LOCUS       sequence-343171-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human MDA5 S88D mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Guney MH, Nagalekshmi K, McCauley SM, Carbone C, Aydemir O, Luban J
  TITLE     IFIH1 (MDA5) is required for innate immune detection of 
            intron-containing RNA expressed from the HIV-1 provirus.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 16;121(29):e2404349121. doi: 
            10.1073/pnas.2404349121. Epub 2024 Jul 10.
  PUBMED    38985764
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-16"; volume: "121(29):e2404349121. 
            doi"; pages: " 10.1073/pnas.2404349121. Epub 2024 Jul 10"
COMMENT     Sequence Label: pscALPSpuro-MDA5-S88D
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1067..1300
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1484..1528
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1826..1943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2080..2487
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6130..6729
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6130..6149)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6626..6646
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6739..7327
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6792..6812)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7210..7221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7845..8025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8096..8684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8176..8195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8855..9646,9647..9715))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9478..9497
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9716..9820)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9895..9914)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10088..10108)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10093..10422
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10273..10408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10335..10354
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        10434..10813
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
      961 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1021 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1081 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1141 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1201 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1261 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1321 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1381 aacagatttg gaatcacacg acctggatgg agtgggacag agaattaaca attacacaag
     1441 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1501 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1561 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1621 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1681 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     1741 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     1801 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     1861 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     1921 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     1981 taattaactg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2041 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2101 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2161 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2221 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2281 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2341 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2401 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2461 gtcctccgac agactgagtc gcccgggggt ctagaagcgc tggatccatg agtaatggtt
     2521 actcaaccga tgagaatttc aggtatctga tttcctgttt cagagcccga gtcaaaatgt
     2581 acatacaagt cgaacctgtg ttggattatc tcacttttct cccagcagaa gtgaaggaac
     2641 aaattcaacg gacggtagca acttcaggaa atatgcaggc ggttgagctt ttactcagca
     2701 cattggaaaa gggtgtgtgg caccttgggt ggactagaga gtttgtagag gccctccgcc
     2761 gcactggtga ccccttggct gcgcgataca tgaacccgga gctgaccgat ttgccatcac
     2821 cttcctttga gaacgcccat gatgagtatc tgcaactctt gaaccttctt caaccgacct
     2881 tggttgacaa actgttggta cgcgacgtac tggacaaatg tatggaggaa gaactgctga
     2941 ccattgaaga caggaatcgg atcgccgccg ccgagaataa cggcaatgaa tccggcgtca
     3001 gggaattgct gaaacgcatc gttcaaaagg agaactggtt tagtgccttt ctcaacgtgc
     3061 tgcgccaaac cggtaataat gagttggtac aagagttgac gggttccgac tgttccgagt
     3121 caaatgcgga aatagaaaac ctttctcaag tagacggccc gcaggtcgag gaacagcttc
     3181 tgagtacgac agtccaacca aatctggaga aggaggtgtg gggaatggaa aataacagtt
     3241 ctgaatcaag tttcgccgac agttcagtcg tgtccgaatc tgacacgagc ttggcagaag
     3301 gatcagttag ttgtttggac gagtctcttg ggcacaactc caacatgggc agcgactctg
     3361 ggactatggg tagcgactct gatgaggaaa acgtggccgc tcgggcgtcc ccagagcctg
     3421 aacttcagct tcgcccctat cagatggagg ttgcgcagcc cgccttggaa ggtaaaaaca
     3481 tcattatctg tttgcctacc ggcagtggaa agacacgagt agcggtctac atcgccaaag
     3541 accacttgga caaaaagaaa aaggcgtcag agcctggtaa agtgatagtt ttggtcaaca
     3601 aggttctctt ggtggagcag ctctttcgga aggagtttca gccctttctg aagaaatggt
     3661 atcgagtgat cggcttgtca ggcgatacgc agttgaaaat atcatttcca gaggtagtaa
     3721 agtcctgtga catcatcata tcaactgccc aaattcttga aaattccttg ctgaacttgg
     3781 aaaacgggga agacgcgggg gtacaactca gcgatttcag tctgatcatc attgatgagt
     3841 gtcatcacac gaataaagaa gcagtgtata acaatataat gaggcactat ctgatgcaga
     3901 aacttaaaaa caacagactg aaaaaggaga acaagcccgt cattccattg ccgcaaattc
     3961 ttggccttac ggccagcccc ggcgttggtg gggccaccaa acaagccaag gctgaagaac
     4021 acatcctcaa attgtgcgca aacttggatg cttttacaat aaagacagtg aaagaaaatc
     4081 ttgaccagct caaaaaccag atacaagaac cttgcaagaa attcgcgata gccgacgcta
     4141 cccgagaaga tccttttaaa gagaagctgc tggaaattat gacgcggata cagacgtatt
     4201 gtcaaatgag ccctatgtct gactttggga cgcaaccata tgagcaatgg gccatacaga
     4261 tggaaaaaaa agctgcgaaa gagggcaatc gaaaagagcg cgtctgtgcg gagcatctca
     4321 ggaagtataa tgaggcgctg cagatcaacg atacgatccg catgatcgat gcgtacactc
     4381 acctggaaac gttttacaat gaagagaaag ataagaaatt tgcggtcatc gaggacgact
     4441 cagatgaagg cggtgacgac gagtattgcg acggagatga ggacgaggat gatcttaaga
     4501 agcctttgaa actcgatgag actgaccgct tcctcatgac ccttttcttc gagaataata
     4561 aaatgctgaa gaggctggcc gaaaatccag agtacgagaa cgagaagctc acgaaactgc
     4621 gcaatacaat aatggaacaa tatacccgca cggaagaaag cgcaagaggc atcattttca
     4681 ctaaaaccag gcagagtgcc tacgccttga gtcagtggat aaccgaaaat gagaaattcg
     4741 ctgaggtcgg tgtaaaagct caccatctta tcggcgctgg acacagttca gagtttaaac
     4801 cgatgactca aaatgagcag aaagaggtaa ttagtaaatt ccgaacagga aagattaact
     4861 tgctgatagc cacaactgtg gccgaagagg gcttggacat aaaggagtgc aatatcgtca
     4921 tacggtatgg tctcgtcact aacgaaatag caatggtcca ggcccgcgga cgggctagag
     4981 ccgatgagag cacttatgtg cttgttgccc attctggaag cggggtgatc gagcatgaaa
     5041 ccgtgaatga ttttcgcgaa aaaatgatgt acaaggctat acactgtgtg caaaacatga
     5101 agccagaaga atacgcccac aagattctgg aactccaaat gcaaagcatt atggagaaaa
     5161 aaatgaagac taaacgcaac attgcgaaac attacaagaa caatccatct ctgattacat
     5221 ttctttgtaa gaactgctcc gtgctggcct gttctggaga ggacattcac gtaatagaga
     5281 aaatgcacca cgtgaatatg actcctgagt ttaaggagct ttacatagtt cgagagaaca
     5341 aggcgctcca aaagaagtgt gcagattatc aaataaacgg agaaatcatt tgtaagtgtg
     5401 gtcaagcgtg gggaaccatg atggtgcaca aaggcctcga tcttccctgc ttgaaaataa
     5461 gaaattttgt agttgtattc aaaaacaact ccactaaaaa acaatataag aaatgggtag
     5521 aactgccaat tacgttccct aacttggact atagtgaatg ttgtctgttt agtgacgaag
     5581 attaggcggc cgcccagcac agtggctcga gccgcgggtt aactggccag aattctcgac
     5641 cttgactggc ggcgcgacct tgaggcctgc gttcgcctca gttgccccct ctgtgcaatg
     5701 gggagacgcg cctcatcgct tgacaacggc cgaagagccg ccgcgcttcc gtctcccgcg
     5761 tgcgcgcgcc atgctgccca cccccgttcc gcactgaccc tcccccgtgc cccgcgtccc
     5821 gtactgccgc cccgccccga gtcccatgcc gcagccaccg cgacggagcc cgcaggcggg
     5881 aacctgcctc cgcgcgttag cgcgcacgcg cgcctcatgt gtcgtcccca tcagcgccgg
     5941 cttccgtcta taggccagat gcactgtcac tctggcgaag tcgcagaccc gattggccgg
     6001 gacggaggcg cgagaccggg ttgcgggcgg ggccgaacgt ggtataaaag gggcgggagg
     6061 ccaggctcgt gccgttttgc agacgccacc gccgaggaaa accgtgtact attagccacg
     6121 cgtgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6181 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6241 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6301 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6361 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6421 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6481 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6541 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6601 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6661 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6721 ggtgcctgag cgatcgctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6901 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6961 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7021 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7081 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     7141 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7201 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7261 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7321 tccccgctta atcgcgtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     7381 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     7441 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7501 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     7561 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     7621 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7681 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     7741 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     7801 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     7861 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7921 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7981 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttcatgt
     8041 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8101 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8161 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8221 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8281 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8341 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8401 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8461 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8521 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8581 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8641 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8701 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8761 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8821 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8881 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8941 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9001 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9061 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9121 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9181 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9241 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9301 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9361 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9421 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9481 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9541 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9601 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9661 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9721 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9781 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9841 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     9901 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9961 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
    10021 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    10081 gcgctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10141 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10201 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10261 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10321 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
    10381 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcgc gttgacattg
    10441 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    10501 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    10561 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    10621 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    10681 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    10741 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
    10801 cgctattacc atg
//
