LOCUS       sequence-343155-        1300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of APEX2 in the periplasm from a codon-optimized gene 
            (multi-copy episomal plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ahamed M, Jaisinghani N, Li M, Winkeler I, Silva S, Previti ML, 
            Seeliger JC
  TITLE     Optimized APEX2 peroxidase-mediated proximity labeling in fast- and 
            slow-growing mycobacteria.
  JOURNAL   Methods Enzymol. 2022;664:267-289. doi: 10.1016/bs.mie.2021.11.021. 
            Epub 2021 Dec 30.
  PUBMED    35331378
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2022"; volume: "664:267-289. doi"; pages: " 
            10.1016/bs.mie.2021.11.021. Epub 2021 Dec 30"
COMMENT     Sequence Label: pRibo-Sec-APEX2m
FEATURES             Location/Qualifiers
     source          1..1300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      339..348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1212..1235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctagaggtg accacaacgc gcccgctttg atcggggacg tctgcggccg accatttacg
       61 ggtcttgttg tcgttggcgg tcatgggccg aacatactca cccggatcgg agggccgagg
      121 acaaggtcga acgaggggca tgacccggtg cggggcttct tgcactcggc ataggcgagt
      181 gctaagaata acgttggcac tcgcgaccgg gatacgactc actataggta ccggtgatac
      241 cagcatcgtc ttgatgccct tggcagcacc ctgctaagga ggcaacaaga tgcatatgaa
      301 gctcaccaca atgatcaaga cggcagtagc ggtcgtggcc atggcggcca tcgcgacctt
      361 tgcggcaccg gtcgcgttgg ctggatccgg caagagctac ccgaccgtca gcgccgacta
      421 ccaggacgcc gtggagaagg ccaagaagaa gctgcgcggc ttcatcgccg agaagcggtg
      481 cgccccgctg atgctgcgcc tggcgttcca cagcgcgggc accttcgaca agggcaccaa
      541 gaccggcggc ccgttcggca ccatcaagca cccggcggag ctggcgcaca gcgccaacaa
      601 cggcctggac atcgcggtcc ggctgctgga gccgctgaag gcggagttcc cgatcctgag
      661 ctacgccgac ttctaccagc tggcgggcgt cgtggccgtc gaggtgaccg gcggcccgaa
      721 ggtgccgttc cacccgggcc gggaggacaa gccggagccg ccgccggagg gccgcctgcc
      781 ggacccgacc aagggctcgg accacctgcg ggacgtcttc ggcaaggcga tgggcctgac
      841 cgaccaggac atcgtggccc tgagcggcgg ccacaccatc ggcgcggccc acaaggagcg
      901 cagcggcttc gagggcccgt ggaccagcaa cccgctgatc ttcgacaact cgtacttcac
      961 cgagctgctg agcggcgaga aggaaggcct gctgcagctg ccgagcgaca aggcgctgct
     1021 gagcgacccg gtcttccggc ccctggtgga caagtacgcg gccgacgagg acgccttctt
     1081 cgccgactac gccgaggccc accagaagct gtccgagctg ggcttcgccg acgccaagct
     1141 ttgaatcgat acatcaggaa ttcgctagcg actacaagga cgacgacggc gattataaag
     1201 atcatgacat cgactacaag gacgacgacg acaagtaggt agttaactag cgtacgatcg
     1261 actgccaggc atcaaataaa acgaaaggct cagtcgaaag
//
