LOCUS       sequence-343218-       11283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Li Y, Yang JY, Xie X, Jie Z, Zhang L, Shi J, Lin D, Gu M, Zhou X, Li
            HS, Watowich SS, Jain A, Yun Jung S, Qin J, Cheng X, Sun SC
  TITLE     Preventing abnormal NF-kappaB activation and autoimmunity by 
            Otub1-mediated p100 stabilization.
  JOURNAL   Cell Res. 2019 Jun;29(6):474-485. doi: 10.1038/s41422-019-0174-3. 
            Epub 2019 May 13.
  PUBMED    31086255
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-019-0174-3"; journalName: "Cell Res"; date: 
            "2019-06"; volume: "29"; issue: "6"; pages: "474-485"
COMMENT     Sequence Label: pCLXSN(GFP)-myc-BioID-hp100
FEATURES             Location/Qualifiers
     source          1..11283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        191..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        571..774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     725..745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             775..950
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1013..1212
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1413..1829
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1759..1781
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1797..1813
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1837..1846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1846..1875
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1879..2226,2227..2229,2230..2838)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5735..5755)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5740..6069
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5920..6055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(6128..6130,6131..6133,6134..6847)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6128..6130,6131..6133,6134..6844)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6173..6194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6434..6453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6781..6802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6953..7546
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     intron          7891..7956
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8086..8106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8378..8512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8415..8434)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8469..8488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8970..9104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9169..9186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9340..9928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9420..9439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10099..10890,10891..10959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10722..10741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10960..11064)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11132..11150
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
       61 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      121 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      181 ccagcgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      541 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcaataa
      781 aagagcccac aacccctcac tcggcgcgcc agtcttccga tagactgcgt cgcccgggta
      841 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggtctc gctgttcctt
      901 gggagggtct cctctgagtg attgactacc cacgacgggg gtctttcatt tgggggctcg
      961 tccgggattt ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     1021 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt atgcgcctgc
     1081 gtctgtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     1141 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     1201 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     1261 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     1321 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     1381 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     1441 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1501 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1561 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1621 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1681 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1741 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1801 atccagccct cactccttct ctaggcgccg gaattggcca ccatggaaca aaaactcatc
     1861 tcagaagagg atctcgacaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc
     1921 aacggcgagt tccactctgg cgagcagctg ggagagaccc tgggaatgag cagagccgcc
     1981 atcaacaagc acatccagac actgagagac tggggagtgg acgtgttcac cgtgcctggc
     2041 aagggctaca gcctgcctga gcctatccag ctgctgaacg ccaagcagat cctgggacag
     2101 ctggatggcg gaagcgtggc cgtgctgcct gtgatcgact ccaccaatca gtacctgctg
     2161 gacagaatcg gagagctgaa gtccggcgac gcctgcatcg ccgagtacca gcaggctggc
     2221 agaggaggca gaggacggaa gtggttcagc ccattcggag ccaacctgta cctgtccatg
     2281 ttctggagac tggagcaggg acctgctgct gccatcggac tgagtctggt gatcggaatc
     2341 gtgatggccg aggtgctgag aaagctggga gccgacaagg tgagagtgaa gtggcctaat
     2401 gacctgtacc tccaggaccg caagctggct ggcatcctgg tggagctgac aggcaagaca
     2461 ggcgatgccg ctcagatcgt gatcggagcc ggaatcaaca tggccatgag aagagtggag
     2521 gagagcgtgg tgaaccaggg ctggatcacc ctgcaggagg ctggcatcaa cctggaccgg
     2581 aacaccctgg ccgccatgct gatcagagag ctgagagccg ctctggagct gttcgagcag
     2641 gagggactgg ctccttacct gagcagatgg gagaagctgg acaacttcat caacagacct
     2701 gtgaagctga tcatcggcga caaggaaatc ttcggcatct ccagaggaat cgacaagcag
     2761 ggagctctgc tgctggagca ggacggaatc atcaagccct ggatgggcgg agaaatctcc
     2821 ctgagaagcg cagagaagct cgagcggccg ccactgtgct ggatatctgc agaattcaga
     2881 tctggtacca cgcgtatcga taagcttacc atggagagtt gctacaaccc aggtctggat
     2941 ggtattattg aatatgatga tttcaaattg aactcctcca ttgtggaacc caaggagcca
     3001 gccccagaaa cagctgatgg cccctacctg gtgatcgtgg aacagcctaa gcagagaggc
     3061 ttccgatttc gatatggctg tgaaggcccc tcccatggag gactgcccgg tgcctccagt
     3121 gagaagggcc gaaagaccta tcccactgtc aagatctgta actacgaggg accagccaag
     3181 atcgaggtgg acctggtaac acacagtgac ccacctcgtg ctcatgccca cagtctggtg
     3241 ggcaagcaat gctcggagct ggggatctgc gccgtttctg tggggcccaa ggacatgact
     3301 gcccaattta acaacctggg tgtcctgcat gtgactaaga agaacatgat ggggactatg
     3361 atacaaaaac ttcagaggca gcggctccgc tctaggcccc agggccttac ggaggccgag
     3421 cagcgggagc tggagcaaga ggccaaagaa ctgaagaagg tgatggatct gagtatagtg
     3481 cggctgcgct tctctgcctt ccttagagcc agtgatggct ccttctccct gcccctgaag
     3541 ccagtcatct cccagcccat ccatgacagc aaatctccgg gggcatcaaa cctgaagatt
     3601 tctcgaatgg acaagacagc aggctctgtg cggggtggag atgaagttta tctgctttgt
     3661 gacaaggtgc agaaagatga cattgaggtt cggttctatg aggatgatga gaatggatgg
     3721 caggcctttg gggacttctc tcccacagat gtgcataaac agtatgccat tgtgttccgg
     3781 acacccccct atcacaagat gaagattgag cggcctgtaa cagtgtttct gcaactgaaa
     3841 cgcaagcgag gaggggacgt gtctgattcc aaacagttca cctattaccc tctggtggaa
     3901 gacaaggaag aggtgcagcg gaagcggagg aaggccttgc ccaccttctc ccagcccttc
     3961 gggggtggct cccacatggg tggaggctct gggggtgcag ccgggggcta cggaggagct
     4021 ggaggaggtg gcagcctcgg tttcttcccc tcctccctgg cctacagccc ctaccagtcc
     4081 ggcgcgggcc ccatgggctg ctacccggga ggcgggggcg gggcgcagat ggccgccacg
     4141 gtgcccagca gggactccgg ggaggaagcc gcggagccga gcgccccctc caggaccccc
     4201 cagtgcgagc cgcaggcccc ggagatgctg cagcgagctc gagagtacaa cgcgcgcctg
     4261 ttcggcctgg cgcagcgcag cgcccgagcc ctactcgact acggcgtcac cgcggacgcg
     4321 cgcgcgctgc tggcgggaca gcgccacctg ctgacggcgc aggacgagaa cggagacaca
     4381 ccactgcacc tagccatcat ccacgggcag accagtgtca ttgagcagat agtctatgtc
     4441 atccaccacg cccaggacct cggcgttgtc aacctcacca accacctgca ccagacgccc
     4501 ctgcacctgg cggtgatcac ggggcagacg agtgtggtga gctttctgct gcgggtaggt
     4561 gcagacccag ctctgctgga tcggcatgga gactcagcca tgcatctggc gctgcgggca
     4621 ggcgctggtg ctcctgagct gctgcgtgca ctgcttcaga gtggagctcc tgctgtgccc
     4681 cagctgttgc atatgcctga ctttgaggga ctgtatccag tacacctggc ggtccgagcc
     4741 cgaagccctg agtgcctgga tctgctggtg gacagtgggg ctgaagtgga ggccacagag
     4801 cggcaggggg gacgaacagc cttgcatcta gccacagaga tggaggagct ggggttggtc
     4861 acccatctgg tcaccaagct ccgggccaac gtgaacgctc gcacctttgc gggaaacaca
     4921 cccctgcacc tggcagctgg actggggtac ccgaccctca cccgcctcct tctgaaggct
     4981 ggtgctgaca tccatgctga aaacgaggag cccctgtgcc cactgccttc accccctacc
     5041 tctgatagcg actcggactc tgaagggcct gagaaggaca cccgaagcag cttccggggc
     5101 cacacgcctc ttgacctcac ttgcagcacc aaggtgaaga ccttgctgct aaatgctgct
     5161 cagaacacca tggagccacc cctgaccccg cccagcccag cagggccggg actgtcactt
     5221 ggtgatacag ctctgcagaa cctggagcag ctgctagacg ggccagaagc ccagggcagc
     5281 tgggcagagc tggcagagcg tctggggctg cgcagcctgg tagacacgta ccgacagaca
     5341 acctcaccca gtggcagcct cctgcgcagc tacgagctgg ctggcgggga cctggcaggt
     5401 ctactggagg ccctgtctga catgggccta gaggagggag tgaggctgct gaggggtcca
     5461 gaaacccgag acaagctgcc cagcacagag gtgaaggaag acagtgcgta cgggagccag
     5521 tcagtggagc aggaggcaga gaagctgggc ccaccccctg agccaccagg agggctctgc
     5581 cacgggcacc cccagcctca ggtgcactga cctgctgcct gcccccagcc cccttcccgg
     5641 accccctgta cagcgtcccc acctatttca aatcttattt aacaccccac acccacccct
     5701 cagttgggac aaataatcta gaggatccgg ctgtggaatg tgtgtcagtt agggtgtgga
     5761 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5821 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5881 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6061 ttttgcaaaa agcttgggct gcaggtcgag gcggatctga tcaagagaca ggatgagcat
     6121 cgataccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     6181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     6241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     6301 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     6361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     6421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     6481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     6541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     6601 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     6661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     6781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     6841 caagtaagat gcatcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6901 tctggggttc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     6961 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     7021 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
     7081 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     7141 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     7201 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     7261 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     7321 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     7381 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     7441 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     7501 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     7561 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc
     7621 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     7681 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7741 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatctag
     7801 ctaggtagct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     7861 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     7921 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     7981 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     8041 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     8101 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     8161 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     8221 ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat
     8281 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8341 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8401 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8461 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcagcttcag
     8521 aagatggcgg agggcctcca acacagtaat tttcctcccg actcttaaaa tagaaaatgt
     8581 caagtcagtt aagcaggaag tgactaactg accgcagctg gccgtgcgac atcctctttt
     8641 aattagttgc taggcaactg ccctccagag ggcagtgtgg ttttgcaaga ggaagcaaaa
     8701 agcctctcca cccaggccta gaatgtttcc acccaatcat tactatgaca acagctgttt
     8761 tttttagtat taagcagagg ccgggggccc ctggcctccg cttactctgg agaaaaagaa
     8821 gagaggcatt gtagaggctt ccagaggcaa cttgtcaaaa caggactgct tctatttctg
     8881 tcacactgtc tggccctgtc acaaggtcca gcacctccat acccccttta ataagcagtt
     8941 tgggaacggg tgcgggtctt actccgccca tcccgcccct aactccgccc agttccgccc
     9001 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9061 cctctgagct attccagaag tagtgaggag gcttttttgg aggctgcatt aatgaatcgg
     9121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9781 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    10021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    10081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    10141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10381 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
    10441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10681 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    11041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    11101 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    11161 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    11221 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    11281 tgg
//
