LOCUS       sequence-343162-        6319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAPRIN1 knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pAPM-miR30-CAPRIN1-ts3
FEATURES             Location/Qualifiers
     source          1..6319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..37)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(211..231)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        216..545
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      396..531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     458..477
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        557..936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        938..1136
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1091..1111
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1154..1334
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1381..1506
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2003..2236
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2421..2465
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2763..2880
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3017..3424
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     ncRNA           4139..4153
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     LTR             4287..4467
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4538..5126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4618..4637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5297..6088,6089..6157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5920..5939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6158..6262)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
       61 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
      121 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
      181 cgcgatgtac gggccagata tacgcgctgt ggaatgtgtg tcagttaggg tgtggaaagt
      241 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      301 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      361 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      421 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      481 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      541 gcaaaaagct cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      601 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      661 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      721 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      781 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      841 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      901 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      961 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1021 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1081 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     1141 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     1201 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     1261 tgcccgtctg ttgtgagact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1321 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     1381 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     1441 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1501 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1561 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1621 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1681 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1741 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1801 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     1861 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1921 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1981 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     2041 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     2101 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     2161 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     2221 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     2281 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgactt
     2341 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2401 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2461 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2521 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2581 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2641 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2701 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     2761 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2821 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2881 cgggtttatt acagggacag cagagatcca gtttggttaa ttaactgcag ccccgataaa
     2941 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     3001 tggcaagcta gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat
     3061 agagaagttc agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata
     3121 tctgcggtga gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc
     3181 ggccccggcc cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt
     3241 cttaagaccc atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta
     3301 tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct
     3361 ctataaaaga gctcacaacc cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc
     3421 cgggggtcta gaacgcgtgc cgccatgacc gaatacaaac ctaccgtgag gctggctaca
     3481 agagatgatg tcccaagggc tgtgagaaca ctggccgccg cttttgccga ttaccctgcc
     3541 acacgccaca ctgtggaccc agatcggcat atcgagagag tgactgagct gcaggaactg
     3601 ttcctgaccc gagtgggcct ggacattggg aaggtctggg tcgcagacga tggagcagct
     3661 gtggctgtct ggaccacacc agagagcgtg gaagccggag ctgtctttgc agagatcggc
     3721 cctagaatgg cagaactgag cggctccagg ctggcagcac agcagcagat ggagggactg
     3781 ctggccccac acaggcctaa ggaaccagca tggttcctgg ctaccgtggg ggtctctcct
     3841 gaccatcagg gcaaaggact gggaagtgct gtggtcctgc caggagtgga ggctgcagaa
     3901 cgagctggag tccctgcatt tctggagacc tctgctccac gaaacctgcc cttctatgaa
     3961 cggctgggct ttactgtgac cgcagatgtg gaggtccccg aaggacctag gacctggtgc
     4021 atgacacgca aacccggcgc ctgagcgatc gccgcggccg ccttcttaac ccaacagaag
     4081 gctcgagaag gtatattgct gttgacagtg agcgccaggc agatcccctt gtgagatagt
     4141 gaagccacag atgtatctca caaggggatc tgcctgatgc ctactgcctc ggacttcaag
     4201 gggctagaat tcggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4261 agggctaact gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4321 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4381 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4441 tttagtcagt gtggaaaatc tctagcaggg cccgtttcat gtgagcaaaa ggccagcaaa
     4501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5581 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     6301 cgggagatct cccgatccc
//
