LOCUS       sequence-343159-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of APEX2 from a codon-optimized gene (single-copy 
            integrating plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ahamed M, Jaisinghani N, Li M, Winkeler I, Silva S, Previti ML, 
            Seeliger JC
  TITLE     Optimized APEX2 peroxidase-mediated proximity labeling in fast- and 
            slow-growing mycobacteria.
  JOURNAL   Methods Enzymol. 2022;664:267-289. doi: 10.1016/bs.mie.2021.11.021. 
            Epub 2021 Dec 30.
  PUBMED    35331378
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2022"; volume: "664:267-289. doi"; pages: " 
            10.1016/bs.mie.2021.11.021. Epub 2021 Dec 30"
COMMENT     Sequence Label: pRiboI-APEX2m
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      1098..1144
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagaggtg accacaacgc gcccgctttg atcggggacg tctgcggccg accatttacg
       61 ggtcttgttg tcgttggcgg tcatgggccg aacatactca cccggatcgg agggccgagg
      121 acaaggtcga acgaggggca tgacccggtg cggggcttct tgcactcggc ataggcgagt
      181 gctaagaata acgttggcac tcgcgaccgg gatacgactc actataggta ccggtgatac
      241 cagcatcgtc ttgatgccct tggcagcacc ctgctaagga ggcaacaaga tgcatatggg
      301 caagagctac ccgaccgtca gcgccgacta ccaggacgcc gtggagaagg ccaagaagaa
      361 gctgcgcggc ttcatcgccg agaagcggtg cgccccgctg atgctgcgcc tggcgttcca
      421 cagcgcgggc accttcgaca agggcaccaa gaccggcggc ccgttcggca ccatcaagca
      481 cccggcggag ctggcgcaca gcgccaacaa cggcctggac atcgcggtcc ggctgctgga
      541 gccgctgaag gcggagttcc cgatcctgag ctacgccgac ttctaccagc tggcgggcgt
      601 cgtggccgtc gaggtgaccg gcggcccgaa ggtgccgttc cacccgggcc gggaggacaa
      661 gccggagccg ccgccggagg gccgcctgcc ggacccgacc aagggctcgg accacctgcg
      721 ggacgtcttc ggcaaggcga tgggcctgac cgaccaggac atcgtggccc tgagcggcgg
      781 ccacaccatc ggcgcggccc acaaggagcg cagcggcttc gagggcccgt ggaccagcaa
      841 cccgctgatc ttcgacaact cgtacttcac cgagctgctg agcggcgaga aggaaggcct
      901 gctgcagctg ccgagcgaca aggcgctgct gagcgacccg gtcttccggc ccctggtgga
      961 caagtacgcg gccgacgagg acgccttctt cgccgactac gccgaggccc accagaagct
     1021 gtccgagctg ggcttcgccg acgccaagct tatcgatgtc gacgtagtta actagcgtac
     1081 gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1141 ttat
//
