LOCUS       sequence-343168-        4014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B.1.1.7 variant S-protein with c-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4014)
  TITLE     Spike variants with FLAG tags
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGGS-S-B.1.1.7-FLAG
FEATURES             Location/Qualifiers
     source          1..4014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(1475..1492)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             3988..4011
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      121 tccagcctcc ggactgagct ccttaaggtt aactctagag aattcggatc catgtttgtg
      181 ttcctggtgc tgctgccact ggtgtccagc cagtgtgtga acctgaccac caggacccaa
      241 cttcctcctg cctacaccaa ctccttcacc aggggagtct actaccctga caaggtgttc
      301 aggtcctctg tgctgcacag cacccaggac ctgttcctgc cattcttcag caatgtgacc
      361 tggttccatg ccatctctgg caccaatggc accaagaggt ttgacaaccc tgtgctgcca
      421 ttcaatgatg gagtctactt tgccagcaca gagaagagca acatcatcag gggctggatt
      481 tttggcacca ccctggacag caagacccag tccctgctga ttgtgaacaa tgccaccaat
      541 gtggtgatta aggtgtgtga gttccagttc tgtaatgacc cattcctggg agtctaccac
      601 aagaacaaca agtcctggat ggagtctgag ttcagggtct actcctctgc caacaactgt
      661 acctttgaat atgtgagcca accattcctg atggacttgg agggcaagca gggcaacttc
      721 aagaacctga gggagtttgt gttcaagaac attgatggct acttcaagat ttacagcaaa
      781 cacacaccaa tcaacctggt gagggacctg ccacagggct tctctgcctt ggaaccactg
      841 gtggacctgc caattggcat caacatcacc aggttccaga ccctgctggc tctgcacagg
      901 tcctacctga cacctggaga ctcctcctct ggctggacag caggagcagc agcctactat
      961 gtgggctacc tccaaccaag gaccttcctg ctgaaataca atgagaatgg caccatcaca
     1021 gatgctgtgg actgtgccct ggacccactg tctgagacca agtgtaccct gaaatccttc
     1081 acagtggaga agggcatcta ccagaccagc aacttcaggg tccaaccaac agagagcatt
     1141 gtgaggtttc caaacatcac caacctgtgt ccatttggag aggtgttcaa tgccaccagg
     1201 tttgcctctg tctatgcctg gaacaggaag aggattagca actgtgtggc tgactactct
     1261 gtgctctaca actctgcctc cttcagcacc ttcaagtgtt atggagtgag cccaaccaaa
     1321 ctgaatgacc tgtgtttcac caatgtctat gctgactcct ttgtgattag gggagatgag
     1381 gtgagacaga ttgcccctgg acaaacaggc aagattgctg actacaacta caaactgcct
     1441 gatgacttca caggctgtgt gattgcctgg aacagcaaca acctggacag caaggtggga
     1501 ggcaactaca actacctcta cagactgttc aggaagagca acctgaaacc atttgagagg
     1561 gacatcagca cagagattta ccaggctggc agcacaccat gtaatggagt ggagggcttc
     1621 aactgttact ttccactcca atcctatggc ttccaaccaa cctatggagt gggctaccaa
     1681 ccatacaggg tggtggtgct gtcctttgaa ctgctccatg cccctgccac agtgtgtgga
     1741 ccaaagaaga gcaccaacct ggtgaagaac aagtgtgtga acttcaactt caatggactg
     1801 acaggcacag gagtgctgac agagagcaac aagaagttcc tgccattcca acagtttggc
     1861 agggacattg atgacaccac agatgctgtg agggacccac agaccttgga gattctggac
     1921 atcacaccat gttcctttgg aggagtgtct gtgattacac ctggcaccaa caccagcaac
     1981 caggtggctg tgctctacca gggtgtgaac tgtactgagg tgcctgtggc tatccatgct
     2041 gaccaactta caccaacctg gagggtctac agcacaggca gcaatgtgtt ccagaccagg
     2101 gctggctgtc tgattggagc agagcatgtg aacaactcct atgagtgtga catcccaatt
     2161 ggagcaggca tctgtgcctc ctaccagacc cagaccaaca gccataggag ggcaaggtct
     2221 gtggcaagcc agagcatcat tgcctacaca atgagtctgg gagcagagaa ctctgtggct
     2281 tacagcaaca acagcattgc catcccaatc aacttcacca tctctgtgac cacagagatt
     2341 ctgcctgtga gtatgaccaa gacctctgtg gactgtacaa tgtatatctg tggagacagc
     2401 acagagtgta gcaacctgct gctccaatat ggctccttct gtacccaact taacagggct
     2461 ctgacaggca ttgctgtgga acaggacaag aacacccagg aggtgtttgc ccaggtgaag
     2521 cagatttaca agacacctcc aatcaaggac tttggaggct tcaacttcag ccagattctg
     2581 cctgacccaa gcaagccaag caagaggtcc ttcattgagg acctgctgtt caacaaggtg
     2641 accctggctg atgctggctt catcaagcaa tatggagact gtctgggaga cattgctgcc
     2701 agggacctga tttgtgccca gaagttcaat ggactgacag tgctgcctcc actgctgaca
     2761 gatgagatga ttgcccaata cacctctgcc ctgctggctg gcaccatcac ctctggctgg
     2821 acctttggag caggagcagc cctccaaatc ccatttgcta tgcagatggc ttacaggttc
     2881 aatggcattg gagtgaccca gaatgtgctc tatgagaacc agaaactgat tgccaaccag
     2941 ttcaactctg ccattggcaa gattcaggac tccctgtcca gcacagcctc tgccctgggc
     3001 aaactccaag atgtggtgaa ccagaatgcc caggctctga acaccctggt gaagcaactt
     3061 tccagcaact ttggagccat ctcctctgtg ctgaatgaca tcctggccag actggacaag
     3121 gtggaggctg aggtccagat tgacagactg attacaggca gactccaatc cctccaaacc
     3181 tatgtgaccc aacaacttat cagggctgct gagattaggg catctgccaa cctggctgcc
     3241 accaagatga gtgagtgtgt gctgggacaa agcaagaggg tggacttctg tggcaagggc
     3301 taccacctga tgagttttcc acagtctgcc cctcatggag tggtgttcct gcatgtgacc
     3361 tatgtgcctg cccaggagaa gaacttcacc acagcccctg ccatctgcca tgatggcaag
     3421 gctcactttc caagggaggg agtgtttgtg agcaatggca cccactggtt tgtgacccag
     3481 aggaacttct atgaaccaca gattatcacc acacacaaca cctttgtgtc tggcaactgt
     3541 gatgtggtga ttggcattgt gaacaacaca gtctatgacc cactccaacc tgaactggac
     3601 tccttcaagg aggaactgga caaatacttc aagaaccaca ccagccctga tgtggacctg
     3661 ggagacatct ctggcatcaa tgcctctgtg gtgaacatcc agaaggagat tgacagactg
     3721 aatgaggtgg ctaagaacct gaatgagtcc ctgattgacc tccaagaact gggcaaatat
     3781 gaacaataca tcaagtggcc atggtacatc tggctgggct tcattgctgg actgattgcc
     3841 attgtgatgg tgaccatcat gctgtgctgc atgacctcat gttgctcctg tcttaagggg
     3901 tgctgctcct gcggttcctg ttgcaaattt gacgaggacg acagcgaacc ggtgcttaag
     3961 ggtgtgaagc tgcattacac cggaggcgat tacaaggatg acgatgacaa gtga
//
