LOCUS       sequence-343213-       10242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10242)
  TITLE     Sun Lab Overexpression Plasmids
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLXSN(GFP)-hp100
FEATURES             Location/Qualifiers
     source          1..10242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..155
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        300..679
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        680..883
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     834..854
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             884..1059
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1122..1321
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1522..1938
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1868..1890
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1906..1922
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4803..4823)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4808..5137
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4988..5123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5196..5198,5199..5201,5202..5915)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5196..5198,5199..5201,5202..5912)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5241..5262)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5502..5521)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5849..5870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6021..6614
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     intron          6959..7024
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7154..7174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7446..7580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7483..7502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7537..7556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8038..8172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8237..8254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8408..8996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8488..8507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9167..9958,9959..10027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9790..9809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10028..10132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10200..10218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
       61 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
      121 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
      181 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
      241 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcgcgttg
      301 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      361 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      421 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      481 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      541 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      601 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      661 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      721 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      781 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      841 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca
      901 acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc
      961 aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc
     1021 ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg
     1081 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
     1141 ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag
     1201 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc
     1261 ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga
     1321 ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag
     1381 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc
     1441 gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
     1501 ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt
     1561 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
     1621 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
     1681 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
     1741 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
     1801 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
     1861 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
     1921 actccttctc taggcgccgg aattcagatc tggtaccacg cgtatcgata agcttaccat
     1981 ggagagttgc tacaacccag gtctggatgg tattattgaa tatgatgatt tcaaattgaa
     2041 ctcctccatt gtggaaccca aggagccagc cccagaaaca gctgatggcc cctacctggt
     2101 gatcgtggaa cagcctaagc agagaggctt ccgatttcga tatggctgtg aaggcccctc
     2161 ccatggagga ctgcccggtg cctccagtga gaagggccga aagacctatc ccactgtcaa
     2221 gatctgtaac tacgagggac cagccaagat cgaggtggac ctggtaacac acagtgaccc
     2281 acctcgtgct catgcccaca gtctggtggg caagcaatgc tcggagctgg ggatctgcgc
     2341 cgtttctgtg gggcccaagg acatgactgc ccaatttaac aacctgggtg tcctgcatgt
     2401 gactaagaag aacatgatgg ggactatgat acaaaaactt cagaggcagc ggctccgctc
     2461 taggccccag ggccttacgg aggccgagca gcgggagctg gagcaagagg ccaaagaact
     2521 gaagaaggtg atggatctga gtatagtgcg gctgcgcttc tctgccttcc ttagagccag
     2581 tgatggctcc ttctccctgc ccctgaagcc agtcatctcc cagcccatcc atgacagcaa
     2641 atctccgggg gcatcaaacc tgaagatttc tcgaatggac aagacagcag gctctgtgcg
     2701 gggtggagat gaagtttatc tgctttgtga caaggtgcag aaagatgaca ttgaggttcg
     2761 gttctatgag gatgatgaga atggatggca ggcctttggg gacttctctc ccacagatgt
     2821 gcataaacag tatgccattg tgttccggac acccccctat cacaagatga agattgagcg
     2881 gcctgtaaca gtgtttctgc aactgaaacg caagcgagga ggggacgtgt ctgattccaa
     2941 acagttcacc tattaccctc tggtggaaga caaggaagag gtgcagcgga agcggaggaa
     3001 ggccttgccc accttctccc agcccttcgg gggtggctcc cacatgggtg gaggctctgg
     3061 gggtgcagcc gggggctacg gaggagctgg aggaggtggc agcctcggtt tcttcccctc
     3121 ctccctggcc tacagcccct accagtccgg cgcgggcccc atgggctgct acccgggagg
     3181 cgggggcggg gcgcagatgg ccgccacggt gcccagcagg gactccgggg aggaagccgc
     3241 ggagccgagc gccccctcca ggacccccca gtgcgagccg caggccccgg agatgctgca
     3301 gcgagctcga gagtacaacg cgcgcctgtt cggcctggcg cagcgcagcg cccgagccct
     3361 actcgactac ggcgtcaccg cggacgcgcg cgcgctgctg gcgggacagc gccacctgct
     3421 gacggcgcag gacgagaacg gagacacacc actgcaccta gccatcatcc acgggcagac
     3481 cagtgtcatt gagcagatag tctatgtcat ccaccacgcc caggacctcg gcgttgtcaa
     3541 cctcaccaac cacctgcacc agacgcccct gcacctggcg gtgatcacgg ggcagacgag
     3601 tgtggtgagc tttctgctgc gggtaggtgc agacccagct ctgctggatc ggcatggaga
     3661 ctcagccatg catctggcgc tgcgggcagg cgctggtgct cctgagctgc tgcgtgcact
     3721 gcttcagagt ggagctcctg ctgtgcccca gctgttgcat atgcctgact ttgagggact
     3781 gtatccagta cacctggcgg tccgagcccg aagccctgag tgcctggatc tgctggtgga
     3841 cagtggggct gaagtggagg ccacagagcg gcagggggga cgaacagcct tgcatctagc
     3901 cacagagatg gaggagctgg ggttggtcac ccatctggtc accaagctcc gggccaacgt
     3961 gaacgctcgc acctttgcgg gaaacacacc cctgcacctg gcagctggac tggggtaccc
     4021 gaccctcacc cgcctccttc tgaaggctgg tgctgacatc catgctgaaa acgaggagcc
     4081 cctgtgccca ctgccttcac cccctacctc tgatagcgac tcggactctg aagggcctga
     4141 gaaggacacc cgaagcagct tccggggcca cacgcctctt gacctcactt gcagcaccaa
     4201 ggtgaagacc ttgctgctaa atgctgctca gaacaccatg gagccacccc tgaccccgcc
     4261 cagcccagca gggccgggac tgtcacttgg tgatacagct ctgcagaacc tggagcagct
     4321 gctagacggg ccagaagccc agggcagctg ggcagagctg gcagagcgtc tggggctgcg
     4381 cagcctggta gacacgtacc gacagacaac ctcacccagt ggcagcctcc tgcgcagcta
     4441 cgagctggct ggcggggacc tggcaggtct actggaggcc ctgtctgaca tgggcctaga
     4501 ggagggagtg aggctgctga ggggtccaga aacccgagac aagctgccca gcacagaggt
     4561 gaaggaagac agtgcgtacg ggagccagtc agtggagcag gaggcagaga agctgggccc
     4621 accccctgag ccaccaggag ggctctgcca cgggcacccc cagcctcagg tgcactgacc
     4681 tgctgcctgc ccccagcccc cttcccggac cccctgtaca gcgtccccac ctatttcaaa
     4741 tcttatttaa caccccacac ccacccctca gttgggacaa ataatctaga ggatccggct
     4801 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4861 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4921 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4981 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5041 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5101 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgggctgc aggtcgaggc
     5161 ggatctgatc aagagacagg atgagcatcg ataccatggt gagcaagggc gaggagctgt
     5221 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     5281 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     5341 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     5401 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     5461 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     5521 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     5581 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     5641 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     5701 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5761 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     5821 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5881 ggatcactct cggcatggac gagctgtaca agtaagatgc atcgcatcgc cttctatcgc
     5941 cttcttgacg agttcttctg agcgggactc tggggttcga taaaataaaa gattttattt
     6001 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     6061 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     6121 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     6181 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     6241 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     6301 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     6361 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     6421 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     6481 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     6541 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     6601 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
     6661 cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6721 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6781 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     6841 tcacgtagcg atagcggagt gtatctagct aggtagctag aggatctttg tgaaggaacc
     6901 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     6961 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     7021 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     7081 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     7141 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     7201 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     7261 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat
     7321 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     7381 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     7441 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7501 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7561 tatcttatca tgtctggatc agcttcagaa gatggcggag ggcctccaac acagtaattt
     7621 tcctcccgac tcttaaaata gaaaatgtca agtcagttaa gcaggaagtg actaactgac
     7681 cgcagctggc cgtgcgacat cctcttttaa ttagttgcta ggcaactgcc ctccagaggg
     7741 cagtgtggtt ttgcaagagg aagcaaaaag cctctccacc caggcctaga atgtttccac
     7801 ccaatcatta ctatgacaac agctgttttt tttagtatta agcagaggcc gggggcccct
     7861 ggcctccgct tactctggag aaaaagaaga gaggcattgt agaggcttcc agaggcaact
     7921 tgtcaaaaca ggactgcttc tatttctgtc acactgtctg gccctgtcac aaggtccagc
     7981 acctccatac cccctttaat aagcagtttg ggaacgggtg cgggtcttac tccgcccatc
     8041 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8101 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8161 ttttttggag gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8821 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9481 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10141 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    10201 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tg
//
