LOCUS       sequence-343181-        3849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This gene was cloned into the gateway middle entry vector. It can be
            used for generating antisense riboprobe or gene expression using a 
            gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Silic MR, Murata SH, Park SJ, Zhang G
  TITLE     Evolution of inwardly rectifying potassium channels and their gene 
            expression in zebrafish embryos.
  JOURNAL   Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425.
  PUBMED    34558132
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Sep 23. doi: 10.1002/dvdy.425."
COMMENT     Sequence Label: pDONR221-kcnj11l
FEATURES             Location/Qualifiers
     source          1..3849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1974..2073)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2090..2109)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2091..2109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2114..2130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2114..2130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2232..2251)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2243..3052
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2310..2329)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3199..3787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3688..3707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc accatgttgg cccgcaaagg tcttttgcct gatgggttct tgttaacgcg
      721 actcgctgaa gatgcacaac ccagccgcat ccggccaaag tcacaaaggg cacgttttat
      781 caccaagagc ggctcatgta atgtagccca caagaatatt cgagaacagg gtcggtttct
      841 ccaagatgtg ttcaccacca tggtggacct gaaatggcag cattctttgc tcatcttcac
      901 ctcagcgttc ctctgctcct ggatgctctt tgccatggtg tggtggctcc tggcattcgc
      961 tcatggagat ctagaacctc gagatcccaa tgaacccgga cctgtgccgt gcgtcacatc
     1021 cattcactcc ttcacttctg cttttctgtt ctccattgaa gtgcaggtaa ctattggttt
     1081 cggaggacga atggtgacag aggagtgtcc gctggccatc atagtcctca tcatccagaa
     1141 cattctgggc ctgatcatca acgccatcat gcttggctgt gtgttcatga agacagctca
     1201 agccaaccgc agagcagaaa ccctcatctt ctcccgcaac gcagtcattg ccccaaggaa
     1261 tggcagaccg acattgatgt ttcgtgttgg agatctgaga aaaagcatga tcatctctgc
     1321 caacatacag ctgcaggtta ttcggcggac agtgacagcg gagggcgaag tgattcccgt
     1381 ctgtcagtta gatatccagg tggaaaaccc tctcaggagc aacggcatat tcctcgtctc
     1441 tccactcatt atcagtcaca ccattgacag agggagtcca ctgtacgagc tctcagcgca
     1501 gtcaatggct accgaagatc tggagatcat tgtcatttta gaaggtgttg tggagacgac
     1561 tggcatcaca atgcaggccc gcacatccta cataccagaa gagatcttgt ggggtcggcg
     1621 tttcgtctct attatgacag aggaggatgg acgctactct gtcgactact ccaaatttgg
     1681 aaacactgtt cctgtacgga tgccagcgct cagtgctaag gagctggacc aaaccagggg
     1741 cgtgcaggac agcggacccc atgagggtaa acctcagggc tgggggctgg tgagggcggg
     1801 acgaggtggg tacaggaggg gtggagcaag ggcatgtgat gacacggcag caaagccatg
     1861 gtacgtgcca gcggagaaag aggaggagaa gaagggtcaa aagaaaaccg tgaagcttga
     1921 ggagatagga agagagattg aagaagagac aactgaggat atgagcgatt aggacccagc
     1981 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     2041 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2101 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2161 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     2221 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     2281 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     2341 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2401 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2461 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2521 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     2581 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2641 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2701 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2761 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2821 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2881 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2941 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     3001 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     3061 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     3121 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     3181 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3241 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3301 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3361 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3421 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3481 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3541 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3601 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3721 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3781 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3841 tcacatgtt
//
