LOCUS       sequence-343180-        3730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric 
            intron and the Venus cDNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Mauduit D, Taskiran II, Minnoye L, de Waegeneer M, Christiaens V, 
            Hulselmans G, Demeulemeester J, Wouters J, Aerts S
  TITLE     Analysis of long and short enhancers in melanoma cell states.
  JOURNAL   Elife. 2021 Dec 7;10. pii: 71735. doi: 10.7554/eLife.71735.
  PUBMED    34874265
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-12-7"; volume: "10. pii: 71735. doi"; pages: " 
            10.7554/eLife.71735"
COMMENT     Sequence Label: pSA293-CHEQ-seq
FEATURES             Location/Qualifiers
     source          1..3730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          188..417
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     400..419
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(475..477,478..480,481..1191)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(520..541)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(781..800)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1128..1149
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1234..1355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1245..1264)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1299..1318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    1595..1664
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1685..1702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1856..2444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1936..1955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2644..3435,3436..3504))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3609..3657
                     /note="synthetic polyadenylation signal"
     misc_feature    join(3671..3730,1..32)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3711..3730
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctccc
       61 tagggtactt atataagggg gtgggggcgc gttcgtcctc agtcgcgatc gaacactcga
      121 gccgagcaga cgtgcctacg gaccggggcc cgaattaatt cgctgtctgc gagggccagc
      181 tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt
      241 tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc
      301 gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt
      361 gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc tccacaggtg
      421 tccactccca ggtccaactg caggtcgcct gcaggcttaa accggtcaga aaaaatggtg
      481 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      541 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      601 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      661 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      721 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      781 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      841 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      901 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      961 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     1021 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1081 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1141 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gttatctaga
     1201 gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     1261 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     1321 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     1381 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     1441 gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc
     1501 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     1561 atgcaactcg taggacaggt gccggcatgg accagatggg tgaggtggag tacgcgcccg
     1621 gggagcccaa gggcacgccc tggcacccgc accgcggctt cgagaccgag cgctcttccg
     1681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2281 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2581 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
     2641 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
     2701 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
     2761 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
     2821 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
     2881 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
     2941 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
     3001 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
     3061 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
     3121 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
     3181 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
     3241 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
     3301 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
     3361 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
     3421 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
     3481 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
     3541 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
     3601 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     3661 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     3721 aggctgtccc
//
