LOCUS       sequence-343215-        7710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7710)
  TITLE     Sun Lab Overexpression Plasmids
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLXSN(GFP)-hp100 SS/AA
FEATURES             Location/Qualifiers
     source          1..7710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..68
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     60..77
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     61..77
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(3774..3790)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3774..3790)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    3798..4420
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3997..4016)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4444..4464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4449..4778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4629..4764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4817..4913
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(4989..5008)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4990..5008)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5022..5038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5022..5038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5035..5057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5046..5062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5070..5076,5077..5094,5095..5100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5115..5136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5253..5270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5424..6012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5504..5523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6183..6974,6975..7043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6806..6825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7044..7148)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7175..7630
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7262..7281)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7472..7493
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
       61 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
      121 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      181 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      841 attgttttta tttttaattt tctttcaaat acttccatcg aattcagatc tggtaccacg
      901 cgtatcgata agcttcacca tggagagttg ctacaaccca ggtctggatg gtattattga
      961 atatgatgat ttcaaattga actcctccat tgtggaaccc aaggagccag ccccagaaac
     1021 agctgatggc ccctacctgg tgatcgtgga acagcctaag cagagaggct tccgatttcg
     1081 atatggctgt gaaggcccct cccatggagg actgcccggt gcctccagtg agaagggccg
     1141 aaagacctat cccactgtca agatctgtaa ctacgaggga ccagccaaga tcgaggtgga
     1201 cctggtaaca cacagtgacc cacctcgtgc tcatgcccac agtctggtgg gcaagcaatg
     1261 ctcggagctg gggatctgcg ccgtttctgt ggggcccaag gacatgactg cccaatttaa
     1321 caacctgggt gtcctgcatg tgactaagaa gaacatgatg gggactatga tacaaaaact
     1381 tcagaggcag cggctccgct ctaggcccca gggccttacg gaggccgagc agcgggagct
     1441 ggagcaagag gccaaagaac tgaagaaggt gatggatctg agtatagtgc ggctgcgctt
     1501 ctctgccttc cttagagcca gtgatggctc cttctccctg cccctgaagc cagtcatctc
     1561 ccagcccatc catgacagca aatctccggg ggcatcaaac ctgaagattt ctcgaatgga
     1621 caagacagca ggctctgtgc ggggtggaga tgaagtttat ctgctttgtg acaaggtgca
     1681 gaaagatgac attgaggttc ggttctatga ggatgatgag aatggatggc aggcctttgg
     1741 ggacttctct cccacagatg tgcataaaca gtatgccatt gtgttccgga caccccccta
     1801 tcacaagatg aagattgagc ggcctgtaac agtgtttctg caactgaaac gcaagcgagg
     1861 aggggacgtg tctgattcca aacagttcac ctattaccct ctggtggaag acaaggaaga
     1921 ggtgcagcgg aagcggagga aggccttgcc caccttctcc cagcccttcg ggggtggctc
     1981 ccacatgggt ggaggctctg ggggtgcagc cgggggctac ggaggagctg gaggaggtgg
     2041 cagcctcggt ttcttcccct cctccctggc ctacagcccc taccagtccg gcgcgggccc
     2101 catgggctgc tacccgggag gcgggggcgg ggcgcagatg gccgccacgg tgcccagcag
     2161 ggactccggg gaggaagccg cggagccgag cgccccctcc aggacccccc agtgcgagcc
     2221 gcaggccccg gagatgctgc agcgagctcg agagtacaac gcgcgcctgt tcggcctggc
     2281 gcagcgcagc gcccgagccc tactcgacta cggcgtcacc gcggacgcgc gcgcgctgct
     2341 ggcgggacag cgccacctgc tgacggcgca ggacgagaac ggagacacac cactgcacct
     2401 agccatcatc cacgggcaga ccagtgtcat tgagcagata gtctatgtca tccaccacgc
     2461 ccaggacctc ggcgttgtca acctcaccaa ccacctgcac cagacgcccc tgcacctggc
     2521 ggtgatcacg gggcagacga gtgtggtgag ctttctgctg cgggtaggtg cagacccagc
     2581 tctgctggat cggcatggag actcagccat gcatctggcg ctgcgggcag gcgctggtgc
     2641 tcctgagctg ctgcgtgcac tgcttcagag tggagctcct gctgtgcccc agctgttgca
     2701 tatgcctgac tttgagggac tgtatccagt acacctggcg gtccgagccc gaagccctga
     2761 gtgcctggat ctgctggtgg acagtggggc tgaagtggag gccacagagc ggcagggggg
     2821 acgaacagcc ttgcatctag ccacagagat ggaggagctg gggttggtca cccatctggt
     2881 caccaagctc cgggccaacg tgaacgctcg cacctttgcg ggaaacacac ccctgcacct
     2941 ggcagctgga ctggggtacc cgaccctcac ccgcctcctt ctgaaggctg gtgctgacat
     3001 ccatgctgaa aacgaggagc ccctgtgccc actgccttca ccccctacct ctgatagcga
     3061 ctcggactct gaagggcctg agaaggacac ccgaagcagc ttccggggcc acacgcctct
     3121 tgacctcact tgcagcacca aggtgaagac cttgctgcta aatgctgctc agaacaccat
     3181 ggagccaccc ctgaccccgc ccagcccagc agggccggga ctgtcacttg gtgatacagc
     3241 tctgcagaac ctggagcagc tgctagacgg gccagaagcc cagggcagct gggcagagct
     3301 ggcagagcgt ctggggctgc gcagcctggt agacacgtac cgacagacaa cctcacccag
     3361 tggcagcctc ctgcgcagct acgagctggc tggcggggac ctggcaggtc tactggaggc
     3421 cctgtctgac atgggcctag aggagggagt gaggctgctg aggggtccag aaacccgaga
     3481 caagctgccc agcacagagg tgaaggaaga cgctgcgtac ggcgcccagt cagtggagca
     3541 ggaggcagag aagctgggcc caccccctga gccaccagga gggctctgcc acgggcaccc
     3601 ccagcctcag gtgcactgac ctgctgcctg cccccagccc ccttcccgga ccccctgtac
     3661 agcgtcccca cctatttcaa atcttattta acaccccaca cccacccctc agttgggaca
     3721 aataaaggat tctcatggga aggggaggac cccggaattc ctgcagcccg ggggatccac
     3781 tagttctaga ggatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     3841 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     3901 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     3961 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     4021 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     4081 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     4141 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     4201 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     4261 ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc
     4321 ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc
     4381 tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc
     4441 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     4501 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4621 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4681 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4741 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa
     4801 accagaaagt taactggtaa gtttagtctt tttgtctttt atttcaggtc ccggatccgg
     4861 tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta
     4921 cggaagtgtt acttctgctc taaaagctgc tgcaggtcga ctctagggcc gcgtaccgag
     4981 ctcgaattcc ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg
     5041 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5101 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5161 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5221 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5281 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5341 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5401 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5461 aatcgacgct caagtcagag gtggcgaaac ccgataggac tataaagata ccaggcgttt
     5521 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5581 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5641 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5701 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5761 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5821 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5881 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5941 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6001 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6061 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6181 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6241 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6301 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6361 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6421 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6481 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6541 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6601 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6661 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6721 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6781 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6841 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6901 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6961 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7021 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7081 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7141 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     7201 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7261 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7321 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7381 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7441 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7501 ctcaaccata tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7561 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7621 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     7681 cctcttcgct attacgccag ctggcgaaag
//
