez ML, Wickersham IR
  TITLE     ""Self-inactivating"" rabies viruses are susceptible to loss of 
            their intended attenuating modification.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2023481120. doi: 
            10.1073/pnas.2023481120. Epub 2023 Feb 6.
  PUBMED    37053554
REFERENCE   2  (bases 1 to 14997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-02-14"; volume: "120(7):e2023481120. 
            doi"; pages: " 10.1073/pnas.2023481120. Epub 2023 Feb 6"
COMMENT     Sequence Label: pRV∆G-NPEST-4Cre
FEATURES             Location/Qualifiers
     source          1..14997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1763..1783
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      3811..3820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3823..3843
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6624..6635
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11634..11652)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11648..11695
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11718..11734)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11718..11734)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11771..11791)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11771..11789)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11810..11826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11810..11826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11823..11845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11834..11850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11858..11864,11865..11882,11883..11888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11903..11924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12041..12058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12212..12800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12292..12311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12971..13762,13763..13831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13594..13613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13832..13936)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13963..14419
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14050..14069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14260..14281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14545..14567
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14559..14576
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14560..14576
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14618..14997
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagattaa gcgtctgatg
      301 agtccgtgag gacgaaaccc ggcgtaccgg gtcacgctta acaaccagat caaagaaaaa
      361 acagacattg tcaattgcaa agcaaaaatg taacacccct acaatggatg ccgacaagat
      421 tgtattcaaa gtcaataatc aggtggtctc tttgaagcct gagattatcg tggatcaata
      481 tgagtacaag taccctgcca tcaaagattt gaaaaagccc tgtataaccc taggaaaggc
      541 tcccgattta aataaagcat acaagtcagt tttgtcaggc atgagcgccg ccaaacttaa
      601 tcctgacgat gtatgttcct atttggcagc ggcaatgcag ttttttgagg ggacatgtcc
      661 ggaagactgg accagctatg gaattgtgat tgcacgaaaa ggagataaga tcaccccagg
      721 ttctctggtg gagataaaac gtactgatgt agaagggaat tgggctctga caggaggcat
      781 ggaactgaca agagacccca ctgtccctga gcatgcgtcc ttagtcggtc ttctcttgag
      841 tctgtatagg ttgagcaaaa tatccgggca aaacactggt aactataaga caaacattgc
      901 agacaggata gagcagattt ttgagacagc cccttttgtt aaaatcgtgg aacaccatac
      961 tctaatgaca actcacaaaa tgtgtgctaa ttggagtact ataccaaact tcagattttt
     1021 ggccggaacc tatgacatgt ttttctcccg gattgagcat ctatattcag caatcagagt
     1081 gggcacagtt gtcactgctt atgaagactg ttcaggactg gtatcattta ctgggttcat
     1141 aaaacaaatc aatctcaccg ctagagaggc aatactatat ttcttccaca agaactttga
     1201 ggaagagata agaagaatgt ttgagccagg gcaggagaca gctgttcctc actcttattt
     1261 catccacttc cgttcactag gcttgagtgg gaaatctcct tattcatcaa atgctgttgg
     1321 tcacgtgttc aatctcattc actttgtagg atgctatatg ggtcaagtca gatccctaaa
     1381 tgcaacggtt attgctgcat gtgctcctca tgaaatgtct gttctagggg gctatctggg
     1441 agaggaattc ttcgggaaag ggacatttga aagaagattc ttcagagatg agaaagaact
     1501 tcaagaatac gaggcggctg aactgacaaa gactgacgta gcactggcag atgatggaac
     1561 tgtcaactct gacgacgagg actacttttc aggtgaaacc agaagtccgg aggctgttta
     1621 tactcgaatc atgatgaatg gaggtcgact aaagagatct cacatacgga gatatgtctc
     1681 agtcagttcc aatcatcaag cccgtccaaa ctcattcgcc gagtttctaa acaagacata
     1741 tagtagtgac agtggttccg gcgagaacct ctacttccaa agtggctccg gtagccatgg
     1801 cttcccgccg gaggtggagg agcaggatga tggcacgctg cccatgtctt gtgcccagga
     1861 gagcgggatg gaccgtcacc ctgcagcctg tgcttctgct aggatcaatg tgtaagaagt
     1921 tgaataacaa aatgccggaa atctacggat tgtgtatatc catcatgaaa aaaactaaca
     1981 cccctccttt cgaaccatcc caaacatgag caagatcttt gtcaatccta gtgctattag
     2041 agccggtctg gccgatcttg agatggctga agaaactgtt gatctgatca atagaaatat
     2101 cgaagacaat caggctcatc tccaagggga acccatagag gtggacaatc tccctgagga
     2161 tatggggcga cttcacctgg atgatggaaa atcgcccaac catggtgaga tagccaaggt
     2221 gggagaaggc aagtatcgag aggactttca gatggatgaa ggagaggatc ctagcttcct
     2281 gttccagtca tacctggaaa atgttggagt ccaaatagtc agacaaatga ggtcaggaga
     2341 gagatttctc aagatatggt cacagaccgt agaagagatt atatcctatg tcgcggtcaa
     2401 ctttcccaac cctccaggaa agtcttcaga ggataaatca acccagacta ctggccgaga
     2461 gctcaagaag gagacaacac ccactccttc tcagagagaa agccaatcat cgaaagccag
     2521 gatggcggct caaattgctt ctggccctcc agcccttgaa tggtcggcta ccaatgaaga
     2581 ggatgatcta tcagtggagg ctgagatcgc tcaccagatt gcagaaagtt tctccaaaaa
     2641 atataagttt ccctctcgat cctcagggat actcttgtat aattttgagc aattgaaaat
     2701 gaaccttgat gatatagtta aagaggcaaa aaatgtacca ggtgtgaccc gtttagccca
     2761 tgacgggtcc aaactccccc taagatgtgt actgggatgg gtcgctttgg ccaactctaa
     2821 gaaattccag ttgttagtcg aatccgacaa gctgagtaaa atcatgcaag atgacttgaa
     2881 tcgctataca tcttgctaac cgaacctctc ccctcagtcc ctctagacaa taaaatccga
     2941 gatgtcccaa agtcaacatg aaaaaaacag gcaacaccac tgataaaatg aacctcctac
     3001 gtaagatagt gaaaaaccgc agggacgagg acactcaaaa atcctctccc gcgtcagccc
     3061 ctctggatga cgatgacttg tggcttccac cccctgaata cgtcccgctg aaagaactta
     3121 caggcaagaa gaacatgagg aacttttgta tcaacggaag ggttaaagtg tgtagcccga
     3181 atggttactc gttcaggatc ctgcggcaca ttctgaaatc attcgacgag atatattctg
     3241 ggaatcatag gatgatcggg ttagtcaaag tggttattgg actggctttg tcaggatctc
     3301 cagtccctga gggcctgaac tgggtataca aattgaggag aacctttatc ttccagtggg
     3361 ctgattccag gggccctctt gaaggggagg agttggaata ctctcaggag atcacttggg
     3421 atgatgatac tgagttcgtc ggattgcaaa taagagtgat tgcaaaacag tgtcatatcc
     3481 agggcagagt ctggtgtatc aacatgaacc cgagagcatg tcaactatgg tctgacatgt
     3541 ctcttcagac acaaaggtcc gaagaggaca aagattcctc tctgcttcta gaataatcag
     3601 attatatccc gcaaatttat cacttgttta cctctggagg agagaacata tgggctcaac
     3661 tccaaccctt gggagcaata taacaaaaaa catgttatgg tgccattaaa ccgctgcatt
     3721 tcatcaaagt caagttgatt acctttacat tttgatcctc ttggatgtga aaaaaactat
     3781 taacatccct caaaagaccc cgggaaagcc gccaccatgg tgcccaagaa gaagaggaag
     3841 gtgagcaacc tgctgaccgt gcaccagaac ctgcccgccc tgcccgtgga cgccaccagc
     3901 gacgaggtgc gcaagaacct gatggacatg ttccgcgacc gccaggcctt cagcgagcac
     3961 acctggaaga tgctgctgag cgtgtgccgc agctgggccg cctggtgcaa gctgaacaac
     4021 cgcaagtggt tccccgccga gcccgaggac gtgcgcgact acctgctgta cctgcaggcc
     4081 cgcggcctgg ccgtgaagac catccagcag cacctgggcc agctgaacat gctgcaccgc
     4141 cgcagcggcc tgccccgccc cagcgacagc aacgccgtga gcctggtgat gcgccgcatc
     4201 cgcaaggaga acgtggacgc cggcgagcgc gccaagcagg ccctggcctt cgagcgcacc
     4261 gacttcgacc aggtgcgcag cctgatggag aacagcgacc gctgccagga catccgcaac
     4321 ctggccttcc tgggcatcgc ctacaacacc ctgctgcgca tcgccgagat cgcccgcatc
     4381 cgcgtgaagg acatcagccg caccgacggc ggccgcatgc tgatccacat cggccgcacc
     4441 aagaccctgg tgagcaccgc cggcgtggag aaggccctga gcctgggcgt gaccaagctg
     4501 gtggagcgct ggatcagcgt gagcggcgtg gccgacgacc ccaacaacta cctgttctgc
     4561 cgcgtgcgca agaacggcgt ggccgccccc agcgccacca gccagctgag cacccgggcc
     4621 ctggagggca tcttcgaggc cacccaccgc ctgatctacg gcgccaagga cgacagcggc
     4681 cagcgctacc tggcctggag cggccacagc gcccgcgtgg gcgccgcccg cgacatggcc
     4741 cgcgccggcg tgagcatccc cgagatcatg caggccggcg gctggaccaa cgtgaacatc
     4801 gtgatgaact acatccgcaa cctggacagc gagaccggcg ccatggtgcg cctgctggag
     4861 gacggcgact gatgagctag cttataaagt gctgggtcat ctaagctttt cagtcgagaa
     4921 aaaaacatta gatcagaaga acaactggca acacttctca acctgagact tacttcaaga
     4981 tgctcgatcc tggagaggtc tatgatgacc ctattgaccc aatcgagtta gaggctgaac
     5041 ccagaggaac ccccattgtc cccaacatct tgaggaactc tgactacaat ctcaactctc
     5101 ctttgataga agatcctgct agactaatgt tagaatggtt aaaaacaggg aatagacctt
     5161 atcggatgac tctaacagac aattgctcca ggtctttcag agttttgaaa gattatttca
     5221 agaaggtaga tttgggttct ctcaaggtgg gcggaatggc tgcacagtca atgatttctc
     5281 tctggttata tggtgcccac tctgaatcca acaggagccg gagatgtata acagacttgg
     5341 cccatttcta ttccaagtcg tcccccatag agaagctgtt gaatctcacg ctaggaaata
     5401 gagggctgag aatcccccca gagggagtgt taagttgcct tgagagggtt gattatgata
     5461 atgcatttgg aaggtatctt gccaacacgt attcctctta cttgttcttc catgtaatca
     5521 ccttatacat gaacgcccta gactgggatg aagaaaagac catcctagca ttatggaaag
     5581 atttaacctc agtggacatc gggaaggact tggtaaagtt caaagaccaa atatggggac
     5641 tgctgatcgt gacaaaggac tttgtttact cccaaagttc caattgtctt tttgacagaa
     5701 actacacact tatgctaaaa gatcttttct tgtctcgctt caactcctta atggtcttgc
     5761 tctctccccc agagccccga tactcagatg acttgatatc tcaactatgc cagctgtaca
     5821 ttgctgggga tcaagtcttg tctatgtgtg gaaactccgg ctatgaagtc atcaaaatat
     5881 tggagccata tgtcgtgaat agtttagtcc agagagcaga aaagtttagg cctctcattc
     5941 attccttggg agactttcct gtatttataa aagacaaggt aagtcaactt gaagagacgt
     6001 tcggtccctg tgcaagaagg ttctttaggg ctctggatca attcgacaac atacatgact
     6061 tggtttttgt gtttggctgt tacaggcatt gggggcaccc atatatagat tatcgaaagg
     6121 gtctgtcaaa actatatgat caggttcacc ttaaaaaaat gatagataag tcctaccagg
     6181 agtgcttagc aagcgaccta gccaggagga tccttagatg gggttttgat aagtactcca
     6241 agtggtatct ggattcaaga ttcctagccc gagaccaccc cttgactcct tatatcaaaa
     6301 cccaaacatg gccacccaaa catattgtag acttggtggg ggatacatgg cacaagctcc
     6361 cgatcacgca gatctttgag attcctgaat caatggatcc gtcagaaata ttggatgaca
     6421 aatcacattc tttcaccaga acgagactag cttcttggct gtcagaaaac cgaggggggc
     6481 ctgttcctag cgaaaaagtt attatcacgg ccctgtctaa gccgcctgtc aatccccgag
     6541 agtttctgag gtctatagac ctcggaggat tgccagatga agacttgata attggcctca
     6601 agccaaagga acgggaattg aagattgaag gtcgattctt tgctctaatg tcatggaatc
     6661 taagattgta ttttgtcatc actgaaaaac tcttggccaa ctacatcttg ccactttttg
     6721 acgcgctgac tatgacagac aacctgaaca aggtgtttaa aaagctgatc gacagggtca
     6781 ccgggcaagg gcttttggac tattcaaggg tcacatatgc atttcacctg gactatgaaa
     6841 agtggaacaa ccatcaaaga ttagagtcaa cagaggatgt attttctgtc ctagatcaag
     6901 tgtttggatt gaagagagtg ttttctagaa cacacgagtt ttttcaaaag gcctggatct
     6961 attattcaga cagatcagac ctcatcgggt tacgggagga tcaaatatac tgcttagatg
     7021 cgtccaacgg cccaacctgt tggaatggcc aggatggcgg gctagaaggc ttacggcaga
     7081 agggctggag tctagtcagc ttattgatga tagatagaga atctcaaatc aggaacacaa
     7141 gaaccaaaat actagctcaa ggagacaacc aggttttatg tccgacatac atgttgtcgc
     7201 cagggctatc tcaagagggg ctcctctatg aattggagag aatatcaagg aatgcacttt
     7261 cgatatacag agccgtcgag gaaggggcat ctaagctagg gctgatcatc aagaaagaag
     7321 agaccatgtg tagttatgac ttcctcatct atggaaaaac ccctttgttt agaggtaaca
     7381 tattggtgcc tgagtccaaa agatgggcca gagtctcttg cgtctctaat gaccaaatag
     7441 tcaacctcgc caatataatg tcgacagtgt ccaccaatgc gctaacagtg gcacaacact
     7501 ctcaatcttt gatcaaaccg atgagggatt ttctgctcat gtcagtacag gcagtctttc
     7561 actacctgct atttagccca atcttaaagg gaagagttta caagattctg agcgctgaag
     7621 gggagagctt tctcctagcc atgtcaagga taatctatct agatccttct ttgggaggga
     7681 tatctggaat gtccctcgga agattccata tacgacagtt ctcagaccct gtctctgaag
     7741 ggttatcctt ctggagagag atctggttaa gctcccaaga gtcctggatt cacgcgttgt
     7801 gtcaagaggc tggaaaccca gatcttggag agagaacact cgagagcttc actcgccttc
     7861 tagaagatcc gaccacctta aatatcagag gaggggccag tcctaccatt ctactcaagg
     7921 atgcaatcag aaaggcttta tatgacgagg tggacaaggt ggaaaattca gagtttcgag
     7981 aggcaatcct gttgtccaag acccatagag ataattttat actcttctta atatctgttg
     8041 agcctctgtt tcctcgattt ctcagtgagc tattcagttc gtcttttttg ggaatccccg
     8101 agtcaatcat tggattgata caaaactccc gaacgataag aaggcagttt agaaagagtc
     8161 tctcaaaaac tttagaagaa tccttctaca actcagagat ccacgggatt agtcggatga
     8221 cccagacacc tcagagggtt gggggggtgt ggccttgctc ttcagagagg gcagatctac
     8281 ttagggagat ctcttgggga agaaaagtgg taggcacgac agttcctcac ccttctgaga
     8341 tgttgggatt acttcccaag tcctctattt cttgcacttg tggagcaaca ggaggaggca
     8401 atcctagagt ttctgtatca gtactcccgt cctttgatca gtcatttttt tcacgaggcc
     8461 ccctaaaggg atacttgggc tcgtccacct ctatgtcgac ccagctattc catgcatggg
     8521 aaaaagtcac taatgttcat gtggtgaaga gagctctatc gttaaaagaa tctataaact
     8581 ggttcattac tagagattcc aacttggctc aagctctaat taggaacatt atgtctctga
     8641 caggccctga tttccctcta gaggaggccc ctgtcttcaa aaggacgggg tcagccttgc
     8701 ataggttcaa gtctgccaga tacagcgaag gagggtattc ttctgtctgc ccgaacctcc
     8761 tctctcatat ttctgttagt acagacacca tgtctgattt gacccaagac gggaagaact
     8821 acgatttcat gttccagcca ttgatgcttt atgcacagac atggacatca gagctggtac
     8881 agagagacac aaggctaaga gactctacgt ttcattggca cctccgatgc aacaggtgtg
     8941 tgagacccat tgacgacgtg accctggaga cctctcagat cttcgagttt ccggatgtgt
     9001 cgaaaagaat atccagaatg gtttctgggg ctgtgcctca cttccagagg cttcccgata
     9061 tccgtctgag accaggagat tttgaatctc taagcggtag agaaaagtct caccatatcg
     9121 gatcagctca ggggctctta tactcaatct tagtggcaat tcacgactca ggatacaatg
     9181 atggaaccat cttccctgtc aacatatacg gcaaggtttc ccctagagac tatttgagag
     9241 ggctcgcaag gggagtattg ataggatcct cgatttgctt cttgacaaga atgacaaata
     9301 tcaatattaa tagacctctt gaattggtct caggggtaat ctcatatatt ctcctgaggc
     9361 tagataacca tccctccttg tacataatgc tcagagaacc gtctcttaga ggagagatat
     9421 tttctatccc tcagaaaatc cccgccgctt atccaaccac tatgaaagaa ggcaacagat
     9481 caatcttgtg ttatctccaa catgtgctac gctatgagcg agagataatc acggcgtctc
     9541 cagagaatga ctggctatgg atcttttcag actttagaag tgccaaaatg acgtacctat
     9601 ccctcattac ttaccagtct catcttctac tccagagggt tgagagaaac ctatctaaga
     9661 gtatgagaga taacctgcga caattgagtt ctttgatgag gcaggtgctg ggcgggcacg
     9721 gagaagatac cttagagtca gacgacaaca ttcaacgact gctaaaagac tctttacgaa
     9781 ggacaagatg ggtggatcaa gaggtgcgcc atgcagctag aaccatgact ggagattaca
     9841 gccccaacaa gaaggtgtcc cgtaaggtag gatgttcaga atgggtctgc tctgctcaac
     9901 aggttgcagt ctctacctca gcaaacccgg cccctgtctc ggagcttgac ataagggccc
     9961 tctctaagag gttccagaac cctttgatct cgggcttgag agtggttcag tgggcaaccg
    10021 gtgctcatta taagcttaag cctattctag atgatctcaa tgttttccca tctctctgcc
    10081 ttgtagttgg ggacgggtca ggggggatat caagggcagt cctcaacatg tttccagatg
    10141 ccaagcttgt gttcaacagt cttttagagg tgaatgacct gatggcttcc ggaacacatc
    10201 cactgcctcc ttcagcaatc atgaggggag gaaatgatat cgtctccaga gtgatagatc
    10261 ttgactcaat ctgggaaaaa ccgtccgact tgagaaactt ggcaacctgg aaatacttcc
    10321 agtcagtcca aaagcaggtc aacatgtcct atgacctcat tatttgcgat gcagaagtta
    10381 ctgacattgc atctatcaac cggatcaccc tgttaatgtc cgattttgca ttgtctatag
    10441 atggaccact ctatttggtc ttcaaaactt atgggactat gctagtaaat ccaaactaca
    10501 aggctattca acacctgtca agagcgttcc cctcggtcac agggtttatc acccaagtaa
    10561 cttcgtcttt ttcatctgag ctctacctcc gattctccaa acgagggaag tttttcagag
    10621 atgctgagta cttgacctct tccacccttc gagaaatgag ccttgtgtta ttcaattgta
    10681 gcagccccaa gagtgagatg cagagagctc gttccttgaa ctatcaggat cttgtgagag
    10741 gatttcctga agaaatcata tcaaatcctt acaatgagat gatcataact ctgattgaca
    10801 gtgatgtaga atcttttcta gtccacaaga tggttgatga tcttgagtta cagaggggaa
    10861 ctctgtctaa agtggctatc attatagcca tcatgatagt tttctccaac agagtcttca
    10921 acgtttccaa acccctaact gacccctcgt tctatccacc gtctgatccc aaaatcctga
    10981 ggcacttcaa catatgttgc agtactatga tgtatctatc tactgcttta ggtgacgtcc
    11041 ctagcttcgc aagacttcac gacctgtata acagacctat aacttattac ttcagaaagc
    11101 aagtcattcg agggaacgtt tatctatctt ggagttggtc caacgacacc tcagtgttca
    11161 aaagggtagc ctgtaattct agcctgagtc tgtcatctca ctggatcagg ttgatttaca
    11221 agatagtgaa gactaccaga ctcgttggca gcatcaagga tctatccaga gaagtggaaa
    11281 gacaccttca taggtacaac aggtggatca ccctagagga tatcagatct agatcatccc
    11341 tactagacta cagttgcctg tgaaccggat actcctggaa gcctgcccat gctaagactc
    11401 ttgtgtgatg tatcttgaaa aaaacaagat cctaaatctg aacctttggt tgtttgattg
    11461 tttttctcat ttttgttgtt tatttgttaa gcgtgggtcg gcatggcatc tccacctcct
    11521 cgcggtccga cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggaggg
    11581 cggggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
    11641 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
    11701 aggaggaact atatccggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
    11761 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
    11821 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    11881 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    11941 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    12001 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    12061 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    12121 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    12181 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    12241 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    12301 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    12361 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    12421 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    12481 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    12541 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    12601 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    12661 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    12721 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    12781 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    12841 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    12901 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    12961 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    13021 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    13081 catctggccc cagtgctgca atgataccgc gagacccacc cttggaggct ccagatttat
    13141 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    13201 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    13261 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    13321 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    13381 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    13441 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    13501 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    13561 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    13621 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    13681 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    13741 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    13801 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    13861 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    13921 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
    13981 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    14041 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    14101 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    14161 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    14221 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    14281 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    14341 cggcctattg gttaaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    14401 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    14461 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    14521 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    14581 gcgcgataag cttgatatcg aattcctgca gcgcgttgac attgattatt gactagttat
    14641 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    14701 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    14761 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    14821 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    14881 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    14941 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatg
//
